中华皮肤科杂志 ›› 2020, Vol. 53 ›› Issue (10): 781-786.doi: 10.35541/cjd.20200505

• 论著 • 上一篇    下一篇

代谢综合征相关基因多态性与蒙古族寻常型银屑病的相关性及与HLA-C*06：02交互作用研究

黄艳平,吕新翔,孙志强,李欣,丁文媛,韩建文   

  1. 内蒙古医科大学附属医院皮肤科，呼和浩特  010010
  • 收稿日期:2020-05-25 修回日期:2020-08-11 发布日期:2020-09-30
  • 通讯作者: 韩建文 E-mail:hanjianwen1981@hotmail.com
  • 基金资助:
    国家自然科学基金（81660513）；内蒙古自治区自然科学基金（2018MS08030）；内蒙古自治区科技计划项目（2019GG082）；内蒙古医科大学青年创新基金（YKD2018QNCX068）

Relationship of polymorphisms in metabolic syndrome-related genes with psoriasis vulgaris and their interaction with HLA-C*06:02 in populations of Mongolian nationality

Huang Yanping, Lyu Xinxiang, Sun Zhiqiang, Li Xin, Ding Wenyuan, Han Jianwen   

  1. Department of Dermatology, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010010, China
  • Received:2020-05-25 Revised:2020-08-11 Published:2020-09-30
  • Contact: Han Jianwen E-mail:hanjianwen1981@hotmail.com
  • Supported by:
    National Natural Science Foundation of China（81660513）; Natural Science Foundation of Inner Mongolia Autonomous Region of China （2018MS08030）; Inner Mongolia Science and Technology Plan Project（2019GG082）; Youth Innovation Fund of Inner Mongolia Medical University（YKD2018QNCX068）

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摘要： 【摘要】 目的 探讨代谢综合征相关基因多态性与蒙古族寻常型银屑病（PsV）的相关性及其与HLA-C*06：02的交互作用。方法 内蒙古医科大学附属医院2012年12月至2018年3月住院的379例蒙古族PsV患者及518例蒙古族健康对照。选择16个既往报道的与代谢综合征及其包含疾病相关的单核苷酸多态性（SNP）及HLA-C*06：02，利用二代测序法和聚合酶链反应-序列特异性引物（PCR-SSP）对受试者进行基因分型。计算蒙古族PsV组及对照组16个变异位点及HLA-C*06：02的最小等位基因频率，χ2检验比较两组各SNP位点的最小等位基因频率差异。结果 蒙古族PsV患者中16个代谢综合征易感SNP位点最小等位基因频率与健康对照比较，差异无统计学意义（均P > 0.05），而HLA-C*06：02位点的最小等位基因频率差异有统计学意义（P = 4.09 × 10-35，OR = 3.41）。HLA-C* 06：02阳性受试者中，252例PsV患者的rs7593730_T和rs6931514_G最小等位基因频率与191例健康对照相比差异有统计学意义（P = 0.016，OR = 0.64；P = 0.041，OR = 1.33）；而在HLA-C*06：02阴性受试者中，两组间16个SNP位点的最小等位基因频率差异均无统计学意义（P > 0.05）。结论 rs7593730和rs6931514与内蒙古地区蒙古族寻常型银屑病可能相关，与HLA-C*06：02可能存在交互作用。

关键词: 银屑病, 代谢疾病, 多态性, 单核苷酸, 蒙古族, HLA?C*06：02

Abstract: 【Abstract】 Objective To investigate the relationship of polymorphisms in metabolic syndrome-related genes with psoriasis vulgaris （PsV） and their interaction with HLA-C*06:02 in populations of Mongolian nationality. Methods Totally, 379 PsV inpatients of Mongolian nationality and 518 healthy controls of Mongolian nationality were collected from the Affiliated Hospital of Inner Mongolia Medical University from December 2012 to March 2018. Sixteen previously reported single nucleotide polymorphisms （SNPs） and HLA-C*06:02, which were related to metabolic syndrome and its components, were selected. Next-generation sequencing and polymerase chain reaction-sequence specific primer （PCR-SSP） typing were performed to determine the genotypes of these subjects. Minor allele frequencies of the 16 mutation sites and HLA-C*06:02 were calculated in the PsV group and control group, and chi-square test was used to analyze the differences in the minor allele frequencies of SNPs between the 2 groups. Results There was no significant difference in the minor allele frequencies of the 16 SNPs susceptible to metabolic syndrome between the Mongolian PsV patients and healthy controls （all P > 0.05）, while the minor allele frequency of HLA-C*06:02 significantly differed between the 2 groups （P = 4.09 × 10-35, OR = 3.41）. Among the HLA-C*06:02-positive subjects, the minor allele frequencies of rs7593730_T and rs6931514_G significantly differed between the 252 PsV patients and 191 healthy controls （P = 0.016, OR = 0.64; P = 0.041, OR = 1.33, respectively）; no significant difference was observed in the minor allele frequencies of the 16 SNPs between the PsV patients and healthy controls among the HLA-C*06:02-negative subjects （P > 0.05）. Conclusion The SNPs of rs7593730 and rs6931514 may be related to PsV in populations of Mongolian nationality in Inner Mongolia, and may interact with HLA-C*06:02.

Key words: Psoriasis, Metabolic diseases, Polymorphism, single nucleotide, Mongolian, HLA?C*06:02