中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (2): 98-100.

• 论著 • 上一篇    下一篇

金黄色葡萄球菌性超抗原诱发银屑病发病机理探讨

张峻岭1, 张理涛1, 陈学荣2, 殷金珠3   

  1. 1. 天津市长征医院皮肤科 300021;
    2. 北京大学第三医院皮肤科;
    3. 北京大学免疫室
  • 收稿日期:2000-04-06 出版日期:2001-04-15 发布日期:2001-04-15

Study on the Pathogenesis of Psoriasis Induced by Staphylococcal Superantigen

ZHANG Junling1, ZHANG Litao1, CHEN Xuerong2   

  1. Department of Dermatology, Tianji n Changzheng Hospital, Tianjin 300021, China
  • Received:2000-04-06 Online:2001-04-15 Published:2001-04-15

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摘要： 目的 探讨金黄色葡萄球菌性超抗原诱发银屑病的机制。方法 3H-TdR掺入法测定细胞增殖反应,亚二倍体细胞含量测定,片段化DNA分析,膜联蛋白V(Annexin V)法测定细胞凋亡,流式细胞仪检测细胞表面抗原表达。结果 金黄色葡萄球菌肠毒素B活化的淋巴细胞培养上清液作用于角质形成细胞48h,可促进角质形成细胞增殖,诱导角质形成细胞表达HLA-DR和Fas抗原;再次加入上清液继续作用48h,则诱导角质形成细胞凋亡(P<0.01)。结论 金黄色葡萄球菌肠毒素B作为超抗原活化T细胞,使之释放细胞因子,后者使角质形成细胞首先活化增殖,继而凋亡。

关键词: 银屑病, 葡萄球菌, 金黄色, 超抗原, 角蛋白细胞, 凋亡

Abstract: Objective To investigate the pathogenesis of psoriasis induced by staphylococcal superantigen. Methods The proliferation responses of keratinocyte s were measured by 3H-TdR incorporation assay. Quantification of hypodiploid c ells, analysis of DNA fragmentation, and annexin V method were employed to detec t cell apoptosis. The expression of surface antigens on keratinocytes was determ ined by flow cytometric assay. Results The supernatants of peripheral blood lymp hocytes stimulated by staphylococcal enterotoxins B(SEB) could promote the proli feration of keratinocytes and induce the expression of HLA-DR and Fas antigens on keratinocytes after the first 48 h of treatment. Apoptosis of keratinocytes was induced after addition of the same supernatants(P<0.01) for another 48 h. Conclusions Cytokines released by T lymphocytes activated by SEB, a staphyloco ccal superantigen, may firstly stimulate the proliferation, and then induce apop tosis of kera tinocytes.

Key words: Psoriasis, Superantigens, Keratinocytes, Apoptosis, Staphylococcus,aureus