脂肪干细胞基质胶临床应用进展

doi:10.35541/cjd.20240300

Abstract

Abstract: 【Abstract】 Stromal vascular fraction gel （SVF-gel） is a highly active substance obtained by mechanically cutting and centrifugation of adipose tissues, with mature fat cells and oil droplets removed in the process. Due to the reserve of adipose-derived stem cells, extracellular matrix, and concentrated stromal vascular fractions, as well as the capability for low-temperature storage, SVF-gel has been widely used in various fields such as filling, improvement of pigmentation abnormalities, scar treatment, and wound repair in recent years. This review summarizes the current status and prospects of the application of SVF-gel in burn care, plastic surgery, and dermatology.

Key words: Stem cells, Dermal fillers, Stromal vascular fraction gel, Adipose-derived stem cells, Pigmentation disorder, Wound healing

Yuan Mengsha, Zhao Tao, Liu Ling. Clinical application of stromal vascular fraction gel[J]. Chinese Journal of Dermatology,2025,e20240300. doi:10.35541/cjd.20240300

References ［44］

［1］	Dong Z, Peng Z, Chang Q, et al. The survival condition and immunoregulatory function of adipose stromal vascular fraction （SVF） in the early stage of nonvascularized adipose transplantation［J/OL］. PLoS One, 2013,8（11）:e80364. doi: 10. 1371/journal.pone.0080364.
［2］	Tonnard P, Verpaele A, Peeters G, et al. Nanofat grafting: basic research and clinical applications［J］. Plast Reconstr Surg, 2013,132（4）:1017⁃1026. doi: 10.1097/PRS.0b013e31829fe1b0.
［3］	Yao Y, Dong Z, Liao Y, et al. Adipose extracellular matrix/stromal vascular fraction gel: a novel adipose tissue⁃derived injectable for stem cell therapy［J］. Plast Reconstr Surg, 2017,139（4）:867⁃879. doi: 10.1097/PRS.0000000000003214.
［4］	Jiang W, Wang J, Lin J, et al. Adipose⁃derived stem cell⁃enriched lipotransfer reverses skin sclerosis by suppressing dermal inflammation［J］. Plast Reconstr Surg, 2022,150（3）:578⁃587. doi: 10.1097/PRS.0000000000009435.
［5］	Liu J, Wang J, Zhang Q, et al. Clinical, histologic, and transcriptomic evaluation of sequential fat grafting for morphea: a nonrandomized controlled trial［J］. JAMA Dermatol, 2024,160（4）:425⁃433. doi: 10.1001/jamadermatol.2023.5908.
［6］	Cao Z, Li H, Wang ZH, et al. High⁃density fat grafting assisted stromal vascular fraction gel in facial deformities［J］. J Craniofac Surg, 2022,33（1）:108⁃111. doi: 10.1097/SCS.000000000000 8038.
［7］	侯崇超, 周传德. SVF⁃gel联合自体颗粒脂肪在外生殖器整形中的临床应用［J］. 中国美容整形外科杂志, 2019,30（3）:179⁃181,后插3. doi: 10.3969/j.issn.1673⁃7040.2019.03.017.
［8］	Li M, Tian Y, Cheng R, et al. Clinical efficacy of stromal vascular fraction gel in the treatment of mature striae distensae［J］. Skin Res Technol, 2024,30（1）:e13551. doi: 10.1111/srt. 13551.
［9］	Zhang Y, Zou J, Yuan Y, et al. Contouring and augmentation of the temple using stromal vascular fraction gel grafting［J］. Front Surg, 2022,9:893219. doi: 10.3389/fsurg.2022.893219.
［10］	Sun Y, Wu G, Dai T, et al. Stromal vascular fraction gel （SVF⁃Gel） combined with nanofat for tear trough deformity［J］. Aesthetic Plast Surg, 2024,48（2）:213⁃220. doi: 10.1007/s00266⁃023⁃03698⁃0.
［11］	Cai J, Wang J, Hu W, et al. Mechanical micronization of lipoaspirates for the treatment of horizontal neck lines［J］. Plast Reconstr Surg, 2020,145（2）:345⁃353. doi: 10.1097/PRS.00000 00000006456.
［12］	Yang RL, Chen SY, Fu SP, et al. Antioxidant mechanisms of mesenchymal stem cells and their therapeutic potential in vitiligo［J］. Front Cell Dev Biol, 2023,11:1293101. doi: 10.3389/fcell. 2023.1293101.
