中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (3): 163-164.

• 论著 • 上一篇    下一篇

荨麻疹患者白介素13的检测

施若非, 李卫平, 潘萌, 李霞, 郑捷   

  1. 上海第二医科大学附属瑞金医院皮肤科 200025
  • 收稿日期:2004-01-05 出版日期:2005-03-15 发布日期:2005-03-15

Determination of IL-13 in Urticaria Patients

SHI Ruo-fei, LI Wei-ping, PAN Meng, LI Xia, ZHENG Jie   

  1. Department of Dermatology and Rheumatology, Rui Jin Hospital of Shanghai Second Medical University, Shanghai 200025, China
  • Received:2004-01-05 Online:2005-03-15 Published:2005-03-15

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摘要： 目的 研究白介素(IL)-13在急、慢性荨麻疹患者中的作用。方法 对22例急性荨麻疹患者和20例慢性荨麻疹患者,采用流式细胞仪检测淋巴细胞内IL-13、IL-4、干扰素酌(IFN-酌);用ELISA法测定血清IL-13和总IgE,并与正常人对照组进行比较。结果 经流式细胞仪检查发现,急性荨麻疹患者淋巴细胞IL-13的表达显著升高,与正常人对照组差异有统计学意义;而慢性荨麻疹患者的IL-13表达略有升高,与正常人对照组差异无统计学意义;IL-4的表达在两组患者中差异均无统计学意义。ELISA检测血清IL-13的结果与流式细胞仪检测结果相符;急慢性荨麻疹患者IL-4的表达与正常人对照组差异无统计学意义;IFN-酌的表达在荨麻疹患者中无明显改变。IgE的表达在急、慢性患者中均显著升高。但IL-13的表达和IgE抗体的量无相关性。结论 IL-13在荨麻疹的发病中发挥了重要作用,尤其是急性荨麻疹。

关键词: 荨麻疹, 白细胞介素13, 白细胞介素4

Abstract: Objective To investigate the role of IL-13 in the patients with acute and chronic urticaria.Methods In 22 patients with acute urticaria,20 patients with chronic urticaria and 19 normal controls,the levels of IL-13,IL-4 and IFN-γ of peripheral T lymphocytes were measured by flow cytometry.The serum concentrations of IL-13 and total IgE were tested by ELISA.Results The Results of flow cytometry showed that the level of IL-13 of the patients with acute urticaria was significantly higher than that of the normal controls (P<0.05).Although the expression level of IL-13 also increased in the patients with chronic urticaria,there was no significant difference compared with that of the normal controls.There was no significant difference in the expiession level of IL-4 between the acute and chronic urticaria patients.The IL-13 Results measured by ELISA were consistent with those by flow cytometry.There were no changes in the expression of IFN-γ in these patients.The total serum IgE level of patients with acute and chronic urticaria was significantly higher than that of normal controls (P<0.01).Conclusion IL-13 may play an important role in the pathogenesis of urticaria,especially in acute urticaria.

Key words: Urticaria, Interleukin-13, Interleukin-4