间充质干细胞三维培养技术及其在皮肤病治疗中的应用进展

doi:10.35541/cjd.20230083

中华皮肤科杂志 ›› 2023, e20230083.doi: 10.35541/cjd.20230083

间充质干细胞三维培养技术及其在皮肤病治疗中的应用进展

王琪坚哲

空军军医大学西京皮肤医院，西安 710032

收稿日期:2023-02-17 修回日期:2023-04-06 发布日期:2023-07-21
通讯作者: 坚哲 E-mail:xjzhejian@fmmu.edu.cn
基金资助:
国家自然科学基金（No.82173414）；陕西省自然科学基础研究计划重点项目（S2023-JC-ZD-0226）；空军军医大学凌云工程翱翔计划（axjhjz-102）

Three-dimensional culture techniques of mesenchymal stem cells and their application in the treatment of skin diseases

Wang Qi, Jian Zhe

Received:2023-02-17 Revised:2023-04-06 Published:2023-07-21
Contact: Jian Zhe E-mail:xjzhejian@fmmu.edu.cn
Supported by:
National Natural Science Foundation of China （82173414）; Key Projects of Shaanxi Natural Science Basic Research Program （S2023-JC-ZD-0226）; Flying Plan of Lingyun Project of Air Force Military Medical University （axjhjz-102）

摘要/Abstract

摘要： 【摘要】间充质干细胞（MSC）具有再生修复和免疫调节功能。三维培养MSC提供了更符合人体的细胞生存环境，有效促进细胞增殖、分化和旁分泌能力，提高了细胞的各种生物学特性。本文综述MSC相关的三维培养技术及其在皮肤病中的应用进展，为MSC三维培养方法的选择及其在皮肤疾病中的临床应用提供借鉴。

关键词: 间质干细胞, 细胞培养技术, 三维培养, 皮肤损伤与修复, 光老化, 自身免疫疾病, 红斑狼疮, 系统性

Abstract: 【Abstract】 Mesenchymal stem cells （MSCs） have regenerative repair and immunoregulatory capabilities. Three-dimensional culture techniques provide a more human-friendly environment for cell living, can effectively promote cell proliferation, differentiation and paracrine capabilities, and improve various biological characteristics of MSCs. This review summarizes three-dimensional culture techniques of MSCs and their application in skin diseases, and provides a reference for the selection of three-dimensional culture methods for MSCs and their clinical application in skin diseases.

Key words: Mesenchymal stem cells, Cell culture techniques, Three-dimensional culture, Skin damage and repair, Photoaging, Autoimmune diseases, Lupus erythematosus, systemic

王琪坚哲. 间充质干细胞三维培养技术及其在皮肤病治疗中的应用进展[J]. 中华皮肤科杂志, 2023,e20230083. doi:10.35541/cjd.20230083

Wang Qi, Jian Zhe. Three-dimensional culture techniques of mesenchymal stem cells and their application in the treatment of skin diseases[J]. Chinese Journal of Dermatology,2023,e20230083. doi:10.35541/cjd.20230083

