中华皮肤科杂志 ›› 2020, Vol. 53 ›› Issue (10): 807-810.doi: 10.35541/cjd.20190983

• 研究报道 • 上一篇    下一篇

外源性胆绿素对中波紫外线诱导的角质形成细胞光损伤的保护作用和机制

张晓卉    林芳    马良娟   

  1. 哈尔滨医科大学附属第二医院皮肤科  150000
    张晓卉现在潍坊市中医院皮肤科，山东  261000

  • 收稿日期:2019-10-14 修回日期:2020-04-05 发布日期:2020-09-30
  • 通讯作者: 马良娟 E-mail:maliangjuan@163.com
  • 基金资助:
    国家自然科学基金（81502706）

Protective effect of exogenous biliverdin on ultraviolet B-induced photodamage to keratinocytes and its mechanisms

Zhang Xiaohui, Lin Fang, Ma Liangjuan   

  1. Department of Dermatology, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150000, China
    Zhang Xiaohui is working on the Department of Dermatology, Weifang Traditional Chinese Hospital, Shandong 261000, China
  • Received:2019-10-14 Revised:2020-04-05 Published:2020-09-30
  • Contact: Ma Liangjuan E-mail:maliangjuan@163.com
  • Supported by:
    National Natural Science Foundation of China （81502706）

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摘要： 【摘要】 目的 探讨外源性胆绿素对中波紫外线（UVB）照射的HaCaT细胞光损伤的保护作用。方法 将HaCaT细胞分为加入0、0.1、1、10 μmol/L胆绿素并照射UVB的UVB组、0.1 μmol/L UVB组、1 μmol/L UVB组、10 μmol/L UVB组及不做处理的对照组。UVB照射剂量为30 mJ/cm2，照射后继续培养24 h，分别检测细胞活性氧（ROS）水平、超氧化物歧化酶（SOD）活力、丙二醛（MDA）含量，ELISA法检测各组细胞的炎症因子白细胞介素6（IL-6）、IL-8水平。多组间均数比较采用单因素方差分析，组间两两比较采用LSD-t检验。结果 UVB组、0.1 μmol/L UVB 组、1 μmol/L UVB组、10 μmol/L UVB组、对照组细胞ROS水平（3 613.33 ± 206.61、2 958.67 ± 193.87、2 678.33 ± 178.24、2 274.67 ± 118.81、1 905.67 ± 250.25）、SOD活力（24.41 ± 1.78、28.96 ± 2.21、29.75 ± 1.75、30.19 ± 2.29、37.52 ± 2.31）、MDA含量（5.61 ± 0.32、5.46 ± 0.55、4.65 ± 0.22、2.55 ± 0.93、1.31 ± 0.05）、IL-6水平、IL-8水平差异均有统计学意义（F值分别为 34.02、57.36、214.09、29.73、11.40，均P < 0.05），UVB组ROS水平、MDA含量及IL-6、IL-8水平均高于另4组（均P < 0.05），SOD活力均低于另4组（均P < 0.05）。结论 外源性胆绿素减轻UVB引起的HaCaT细胞的氧化损伤、减轻炎症反应和抑制脂质过氧化作用，对细胞光损伤有一定的保护作用。

关键词: 紫外线, 角蛋白细胞, 胆绿素, 活性氧, 超氧化物歧化酶, 丙二醛, 白细胞介素6, 白细胞介素8, 炎症因子, 氧化损伤, 脂质过氧化

Abstract: 【Abstract】 Objective To evaluate the protective effect of exogenous biliverdin on ultraviolet B （UVB）-induced photodamage to keratinocytes, and to explore its mechanisms. Methods HaCaT cells were divided into 5 groups: UVB group irradiated with 30 mJ/cm2 UVB alone, 0.1-, 1- and 10-μmol/L UVB groups treated with 0.1, 1, 10 μmol/L biliverdin respectively and irradiated with 30 mJ/cm2 UVB, and control group receiving no treatment. After irradiation, cells in the above groups continued to be cultured for 24 hours. Then, the reactive oxygen species （ROS） level, superoxide dismutase （SOD） activity and malondialdehyde （MDA） content were detected in HaCaT cells, and enzyme-linked immunosorbent assay （ELISA） was performed to detect levels of the inflammatory factors interleukin 6 （IL-6） and IL-8 in HaCaT cells. One-way analysis of variance was used for comparison of means among multiple groups, and least significant difference （LSD）-t test was used for multiple comparisons. Results Significant differences were observed among the UVB group, 0.1-, 1- and 10-μmol/L UVB groups and control group in the ROS levels （3 613.33 ± 206.61, 2 958.67 ± 193.87, 2 678.33 ± 178.24, 2 274.67 ± 118.81, 1 905.67 ± 250.25, respectively, F = 34.02, P < 0.05）, SOD activity （24.41 ± 1.78, 28.96 ± 2.21, 29.75 ± 1.75, 30.19 ± 2.29, 37.52 ± 2.31, respectively, F = 57.36, P < 0.05）, MDA contents （5.61 ± 0.32, 5.46 ± 0.55, 4.65 ± 0.22, 2.55 ± 0.93, 1.31 ± 0.05, respectively, F = 214.09, P < 0.05）, and levels of IL-6 (F = 29.73, P < 0.05) and IL-8 (F = 11.40, P < 0.05). The UVB group showed significantly increased levels of ROS, IL-6 and IL-8, and MDA content compared with the other 4 groups （all P < 0.05）, but significantly decreased SOD activity compared with the other 4 groups （P < 0.05）. Conclusion Exogenous biliverdin has some protective effect on UVB-induced photodamage, likely by reducing oxidative damage to cells, attenuating inflammatory reactions and suppressing lipid peroxidation.

Key words: Ultraviolet rays, Keratinocytes, Biliverdine, Reactive oxygen species, Superoxide dismutase, Malondialdehyde, Interleukin-6, Interleukin-8, Oxidative damage, Inflammatory factors, Lipid peroxidation