中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (2): 82-84.

• 论著 • 上一篇    下一篇

银屑病皮损区IL-8、HLA-DR、PLA2、ANAE、EGFR mRNA、c-fos mRNA的定位研究

李红文1, 吴景兰2, 丁一2, 王一菱2, 时建业3, 王诗淇1, 付世珍1   

  1. 1. 河南医科大学第一附属医院皮肤科 郑州 450052;
    2. 河南医科大学细胞分子生物学中心;
    3. 河南禹州禹灵集团
  • 收稿日期:1998-05-26 修回日期:1998-11-13 出版日期:1999-04-15 发布日期:1999-04-15
  • 基金资助:
    河南省卫生厅资助

Study on Localization of IL-8、HLA-DR、PLA2、ANAE、EGFR mRNA and c-fos mRNA in Psoriatic Lesions

LI Hongwen1, WU Jinglan2, DING Yi2   

  1. Department of Dermatology, First Affiliated Hospital, Henan Medical University, Zhengzhou 450052
  • Received:1998-05-26 Revised:1998-11-13 Online:1999-04-15 Published:1999-04-15

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摘要： 目的 探讨银屑病患者白介素8(IL-8)、人类白细胞抗原DR(HLA-DR)、磷脂酶A2(PLA2)、酸性α-乙酸萘酯酶(ANAE)、表皮生长因子受体基因(EGFRmRNA)、c-fos基因(c-fosmRNA)在皮损的表达定位.方法 对20例银屑病患者皮损和10例正常人的皮肤标本进行了免疫组化、组化和原位杂交技术检测.结果 IL8免疫反应主要定位于表皮的基层、棘细胞层和颗粒层、血管内皮细胞及间质细胞;人类白细胞抗原DR免疫反应及ANAE分型染色主要定位于浸润细胞[包括单核巨噬细胞及T辅助淋巴细胞(TH细胞)]和血管内皮细胞;PLA2活性主要定位于表皮细胞的胞膜及角层和间质细胞;以上观察指标在皮损区反应均较强或反应细胞较多;c-fosmRNA的杂交信号皮损区弱于对照区;EGFRmRNA在皮损区杂交信号虽较强,但与对照组相比差异不显着.结论 IL-8及其介导的免疫炎症反应在银屑病发病中起着十分重要的作用,并使PLA2活性增强,淋巴细胞激活.c-fos原癌基因在增殖的角质形成细胞中呈弱表达,提示其终末分化较差.而EGFR mRNA的表达强度则与银屑病无显着相关关系.而IL-8与HLADR和TH细胞之间呈明显正相关(P<0.01).

关键词: 银屑病, 白细胞介素-8, HLA-DR, 磷脂酶A类, 基因,fos, 基因,ERBB-1

Abstract: Objective To study the localization of interleukin-8 (IL-8), HLA-DR, phospholipase A2 (PLA2), acid α naphthyl acetate esterase (ANAE), epidermal growth factor receptor mRNA (EGFR mRNA), c fos mRNA in psoriatic skin. Methods Skin lesions of 20 psoriatic cases who received no treatment in previous three months and skin samples of 10 controls were detected by histochemistry, immunohistochemistry and in situ hybridization technique. Results IL-8 was mainly localized at the basal cell layer, spinous cell layer and granular cell layer of the epidermis, the endothelium of the blood vessels and interstitial cells; HLA-DR and ANAE were mainly localized at the infiltrating immune cells in dermis (including TH cells and mononuclear phagocytes) and the endothelial cells of the blood vessels; PLA2 reaction was mainly seen at the cytomembrane of keratinocytes in deeper epidermal layers, cornified layer and dermal interstitial cells. All above mentioned positive reactions were more intense, or there were more positive cells in psoriatic lesions than those in controls. c-fos mRNA signal were weaker in lesions than that in controls. EGFR mRNA signal was more intense in lesions, but there was no significant difference between those in the lesions and in controls. Conclusion The results suggest that IL-8 and its mediated inflammatory reactions might play an important role in the development of psoriasis, and it also enhances PLA2 activity and activates lymphocytes. c fos protooncogene is weakly expressed in proliferative keratinocytes. No distinctive correlation between EGFR mRNA and the development of psoriasis is found.

Key words: Psoriasis, Interleukin-8, HLA-DR, Phospholipases A, Genes,fos, Genes,erbB-1