参考文献 44

［1］	Dörnen J, Dittmar T. The role of MSCs and cell fusion in tissue regeneration［J］. Int J Mol Sci, 2021,22（20）:10980. doi: 10. 3390/ijms222010980.
［2］	Shen Z, Huang W, Liu J, et al. Effects of mesenchymal stem cell⁃derived exosomes on autoimmune diseases［J］. Front Immunol, 2021,12:749192. doi: 10.3389/fimmu.2021.749192.
［3］	Lee BC, Kang I, Yu KR. Therapeutic features and updated clinical trials of mesenchymal stem cell （MSC）⁃derived exosomes［J］. J Clin Med, 2021,10（4）doi: 10.3390/jcm10040711.
［4］	Huang X, Huang Z, Gao W, et al. Current advances in 3D dynamic cell culture systems［J］. Gels, 2022,8（12）:829. doi: 10.3390/gels8120829.
［5］	Farhat J, Pandey I, AlWahsh M. Transcending toward advanced 3D⁃cell culture modalities: a review about an emerging paradigm in translational oncology［J］. Cells, 2021,10（7）:1657. doi: 10. 3390/cells10071657.
［6］	Jauković A, Abadjieva D, Trivanović D, et al. Specificity of 3D MSC spheroids microenvironment: impact on msc behavior and properties［J］. Stem Cell Rev Rep, 2020,16（5）:853⁃875. doi: 10.1007/s12015⁃020⁃10006⁃9.
［7］	Bagheri⁃Hosseinabadi Z, Seyedi F, Mollaei HR, et al. Combi⁃nation of 5⁃azaytidine and hanging drop culture convert fat cell into cardiac cell［J］. Biotechnol Appl Biochem, 2021,68（1）:92⁃101. doi: 10.1002/bab.1897.
［8］	Velasco V, Shariati SA, Esfandyarpour R. Microtechnology⁃based methods for organoid models［J］. Microsyst Nanoeng, 2020,6:76. doi: 10.1038/s41378⁃020⁃00185⁃3.
［9］	Mehta G, Hsiao AY, Ingram M, et al. Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy［J］. J Control Release, 2012,164（2）:192⁃204. doi: 10.1016/j.jconrel.2012.04.045.
［10］	Gionet⁃Gonzales MA, Leach JK. Engineering principles for guiding spheroid function in the regeneration of bone, cartilage, and skin［J］. Biomed Mater, 2018,13（3）:034109. doi: 10.1088/1748⁃605X/aab0b3.
［11］	Costa EC, de Melo⁃Diogo D, Moreira AF, et al. Spheroids formation on non⁃adhesive surfaces by liquid overlay technique: considerations and practical approaches［J］. Biotechnol J, 2018,13（1）:1700417. doi: 10.1002/biot.201700417.
［12］	Pettinato G, Wen X, Zhang N. Formation of well⁃defined embryoid bodies from dissociated human induced pluripotent stem cells using microfabricated cell⁃repellent microwell arrays［J］. Sci Rep, 2014,4:7402. doi: 10.1038/srep07402.
［13］	Frantz C, Stewart KM, Weaver VM. The extracellular matrix at a glance［J］. J Cell Sci, 2010,123（Pt 24）:4195⁃4200. doi: 10.1242/jcs.023820.
［14］	Xing H, Lee H, Luo L, et al. Extracellular matrix⁃derived biomaterials in engineering cell function［J］. Biotechnol Adv, 2020,42:107421. doi: 10.1016/j.biotechadv.2019.107421.
［15］	Habanjar O, Diab⁃Assaf M, Caldefie⁃Chezet F, et al. 3D cell culture systems: tumor application, advantages, and disadvan⁃tages［J］. Int J Mol Sci, 2021,22（22）:12200. doi: 10.3390/ijms222212200.
［16］	Shen H, Cai S, Wu C, et al. Recent advances in three⁃dimensional multicellular spheroid culture and future development［J］. Micromachines （Basel）, 2021,12（1）:96. doi: 10.3390/mi12010096.
［17］	Caliari SR, Burdick JA. A practical guide to hydrogels for cell culture［J］. Nat Methods, 2016,13（5）:405⁃414. doi: 10.1038/nmeth.3839.
［18］	Orive G, Santos E, Pedraz JL, et al. Application of cell encapsulation for controlled delivery of biological therapeutics［J］. Adv Drug Deliv Rev, 2014,67⁃68:3⁃14. doi: 10.1016/j.addr.2013.07.009.
［19］	Hu X, Xia Z, Cai K. Recent advances in 3D hydrogel culture systems for mesenchymal stem cell⁃based therapy and cell behavior regulation［J］. J Mater Chem B, 2022,10（10）:1486⁃1507. doi: 10.1039/d1tb02537f.
［20］	Milovac D, Gallego Ferrer G, Ivankovic M, et al. PCL⁃coated hydroxyapatite scaffold derived from cuttlefish bone: morphology, mechanical properties and bioactivity［J］. Mater Sci Eng C Mater Biol Appl, 2014,34:437⁃445. doi: 10.1016/j.msec.2013.09.036.
［21］	Li P, Fu L, Liao Z, et al. Chitosan hydrogel/3D⁃printed poly（ε⁃caprolactone） hybrid scaffold containing synovial mesenchymal stem cells for cartilage regeneration based on tetrahedral framework nucleic acid recruitment［J］. Biomaterials, 2021,278:121131. doi: 10.1016/j.biomaterials.2021.121131.
［22］	Mai Z, Liu Q, Bian Y, et al. PCL/Collagen/UA composite biomedical dressing with ordered microfiberous structure fabricated by a 3D near⁃field electrospinning process［J］. Polymers （Basel）, 2022,15（1）:223. doi: 10.3390/polym15010223.
［23］	Vonbrunn E, Mueller M, Pichlsberger M, et al. Electrospun PCL/PLA scaffolds are more suitable carriers of placental mesenchymal stromal cells than collagen/elastin scaffolds and prevent wound contraction in a mouse model of wound healing［J］. Front Bioeng Biotechnol, 2020,8:604123. doi: 10.3389/fbioe.2020.604123.
