中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (2): 72-74.

• 论著 • 上一篇    下一篇

银屑病患者皮损中端粒酶活性的测定

程浩, 茅晓红, 张行, 蔡心涵, 孙国均, 郑树   

  1. 1. 浙江医科大学附属第二医院皮肤科, 杭州 310009;
    2. 浙江医科大学肿瘤研究所, 杭州 310009
  • 收稿日期:1997-08-19 修回日期:1997-11-03 出版日期:1998-04-15 发布日期:1998-04-15

Detection of Telomerase Activity in the Lesion of Psoriasis

Cheng Hao, Mao Xiaohong, Zhang Xing   

  1. Department of Dermatology, Second Affliated Hospital and Cancer Research Institute, Zhejiang Medical University, Hangzhou 310009
  • Received:1997-08-19 Revised:1997-11-03 Online:1998-04-15 Published:1998-04-15

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摘要： 目的 探讨银屑病的端粒酶活性表达情况。方法 采用端粒重复序列扩增文件——酶标法(TRAP-ELISA)测定银屑病皮损中端粒酶活性。结果 银屑病皮损中有一定水平的端粒酶活性,但其水平显著低于肿瘤组织和细胞株。结论 端粒酶不仅可在恶性肿瘤表达,也可在一些非恶性皮肤病组织表达;并且认为银屑病皮损中端粒酶活性的增加可能与银屑病发病机理尤其是表皮细胞的持续过度分化有某种关联。

关键词: 银屑病, DNA核苷酸外转移酶

Abstract: Objective To investigate telomerase activity in patients with psoriasis. Methods The telomerase activity in the skin lesions of patients was detected by TRAP (Telomeric repeat amplification protocol)——ELISA which is based upon PCR amplification of the initial telomerase product and detected by ELISA. Results Telomerase activity was detected in the lesions of psoriatic patients, but the level of it was lower in comparison with that in tissues of malignant tumor and K562 cell line. Conclusion These findings support that the increase of telomerase activity may be linked to the pathogenesis of psoriasis and that the telomerase activity can be detected not only in malignant tumors but also in nonmalignant skin diseases.

Key words: Psoriasis, DNA nucleotidylexotransferase