中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (4): 240-242.

• 论著 • 上一篇    下一篇

c-myc、p53和bcl-2与银屑病角质形成细胞凋亡异常关系的研究

倪晓, 孙建方, 曾学思, 桑红桂, 李阿梅, 叶干运   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:1999-09-08 出版日期:2000-08-15 发布日期:2000-08-15
  • 基金资助:
    卫生部基金资助课题

Study on the Relationship Between c-myc, p53 and bcl-2 and Abnormal Apoptosis of Keratinocytes in Psoriatic Lesions

NI Xiao, SUN Jianfang, ZENG Xuesi   

  1. Department of Pathology, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042
  • Received:1999-09-08 Online:2000-08-15 Published:2000-08-15

摘要: 目的 探讨原癌基因c-myc、抑癌基因p53和凋亡保护基因bcl-2在银屑病角质形成细胞凋亡异常中的作用。方法 采用免疫组化和非同位素核酸原位杂交技术从蛋白和mRNA二个水平检测了36份寻常型银屑病皮损中c-myc、p53和bcl-2的表达状况。结果 银屑病表皮中的c-myc、p53和增殖细胞核抗原(PCNA)阳性细胞较正常皮肤明显增加,而bcl-2阳性细胞在表皮基底层中明显减少。在PCNA阳性细胞>30的银屑病皮损中,有更多的c-myc阳性细胞(P<0.01)、更多的p53阳性细胞(P<0.01),bcl-2的表达明显下降(P<0.02)及更多的凋亡诱导(P<0.01)。结论 银屑病时c-myc、p53的表达上调和bcl-2的表达下调可能共同激活了角质形成细胞的凋亡通路,银屑病时角质形成细胞的凋亡增加与异常活化和增殖密切相关。

关键词: 基因,c-myc, 基因,p53, 基因,bcl-2, 银屑病, 脱噬作用

Abstract: Objective To investigate the effects of proto-oncogene c-myc, tumor suppressor gene p53 and apoptosis protection gene bcl-2 on abnormal apoptosis of keratinocytes in psoriatic lesions. Methods Expression of c-myc, p53, bcl-2 was analysed by both immunohistochemical methods and nucleic acid in situ hybridization in a total of thirty six lesional skin samples from patients with psoriasis vulgaris. Results c-myc, p53 and PCNA-positive keratinocytes increased significantly in psoriatic lesions in comparison with the controls,especially in the lower and basal layers of epidermis. bcl-2-positive cells decreased in basal layers of psoriatic skin. In lesions with more than 30 PCNA-positive cells, there were a significant increase of c-myc protein (P<0.01) and p53 protein (P<0.01), a significant decline of bcl-2 protein (P<0.02), and a significantly high rate of apoptosis (P<0.01). Conclusions The increased expression of c-myc, p53 and the decreased expression of bcl-2 may play a role in coordinated activation of apoptotic pathways in psoriatic skin. The abnormal apoptosis of keratinocytes is likely to relate to hyperplasia of keratinocytes in the psoriatic lesions.

Key words: Genes,c-myc, Genes,p53, Genes,bcl-2, Psoriasis, Apoptosis