中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (2): 99-100.

• 论著 • 上一篇    下一篇

β溶血型链球菌诱发点滴型银屑病发病机制的研究

张理涛1, 陈学荣1, 殷金珠2   

  1. 1. 北京医科大学第三医院皮肤科 100083;
    2. 北京医科大学临床免疫室
  • 收稿日期:1997-12-01 修回日期:1998-08-07 出版日期:1999-04-15 发布日期:1999-04-15

Study on Pathogenesis of Guttate Type Psoriasis Induced by Group A β-haemolytic Streptococcal Infection

ZHANG Litao1, CHEN Xuerong1, YIN Jinzhu2   

  1. Department of Dermatology, The Third School of Clinical Medicine, Beijing Medical University, Beijing 100083
  • Received:1997-12-01 Revised:1998-08-07 Online:1999-04-15 Published:1999-04-15

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摘要： 目的 探讨β溶血型链球菌所诱发的急性点滴型银屑病的发病机制.方法 应用MTT方法观察急性点滴型银屑病患者外周血淋巴细胞(PBMC)对链球菌抗原的反应,用3H-TdR掺入法和免疫组织化学方法检测链球菌抗原刺激的PBMC培养上清液对人类角质形成细胞株COLO16DNA合成以及HLA-DR、ICAM-1分子表达的影响.结果 急性点滴型银屑病患者PBMC对链球菌抗原的反应较对照组更为明显(P<0.01),其24h链球菌抗原刺激的PBMC培养上清液对角质形成细胞的促增殖作用较对照组显着增强(P<0.01),并能诱导角质形成细胞表达HLA-DR和ICAM-1.结论具有银屑病素质的个体体内可能存在链球菌抗原特异性的T淋巴细胞,受链球菌抗原或超抗原作用后增殖,产生大量细胞因子,导致角质形成细胞过度增生和HLA-DR、ICAM-1异常表达.

关键词: 银屑病, 链球菌属

Abstract: Objective To investigate the pathogenesis of acuteguttate type psoriasis(AGP) in duced by streptococcal infection. Methods The proliferative responses of peripheral blood mononuclear cells (PBMCs) to streptococcal antigens were detected by using MTT methods.Effects of supernatants of PBMCs cultured with streptococcal antigens on DNA synthesis of keratinocytes were measured by 3H-TdR incorporation assay,and immunohistochemical staining was used to examine HLA-DR and intercellular adhesion molecule 1(ICAM 1) expression on keratinocytes. Results The proliferative responses of PBMCs to streptococcal antigens were greatly enhanced in patients with AGP than those in controls (P<0.01).The supernatants of PBMCs in patients with AGP stimulated by streptococcalantigens could significantly increase 3H-TdR incorporation with keratinocytes(P<0.01).And these supernatants from patients with AGP could also induce the expression of HLA-DR and ICAM-1 in human keratinocytes. Conclusion There might be streptococcal antigen-specific T lymphocytes in AGP patients.

Key words: Psoriasis, Streptococcus