中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (7): 426-428.

• 论著 • 上一篇    下一篇

胰岛素样生长因子Ⅱ和Ki-67在先天性皮肤血管瘤及血管畸形中的表达

李延, 许成蓉, 周礼义   

  1. 华中科技大学同济医学院附属同济医院皮肤科 武汉 430030
  • 收稿日期:2004-07-17 发布日期:2005-07-15

Expression of Insulin-like Growth Factor 2 and Ki-67 in Hemangioma and Vascular Malformation

LI Yan, XV Cheng-rong, ZHOU Li-yi   

  1. Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
  • Received:2004-07-17 Published:2005-07-15

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摘要： 目的 探讨胰岛素样生长因子Ⅱ(IGF-Ⅱ)在血管瘤增殖与退化中的作用。方法 应用免疫组化方法检测52例血管瘤、27例血管畸形和5例正常皮肤标本中IGF-Ⅱ及细胞增殖核抗原Ki-67的表达情况。同时应用RT-PCR法检测9例血管瘤、5例血管畸形和5例正常皮肤标本中IGF-ⅡmRNA的表达情况。结果 免疫组化结果显示血管瘤组的IGF-Ⅱ和Ki-67表达均高于血管畸形组及正常皮肤组(P<0.01)。血管瘤组中增生期亚组两标记指数均高于退化期亚组(P<0.01)。血管瘤组中IGF-Ⅱ与Ki-67标记指数的相关系数为0.84,P<0.01。RT-PCR法检测到增殖期血管瘤中有明显IGF-ⅡmRNA的表达,而消退期血管瘤、血管畸形和正常皮肤中未见明显IGF-ⅡtuRNA的表达。结论 IGF-Ⅱ与血管瘤内皮细胞的增殖相关。

关键词: 血管瘤, 血管发育不良, 胰岛素样生长因子Ⅱ, Ki-67抗原

Abstract: Objective To study the role of insulin-like growth factor 2 (IGF-Ⅱ) in the proliferation and degeneration of hemangiomas.Methods The expression of IGF-Ⅱ and Ki-67 was detected with SP im-munohistochemical method in 52 specimens of hemangiomas,27 of vascular malformation and 5 of normal skin.The expression of IGF-Ⅱ mRNA was examined by reverse transcription polymerase chain reaction (RT-PCR) in specimens from 9 cases of hemangiomas and 5 of vascular malformation.Results The labelling indexes (LI) of IGF-Ⅱ and Ki-67 and the expression of IGF-Ⅱ mRNA were significantly higher in heman gioma than those in vascular malformation and normal skin (P<0.01).LI were also higher in hemangioma at proliferative stage than those at degenerative stage (P<0.01).There was significantly positive correlation between LI of IGF-Ⅱ and Ki-67 in hemangioma (r=0.84,P<0.01).The expression of IGF-Ⅱ mRNA was significantly increased in hemangioma at proliferative stage,and not obvious in hemangioma at degenerative stage,vascular malformation and normal skin.Conclusion IGF-Ⅱ is related to the proliferation of vascular endothelial cells in hemangioma.

Key words: Hemagioma, Angiodysplasia, Insulin-like growth factor Ⅱ, Ki-67 antigen