诱导性多功能干细胞在皮肤科研究与应用的进展

doi:10.3760/cma.j.issn.0412-4030.2019.07.001

Abstract

Abstract: 【Abstract】 Induced pluripotent stem cells （iPSCs） are a kind of stem cells induced from somatic cells by reprogramming with some specific pluripotent transcription factors. Compared with traditional stem cells, iPSCs have many advantages, including a wide range of sources, the ability of totipotent differentiation, and less ethical and sourcing issues associated with embryonic stem cells which have been debated for a long time. In dermatology, iPSCs can be used to make regenerative skin, establish models of skin diseases, provide a new platform for studying the pathogenesis of skin diseases, and perform cell treatment or gene modification treatment for specific skin diseases. At present, the application value of iPSCs has been verified among animal models, and clinical trials are also under way.

Key words: Multipotent stem cells, Regenerative medicine, Skin diseases, Disease models, animal, Gene therapy, Induced pluripotent stem cells

CLC Number:

R751.05

Li Yumei, Zhang Yixuan, Liu Liping. Induced pluripotent stem cells: advances in research and application in dermatology[J].Chinese Journal of Dermatology, 2019, 52(7): 445-449.

References ［27］

［1］	Takahashi K, Yamanaka S. A decade of transcription factor⁃mediated reprogramming to pluripotency［J］. Nat Rev Mol Cell Biol, 2016,17（3）:183⁃193. doi: 10.1038/nrm.2016.8.
［2］	Brix J, Zhou Y, Luo Y. The epigenetic reprogramming roadmap in generation of iPSCs from somatic cells［J］. J Genet Genomics, 2015,42（12）:661⁃670. doi: 10.1016/j.jgg.2015.10.001.
［3］	Teshigawara R, Cho J, Kameda M, et al. Mechanism of human somatic reprogramming to iPS cell［J］. Lab Invest, 2017,97（10）:1152⁃1157. doi: 10.1038/labinvest.2017.56.
［4］	Nishiguchi M, Kikuyama H, Kanazawa T, et al. Increases in iPS transcription factor （Oct4, Sox2, c⁃Myc, and Klf4） gene after modified electroconvulsive therapy［J］. Psychiatry Investig, 2015,12（4）:532⁃537. doi: 10.4306/pi.2015. 12.4.532.
［5］	Yang F, Wang N, Wang Y, et al. Activin⁃SMAD signaling is required for maintenance of porcine iPS cell self⁃renewal through upregulation of NANOG and OCT4 ［J］. J Cell Physiol, 2017,232（8）:2253⁃2262. doi: 10.1002/jcp.25747.
［6］	Hoang P, Wang J, Conklin BR, et al. Generation of spatial⁃patterned early⁃developing cardiac organoids using human pluripotent stem cells［J］. Nat Protoc, 2018,13（4）:723⁃737. doi: 10.1038/nprot.2018.006.
［7］	Osaki T, SGM U, Kamm RD. Microphysiological 3D model of amyotrophic lateral sclerosis （ALS） from human iPS⁃derived muscle cells and optogenetic motor neurons［J］. Sci Adv, 2018,4（10）:eaat5847. doi: 10.1126/sciadv.aat5847.
［8］	Ishida Y, Kawakami H, Kitajima H, et al. Vulnerability of Purkinje cells generated from spinocerebellar ataxia type 6 patient⁃derived iPSCs［J］. Cell Rep, 2016,17（6）:1482⁃1490. doi: 10.1016/j.celrep.2016.10.026.
［9］	Mandai M, Watanabe A, Kurimoto Y, et al. Autologous induced stem⁃cell⁃derived retinal cells for macular degeneration［J］. N Engl J Med, 2017,376（11）:1038⁃1046. doi: 10.1056/NEJMoa 1608368.
［10］	Gledhill K, Guo Z, Umegaki⁃Arao N, et al. Melanin transfer in human 3D skin equivalents generated exclusively from induced pluripotent stem cells［J/OL］. PLoS One, 2015,10（8）:e0136713. ［2018⁃08⁃03］. https://journals.plos.org/plosone/article?id=10.1371/ journal.pone.0136713. doi: 10.1371/journal.pone.0136713.
［11］	Yang R, Zheng Y, Burrows M, et al. Generation of folliculogenic human epithelial stem cells from induced pluripotent stem cells［J］. Nat Commun, 2014,5:3071. doi: 10.1038/ncomms4071.
［12］	Kim Y, Park N, Rim YA, et al. Establishment of a complex skin structure via layered co⁃culture of keratinocytes and fibroblasts derived from induced pluripotent stem cells［J］. Stem Cell Res Ther, 2018,9（1）:217. doi: 10.1186/s13287⁃018⁃0958⁃2.
