中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (3): 165-167.

• 论著 • 上一篇    下一篇

D-半乳糖诱导大鼠皮肤衰老的形态学与生化指标观察

石丽君1, 吴铁2, 吴志华2   

  1. 1. 广东医学院药理教研室 广东湛江 524023;
    2. 广东医学院附属医院皮肤科 广东湛江 524023
  • 收稿日期:2004-01-15 出版日期:2005-03-15 发布日期:2005-03-15

Morphology and Biochemical Parameters in Rats with D-galactose-induced Skin Ageing

SHI Li-jun1, WU Tie2, WU Zhi-hua2   

  1. Department of Pharmacology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China
  • Received:2004-01-15 Online:2005-03-15 Published:2005-03-15

PDF

160

赞

摘要： 目的 观察D-半乳糖诱导皮肤衰老大鼠的皮肤形态学改变及与衰老相关的生化指标变化。方法 26只SD雌雄大鼠随机分为2组:D-半乳糖组每天给予D-半乳糖皮下注射120mg·kg-1·d-1,对照组皮下注射等量生理盐水。喂养100d后处死大鼠,观察血液和皮肤中与衰老相关的生化指标,并行皮肤组织病理学检查,用计算机图像分析系统定量分析表皮厚度、弹力纤维的面积。结果 D-半乳糖组大鼠表皮变薄,胶原纤维及弹力纤维减少,排列疏松,呈现与人类皮肤衰老相似的表现。D-半乳糖组大鼠皮肤中羟脯氨酸含量降低,丙二醛含量增加,全血谷胱甘肽过氧化物酶和过氧化氢酶活性降低,红细胞中超氧化物歧化酶活性降低。结论 D-半乳糖120mg·kg-·1d-1皮下注射100d可导致大鼠皮肤衰老样改变。

关键词: 皮肤衰老, 半乳糖

Abstract: Objective To observe the changes of morphology and biochemical parameters in rats with D-galactose-induced skin ageing.Methods Twenty-six female and male rats were randomly divided into 2 groups.D-galactose group rats were injected subcutaneously with 120 mg·kg-1·d-1 of D-galactose everyday,whereas the control group with normal saline.The rats were sacrificed on days 100.The ageing-related biochemical parameters were detected in the blood and skin,and the cutaneous histopathology was observed.Epidermal thickness and area of elastic fibers were determined quantitatively with imaging analysis system.Results Epidermal thickness and content of elastic fibers were significantly lower in D-galactose group than those in the control group.The loose arrangement of collagen and elastic fibers was evident in D-galactose group.These features of the rats in D-galactose group were similar to those in the ageing skin of human beings.Compared with the control group,the levels of hydroxyproline decreased and malondialdehyde (MDA) increased in the skin of the D-galactose group.The activity of glutathione peroxidase (GSH-PX) and catalase (CAT) in the whole blood and that of superoxide dismutase (SOD) in erythrocytes were remarkably reduced in the D-galactose group as compared to the control group.Conclusions The rat models with skin ageing are established by subcutaneous injection of 120 mg·kg-1·d-1 of D-galactose once daily for 100 days.The changes of morphologic and biochemical parameters in these models are analogous to those in human skin ageing.

Key words: Skin aging, Galactose