［13］	Zavala G, Sandoval C, Meza D, et al. Differentiation of adipose⁃derived stem cells to functional CD105（neg） CD73（low） melanocyte precursors guided by defined culture condition［J］. Stem Cell Res Ther, 2019,10（1）:249. doi: 10.1186/s13287⁃019⁃1364⁃0.
［14］	Bian Y, Yu H, Jin M, et al. Repigmentation by combined narrow‑band ultraviolet B/adipose‑derived stem cell transplantation in the mouse model: role of Nrf2/HO‑1‑mediated Ca2+ homeostasis［J］. Mol Med Rep, 2022,25（1）:6 ［pii］. doi: 10.3892/mmr.2021.12522.
［15］	Wang S, Wang P, Zhang R. Adipose tissue⁃derived Muse cells promote autophagy and oxidative stress tolerance in human epidermal melanocytes［J］. Cell Tissue Bank, 2023,24（1）:253⁃264. doi: 10.1007/s10561⁃022⁃10031⁃7.
［16］	Refat MA, Strassner JP, Frisoli ML, et al. Lesional CD8+ T⁃cell number predicts surgical outcomes of melanocyte⁃keratinocyte transplantation surgery for vitiligo［J］. J Invest Dermatol, 2023,143（11）:2275⁃2282.e6. doi: 10.1016/j.jid.2023.03.1689.
［17］	Symonds E, Black B, Brown A, et al. Adipose derived stem cell extracellular vesicles modulate primary human macrophages to an anti⁃inflammatory phenotype in vitro［J］. J Extracell Biol, 2023,2（8）:e104. doi: 10.1002/jex2.104.
［18］	Lim WS, Kim CH, Kim JY, et al. Adipose⁃derived stem cells improve efficacy of melanocyte transplantation in animal skin［J］. Biomol Ther （Seoul）, 2014,22（4）:328⁃333. doi: 10.4062/biomolther.2014.065.
［19］	Wang XY, Guan XH, Yu ZP, et al. Human amniotic stem cells⁃derived exosmal miR⁃181a⁃5p and miR⁃199a inhibit melanogenesis and promote melanosome degradation in skin hyperpigmentation, respectively［J］. Stem Cell Res Ther, 2021,12（1）:501. doi: 10.1186/s13287⁃021⁃02570⁃9.
［20］	Shen JP, Wu YX, Tang SJ, et al. Experimental study on stromal vascular fraction mediated inhibition of skin pigmentation in guinea pigs［J］. Ann Transl Med, 2022,10（23）:1268. doi: 10. 21037/atm⁃22⁃5433.
［21］	Zhao H, Hao L, Chen X, et al. An efficacy study of a new radical treatment for acne vulgaris using fat injection［J］. Aesthet Surg J, 2021,41（8）:NP1061⁃NP1072. doi: 10.1093/asj/sjab162.
［22］	Xiao S, Qi J, Li J, et al. Mechanical micronization of lipoaspirates combined with fractional CO2 laser for the treatment of hypertrophic scars［J］. Plast Reconstr Surg, 2023,151（3）:549⁃559. doi: 10.1097/PRS.0000000000009915.
［23］	朱萌莹, 陈龙金, 张明军, 等. 脂肪细胞外基质凝胶（SVF⁃gel）促进增生性瘢痕重塑的临床研究［J］. 中国医疗美容, 2022,12（11）:39⁃42. doi: 10.19593/j.issn.2095⁃0721.2022.11.011.
［24］	邓红柳, 冯倩. SVF⁃gel与超脉冲CO2点阵激光治疗凹陷性痤疮瘢痕的对比研究［J］. 检验医学与临床, 2021,18（2）:249⁃251. doi: 10.3969/j.issn.1672⁃9455.2021.02.031.
［25］	Cai Y, Zhang F, Feng J, et al. Long⁃term follow⁃up and exploration of the mechanism of stromal vascular fraction gel in chronic wounds［J］. Stem Cell Res Ther, 2023,14（1）:163. doi: 10.1186/s13287⁃023⁃03389⁃2.
［26］	Deng C, Wang L, Feng J, et al. Treatment of human chronic wounds with autologous extracellular matrix/stromal vascular fraction gel: a STROBE⁃compliant study［J］. Medicine （Baltimore）, 2018,97（32）:e11667. doi: 10.1097/MD.0000000 000011667.
［27］	Ma J, Zhang Z, Wang Y, et al. Investigation of miR⁃126⁃3p loaded on adipose stem cell⁃derived exosomes for wound healing of full⁃thickness skin defects［J］. Exp Dermatol, 2022,31（3）:362⁃374. doi: 10.1111/exd.14480.
［28］	Ren S, Chen J, Guo J, et al. Exosomes from adipose stem cells promote diabetic wound healing through the eHSP90/LRP1/AKT axis［J］. Cells, 2022,11（20）:3229. doi: 10.3390/cells11203229.