［13］	Umegaki⁃Arao N, Pasmooij AM, Itoh M, et al. Induced pluripotent stem cells from human revertant keratinocytes for the treatment of epidermolysis bullosa［J］. Sci Transl Med, 2014,6（264）:264ra164. doi: 10.1126/scitranslmed.3009342.
［14］	Koehler KR, Hashino E. 3D mouse embryonic stem cell culture for generating inner ear organoids［J］. Nat Protoc, 2014,9（6）:1229⁃1244. doi: 10.1038/nprot.2014.100.
［15］	Lee J, Böscke R, Tang PC, et al. Hair follicle development in mouse pluripotent stem cell⁃derived skin organoids［J］. Cell Rep, 2018,22（1）:242⁃254. doi: 10.1016/j.celrep.2017.12.007.
［16］	Takagi R, Ishimaru J, Sugawara A, et al. Bioengineering a 3D integumentary organ system from iPS cells using an in vivo transplantation model［J］. Sci Adv, 2016,2（4）:e1500887. doi: 10.1126/sciadv.1500887.
［17］	Itoh M, Umegaki⁃Arao N, Guo Z, et al. Generation of 3D skin equivalents fully reconstituted from human induced pluripotent stem cells （iPSCs）［J/OL］. PLoS One, 2013,8（10）:e77673. ［2018⁃08⁃03］. https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077673. doi: 10. 1371/journal.pone.0077673.
［18］	Matsumura W, Fujita Y, Nakayama C, et al. Establishment of integration⁃free induced pluripotent stem cells from human recessive dystrophic epidermolysis bullosa keratinocytes［J］. J Dermatol Sci, 2018,89（3）:263⁃271. doi: 10.1016/j.jdermsci.2017. 11.017.
［19］	Shalom⁃Feuerstein R, Serror L, Aberdam E, et al. Impaired epithelial differentiation of induced pluripotent stem cells from ectodermal dysplasia⁃related patients is rescued by the small compound APR⁃246/PRIMA⁃1MET［J］. Proc Natl Acad Sci U S A, 2013,110（6）:2152⁃2156. doi: 10.1073/pnas.1201753109.
［20］	El⁃Darouti M, Fawzy M, Amin I, et al. Treatment of dystrophic epidermolysis bullosa with bone marrow non⁃hematopoeitic stem cells: a randomized controlled trial［J］. Dermatol Ther, 2016,29（2）:96⁃100. doi: 10.1111/dth.12305.
［21］	Iinuma S, Aikawa E, Tamai K, et al. Transplanted bone marrow⁃derived circulating PDGFRα+ cells restore type Ⅶ collagen in recessive dystrophic epidermolysis bullosa mouse skin graft［J］. J Immunol, 2015,194（4）:1996⁃2003. doi: 10.4049/jimmunol. 1400914.
［22］	Nakayama C, Fujita Y, Matsumura W, et al. The development of induced pluripotent stem cell⁃derived mesenchymal stem/stromal cells from normal human and RDEB epidermal keratinocytes［J］. J Dermatol Sci, 2018,91（3）:301⁃310. doi: 10. 1016/j.jdermsci.2018.06.004.
［23］	Tolar J, McGrath JA, Xia L, et al. Patient⁃specific naturally gene⁃reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa［J］. J Invest Dermatol, 2014,134（5）:1246⁃1254. doi: 10.1038/jid.2013.523.
［24］	Wenzel D, Bayerl J, Nyström A, et al. Genetically corrected iPSCs as cell therapy for recessive dystrophic epidermolysis bullosa［J］. Sci Transl Med, 2014,6（264）:264ra165. doi: 10.1126/ scitranslmed.3010083.
［25］	Sebastiano V, Zhen HH, Haddad B, et al. Human COL7A1⁃corrected induced pluripotent stem cells for the treatment of recessive dystrophic epidermolysis bullosa［J］. Sci Transl Med, 2014,6（264）:264ra163. doi: 10.1126/scitranslmed.3009540.
［26］	Shinkuma S, Guo Z, Christiano AM. Site⁃specific genome editing for correction of induced pluripotent stem cells derived from dominant dystrophic epidermolysis bullosa［J］. Proc Natl Acad Sci U S A, 2016,113（20）:5676⁃5681. doi: 10.1073/pnas.15120 28113.
［27］	Nguyen HV, Li Y, Tsang SH. Patient⁃specific iPSC⁃derived RPE for modeling of retinal diseases［J］. J Clin Med, 2015,4（4）:567⁃578. doi: 10.3390/jcm4040567.

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[14]	XIONG Lin, RAN Yuping, ZHOU Guangping. Study on Taxonomy of the Genus Malassezia and the Distribution of it in Some Dermatoses [J]. Chinese Journal of Dermatology, 2000, 33(5): 318-320.
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Induced pluripotent stem cells: advances in research and application in dermatology

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