［24］	Liu D, Chen S, Win Naing M. A review of manufacturing capabilities of cell spheroid generation technologies and future development［J］. Biotechnol Bioeng, 2021,118（2）:542⁃554. doi: 10.1002/bit.27620.
［25］	Alblawi A, Ranjani AS, Yasmin H, et al. Scaffold⁃free: a developing technique in field of tissue engineering［J］. Comput Methods Programs Biomed, 2020,185:105148. doi: 10.1016/j.cmpb.2019.105148.
［26］	Quashie D Jr, Benhal P, Chen Z, et al. Magnetic bio⁃hybrid micro actuators［J］. Nanoscale, 2022,14（12）:4364⁃4379. doi: 10.1039/d2nr00152g.
［27］	Fang Z, Lyu J, Li J, et al. Application of bioreactor technology for cell culture⁃based viral vaccine production: present status and future prospects［J］. Front Bioeng Biotechnol, 2022,10:921755. doi: 10.3389/fbioe.2022.921755.
［28］	Katt ME, Placone AL, Wong AD, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform［J］. Front Bioeng Biotechnol, 2016,4:12. doi: 10.3389/fbioe.2016.00012.
［29］	Wei W, Dai H. Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges［J］. Bioact Mater, 2021,6（12）:4830⁃4855. doi: 10.1016/j.bioactmat.2021.05.011.
［30］	Li F, Truong VX, Thissen H, et al. Microfluidic encapsulation of human mesenchymal stem cells for articular cartilage tissue regeneration［J］. ACS Appl Mater Interfaces, 2017,9（10）:8589⁃8601. doi: 10.1021/acsami.7b00728.
［31］	Polidoro MA, Ferrari E, Marzorati S, et al. Experimental liver models: from cell culture techniques to microfluidic organs⁃on⁃chip［J］. Liver Int, 2021,41（8）:1744⁃1761. doi: 10.1111/liv. 14942.
［32］	Jo H, Brito S, Kwak BM, et al. Applications of mesenchymal stem cells in skin regeneration and rejuvenation［J］. Int J Mol Sci, 2021,22（5）:2410. doi: 10.3390/ijms22052410.
［33］	Li B, Luan S, Chen J, et al. The MSC⁃derived exosomal lncRNA H19 promotes wound healing in diabetic foot ulcers by upregulating PTEN via microRNA⁃152⁃3p［J］. Mol Ther Nucleic Acids, 2020,19:814⁃826. doi: 10.1016/j.omtn.2019.11.034.
［34］	Guillamat⁃Prats R. The role of MSC in wound healing, scarring and regeneration［J］. Cells, 2021,10（7）:1729. doi: 10.3390/cells10071729.
［35］	Kouroupis D, Correa D. Increased mesenchymal stem cell functionalization in three⁃dimensional manufacturing settings for enhanced therapeutic applications［J］. Front Bioeng Biotechnol, 2021,9:621748. doi: 10.3389/fbioe.2021.621748.
［36］	Yang J, Chen Z, Pan D, et al. Umbilical cord⁃derived mesenchymal stem cell⁃derived exosomes combined pluronic F127 hydrogel promote chronic diabetic wound healing and complete skin regeneration［J］. Int J Nanomedicine, 2020,15:5911⁃5926. doi: 10.2147/IJN.S249129.
［37］	Turner PR, McConnell M, Young SL, et al. 3D living dressing improves healing and modulates immune response in a thermal injury model［J］. Tissue Eng Part C Methods, 2022,28（8）:431⁃439. doi: 10.1089/ten.TEC.2022.0088.
［38］	Kim MH, Wu WH, Choi JH, et al. Conditioned medium from the three⁃dimensional culture of human umbilical cord perivascular cells accelerate the migration and proliferation of human keratinocyte and fibroblast［J］. J Biomater Sci Polym Ed, 2018,29（7⁃9）:1066⁃1080. doi: 10.1080/09205063.2017.1340045.
［39］	Li L, Ngo H, Hwang E, et al. Conditioned medium from human adipose⁃derived mesenchymal stem cell culture prevents UVB⁃induced skin aging in human keratinocytes and dermal fibroblasts［J］. Int J Mol Sci, 2019,21（1）:49. doi: 10.3390/ijms 21010049.
［40］	Deng M, Yu TZ, Li D, et al. Human umbilical cord mesenchymal stem cell⁃derived and dermal fibroblast⁃derived extracellular vesicles protect dermal fibroblasts from ultraviolet radiation⁃induced photoaging in vitro［J］. Photochem Photobiol Sci, 2020,19（3）:406⁃414. doi: 10.1039/c9pp00421a.
［41］	Hu S, Li Z, Cores J, et al. Needle⁃free injection of exosomes derived from human dermal fibroblast spheroids ameliorates skin photoaging［J］. ACS Nano, 2019,13（10）:11273⁃11282. doi: 10.1021/acsnano.9b04384.
［42］	Li A, Guo F, Pan Q, et al. Mesenchymal stem cell therapy: hope for patients with systemic lupus erythematosus［J］. Front Immunol, 2021,12:728190. doi: 10.3389/fimmu.2021.728190.
［43］	Yang C, Sun J, Tian Y, et al. Immunomodulatory effect of MSCs and MSCs⁃derived extracellular vesicles in systemic lupus erythematosus［J］. Front Immunol, 2021,12:714832. doi: 10. 3389/fimmu.2021.714832.
［44］	Zhu L, Lin X, Zhi L, et al. Mesenchymal stem cells promote human melanocytes proliferation and resistance to apoptosis through PTEN pathway in vitiligo［J］. Stem Cell Res Ther, 2020,11（1）:26. doi: 10.1186/s13287⁃019⁃1543⁃z.

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间充质干细胞三维培养技术及其在皮肤病治疗中的应用进展

Three-dimensional culture techniques of mesenchymal stem cells and their application in the treatment of skin diseases

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