强脉冲光照射对小鼠皮肤胶原纤维、弹性纤维及透明质酸含量的影响

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Abstract:

Objective To investigate the effects of intense pulsed light （IPL） irradiation on the content of collagen fibers, elastic fibers and hyaluronic acid in Kunming mouse skin. Methods The dorsal skin of mice was divided into two areas: the right area was irradiated with IPL, and the left remaining unirradiated served as the control. Skin specimens were taken from the back of mice on day 1, 3, week 1, 2, 4 and 8 after the irradiation and subjected to staining with HE, sirius red and Gomori aldehyde-fuchsin for examinations of histological changes, type I and Ⅲ collagen fibers and elastic fibers. The hydroxyproline and hyaluronic acid content in skin tissues of mice was determined with ultraviolet spectrophotometry and radioimmunoassay respectively. Results After irradiation, a significant increase was observed in dermal thickness on week 2 （t = 4.623, P < 0.05）, 4, and 8 （t = 3.904, P < 0.05）, in type Ⅲ collagen fiber （t = 5.129, P < 0.05） on week 1, in type I and Ⅲ collagen fibers on week 2, 4 and 8 （both P < 0.05）, in elastic fibers from week 2 to 8 （P < 0.05）, and in hydroxyproline content from week 1 to 8 （all P < 0.05） in the skin of mice compared with unirradiated mice. In detail, dermal thickness increased by 18.71% on week 4, and type Ⅲ collagen fiber by 40.54% in irradiated mice compared with unirradiated mice. Further more, the hyaluronic acid content was elevated from day 1 to 3, but gradually declined from week 1 to 8, and remained statistically higher from day 1 to week 8 （P < 0.05） in irradiated mice compared to unirradiated mice. Conclusion IPL irradiation could induce an increase in the content of collagen fiber, elastic fiber and hyaluronic acid in the dorsal skin of mice.

Key words: Intense pulsed light (IPL), Collagen, Elastic fibrin, Hydroxyproline, Hyaluronic acid

References

[1] 卢忠. 皮肤激光医学与美容[M]. 上海: 复旦大学出版社, 2008: 134. [2] Bitter PH. Noninvasive rejuvenation of photodamaged skin using serial, full-face intense pulsed light treatments [J]. Dermatol Surg, 2000, 26(9): 835- 842. [3] Negishi K, Tezuka Y, Kushikata N, et al. Photorejuvenation for Asian skin by intense pulsed light [J]. Dermatol Surg, 2001, 27: 627-632. [4] Hernández-Pérez E, Ibiett EV. Gross and microscopie findings in patients submitted to nonablative full-face resurfacing using intense pulsed light: a preliminary study [J]. Dermatol Surg, 2002, 28: 651-655. [5] 邓向东, 李世荣. 强脉冲光照射兔皮肤后的组织学观察[J]. 中国美容医学, 2008, 17(1): 60-61. [6] Anderson RR, Parrish JA. Selective photothermolysis: Precise microsurgery by selective absorption of pulsed radiation [J]. Science, 1983, 220: 524-527. [7] Goldberg DJ. New collagen formation after dermal remodeling with an intense pulsed light source [J]. J cutan Laser Ther, 2000, 2: 59-61. [8] 王明利，柳大烈，袁强，等. 强脉冲光对大鼠皮肤胶原蛋白影响的定量分析[J]. 中国实用美容整形外科杂志, 2005, 16 (2) : 118-120. [9] 曹妍，吴迪，骆丹. 强脉冲光对BALB/c小鼠皮肤胶原的影响[J]. 中华皮肤科杂志, 2007, 40(12): 748-751. [10] Prieto VG, Diwan AH, Shea CR, et al. Effects of Intense Pulsed Light and the 1,064 nm Nd: YAG Laser on Sun-Damaged Human Skin: Histologic and Immunohistochemical Analysis [J]. Dermatologic Surgery, 2005, 31(5): 522-525. [11] DePalma RL, Krummel TM, Durham LA, et al. Characterization and quantitafion of wound matrix in fetal rabbit [J]. Matrix, 1989, 9: 224-231. [12] Longaker MT, Chiu ES, Adzick NS, et al. Studies in fetal woundhealing: prolonged presence of hyaluronic acid characterizes fetal wound fluid [J]. Ann Surg, 1991, 213: 292-296. [13] Toole BP. Developmental role of hyaluronate [J]. Connect Tissue Res, 1984, 10(1): 93-100. [14] Estes JM, Vande Berg JS, Adzick NS, et al. Phenotypic and functional features of myofibroblasts in sheep fetal wounds [J]. Differentiation, 1994, 56:173-181. [15] Park JC, Park BJ, Suh H, et al. Comparative study on motility of the cultured fetal and neonatal dermal fibroblasts in extracellular matrix [J].Yonsei Med J, 2001, 42 :587-594.

Effects of intense pulsed light irradiation on the content of collagen fibers, elastic fibers and hyaluronic acid in Kunming mouse skin

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