中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (4): 215-217.

• 论著 • 上一篇    下一篇

银屑病患者皮损表皮细胞促凋亡分子PDCD5表达的研究

何焱玲1, 齐焕英1, 闻卫兢1, 王卉2, 朱铁君1   

  1. 1. 北京大学人民医院皮肤科;
    2. 北京大学人民医院血液病研究所
  • 收稿日期:2003-04-16 出版日期:2004-04-15 发布日期:2004-04-15
  • 基金资助:
    国家985项目基金北京大学人类疾病基因中心资助课题(2002-T2)

Study on the Expression of Apoptosis-Related Molecule PDCD5 in the Skin Lesions of Psoriasis

HE Yan-ling1, QI Huan-ying1, WEN Wei-jing1, WANG Hui2, ZHU Tie-jun1   

  1. Department of Dermatology, Peking University People Hospital, Beijing 100044, China
  • Received:2003-04-16 Online:2004-04-15 Published:2004-04-15

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摘要： 目的 探讨银屑病患者表皮细胞过度增殖与细胞凋亡分化调节异常的关系.方法 寻常性银屑病患者30例,正常人对照28例.采用直接免疫荧光观察促凋亡分子PDCD5的表达状态,流式细胞仪和计算机CELL Quest软件定量分析表皮单细胞表达PDCD5的平均荧光强度和阳性率,逆转录聚合酶链反应方法检测表皮细胞表达PDCD5 mRNA的变化.结果 银屑病患者皮损增生的表皮组织细胞内PDCD5蛋白的表达明显降低,表皮单细胞表达PDCD5促凋亡分子的平均荧光强度和阳性率较正常人显著降低(P<0.01),皮损组织细胞表达PDCD5 mRNA显著低于正常人.结论 银屑病患者局部皮损表皮细胞的过度增殖与促凋亡分子PDCD5表达的下调有关,与表皮细胞的凋亡及终末分化过程的调节异常密切相关.

关键词: 银屑病, 角蛋白细胞, 细胞凋亡

Abstract: Objective To study the relationship between apoptosis regulation and epidermal proliferation in psoriasis. Methods The expression of apoptosis molecule PDCD5 (programmed cell death 5) on psoriatic lesions was observed by direct immunofluorescence. The positive rates and the average of fluorescence intensity of PDCD5 were analyzed quantitatively with FACS and computer CELL Quest software. The expression of PDCD5 mRNA in psoriatic lesions was detected by RT-PCR. Results The expression of PDCD5 protein was obviously lower in psoriatic epidermal cells than that of the normal skin. The positive rate and the average fluorescence intensity of PDCD5 in psoriatic epidermal cells were notably lower than those of the normal skin (P<0.01). The expression of PDCD5 mRNA in psoriatic lesions was lower than that of the normal skin. Conclusions The epidermal over-proliferation of psoriatic lesions is correlated with the down-regulation of apoptosis molecule PDCD5 expression, which indicates an abnormal regulation of epidermal cell differentiation.

Key words: Psoriasis, Keratinocytes, Apoptosis