中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (3): 160-162.

• 论著 • 上一篇    下一篇

白念珠菌ERG11基因启动子部分序列分析

乔建军, 张宏   

  1. 暨南大学附属第一医院皮肤科 广州 510632
  • 收稿日期:2003-12-03 出版日期:2005-03-15 发布日期:2005-03-15
  • 基金资助:
    国家自然科学基金资助项目(30070690)

Analysis on Partial Sequence of ERG11 Promoter Region of Candida albicans

QIAO Jian-jun, ZHANG Hong   

  1. Department of Dermatology, First Affiliated Hospital of Jinan University, Guangzhou 510632, China
  • Received:2003-12-03 Online:2005-03-15 Published:2005-03-15

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摘要： 目的 探讨白念珠菌菌丝相与酵母相ERG11基因启动子部分(-440～-1)碱基序列的差异以及ERG11基因启动子突变与白念珠菌对氟康唑敏感性的关系。方法 分别提取从同一亲本来源、对氟康唑敏感性不同的白念珠菌菌丝相与酵母相基因组DNA,根据ERG11基因启动子序列及其编码序列设计一对引物,对ERG11基因上游启动子部分碱基序列(-503～53)进行PCR扩增,经PCR产物直接测序比较白念珠菌菌丝相与酵母相ERG11基因启动子序列(-440～-1)的差异以及对氟康唑敏感性不同的白念珠菌ERG11基因启动子序列的差异。结果 白念珠菌菌丝相与酵母相ERG11启动子序列未发现差异。氟康唑敏感株白念珠菌ERG11基因启动子的-365,-353,-328,-310,-308,-299,-295,-293,-292,290,-289位点为杂合状态,且在-284位点有一等位基因发生单碱基缺失(-284delT);而在白念珠菌氟康唑剂量依赖性敏感和耐药株上述杂合现象消失。结论 白念珠菌菌丝相与酵母相ERG11基因启动子-440～-1区碱基序列无差异;ERG11基因启动子突变可能与白念珠菌对氟康唑耐药有关。

关键词: 菌丝, 酵母相, 念珠菌,白色, 启动子区(遗传学), DNA突变分析, 氟康唑

Abstract: Objectives To compare the difference of ERG11 partial promoter sequence(-440～-1)between yeast-form and hypha-form of Candida albicans,and the relation between the mutation of ERG11 promoter and the susceptibility of C.albicans to fluconazole.Methods Yeast-form and hypha-form of C.albicans genome DNA were extracted from 4 substrains C.albicans,which were isolated from a single HIV-sero-positive patient.Partial ERG11 promoters of yeast-form and hypha-form of these 4 substrains were amplified by polymerase chain reaction (PCR) and the PCR products were sequenced.Results There was no sequence difference between the yeast-form and the hypha-form of C.albicans in this region.Heterogeneities at position -365,-353,-328,-310,-308,-299,-295,-293,-292,290,-289 and a single base deletion (-284 delT) were found on one allele of ERG11 promoter region of fluconazole-susceptible substrains.No heterogeneities were observed in dose-dependent fluconazole-susceptible and fluconazole-resistant substrains.Conclusions There is no difference of ERG11 partial promoter sequence between the yeast-form and the hypha-form C.albicans.The mutation of ERG11 promoter may be associated with the resistance of C.albicans to fluconazole.

Key words: Yeast-form, Hyphae, Candida albicans, Promoter regions(Genetics), DNA mutational analysis, Fluconazole