［29］	Liang Q, Zhou D, Ge X, et al. Exosomes from adipose⁃derived mesenchymal stem cell improve diabetic wound healing and inhibit fibrosis via miR⁃128⁃1⁃5p/TGF⁃β1/Smad axis［J］. Mol Cell Endocrinol, 2024,588:112213. doi: 10.1016/j.mce.2024. 112213.
［30］	Ren H, Su P, Zhao F, et al. Adipose mesenchymal stem cell⁃derived exosomes promote skin wound healing in diabetic mice by regulating epidermal autophagy［J］. Burns Trauma, 2024,12:tkae001. doi: 10.1093/burnst/tkae001.
［31］	Zhang T. Hepatocyte growth factor⁃modified adipose⁃derived mesenchymal stem cells inhibit human hypertrophic scar fibroblast activation［J］. J Cosmet Dermatol, 2024. doi: 10.1111/jocd.16509.
［32］	Yang J, Li S, He L, et al. Adipose⁃derived stem cells inhibit dermal fibroblast growth and induce apoptosis in keloids through the arachidonic acid⁃derived cyclooxygenase⁃2/prostaglandin E2 cascade by paracrine［J］. Burns Trauma, 2021,9:tkab020. doi: 10.1093/burnst/tkab020.
［33］	Chen S, He Z, Xu J. Application of adipose⁃derived stem cells in photoaging: basic science and literature review［J］. Stem Cell Res Ther, 2020,11（1）:491. doi: 10.1186/s13287⁃020⁃01994⁃z.
［34］	Yang Z, Dong L, Jin S, et al. Comparison of microfat, nanofat, and extracellular matrix/stromal vascular fraction gel for skin rejuvenation: basic animal research［J］. Aesthet Surg J, 2023,43（7）:NP573⁃NP586. doi: 10.1093/asj/sjad058.
［35］	Legiawati L, Suseno LS, Sitohang I, et al. Combination of adipose⁃derived stem cell conditioned media and minoxidil for hair regrowth in male androgenetic alopecia: a randomized, double⁃blind clinical trial［J］. Stem Cell Res Ther, 2023,14（1）:210. doi: 10.1186/s13287⁃023⁃03440⁃2.
［36］	Tak YJ, Lee SY, Cho AR, et al. A randomized, double⁃blind, vehicle⁃controlled clinical study of hair regeneration using adipose⁃derived stem cell constituent extract in androgenetic alopecia［J］. Stem Cells Transl Med, 2020,9（8）:839⁃849. doi: 10.1002/sctm.19⁃0410.
［37］	Cho SB, Roh MR, Chung KY. Recovery of scleroderma⁃induced atrophic alopecia by autologous fat transplantation［J］. Dermatol Surg, 2010,36（12）:2061⁃2063. doi: 10.1111/j.1524⁃4725.2010. 01783.x.
［38］	Dini M, Mori A, Quattrini Li A. Eyebrow regrowth in patient with atrophic scarring alopecia treated with an autologous fat graft［J］. Dermatol Surg, 2014,40（8）:926⁃928. doi: 10.1111/DSU.000000 0000000051.
［39］	Vestita M, Filoni A, Bonamonte D, et al. Abstract: the use of nanofat in androgenic alopecia. A prospective blinded study［J］. Plast Reconstr Surg Glob Open, 2017,5（9 Suppl）:90. doi: 10.1097/01.GOX.0000526293.77976.7f.
［40］	Perez⁃Meza D, Ziering C, Sforza M, et al. Hair follicle growth by stromal vascular fraction⁃enhanced adipose transplantation in baldness［J］. Stem Cells Cloning, 2017,10:1⁃10. doi: 10.2147/SCCAA.S131431.
［41］	Morinaga H, Mohri Y, Grachtchouk M, et al. Obesity accelerates hair thinning by stem cell⁃centric converging mechanisms［J］. Nature, 2021,595（7866）:266⁃271. doi: 10.1038/s41586⁃021⁃03624⁃x.
［42］	Shukla L, Morrison WA, Shayan R. Adipose⁃derived stem cells in radiotherapy injury: a new frontier［J］. Front Surg, 2015,2:1. doi: 10.3389/fsurg.2015.00001.
［43］	Lin Z, Shibuya Y, Imai Y, et al. Therapeutic potential of adipose⁃derived stem cell⁃conditioned medium and extracellular vesicles in an in vitro radiation⁃induced skin injury model［J］. Int J Mol Sci, 2023,24（24）:17214. doi: 10.3390/ijms242417214.
［44］	Yao C, Zhou Y, Wang H, et al. Adipose⁃derived stem cells alleviate radiation⁃induced dermatitis by suppressing apoptosis and downregulating cathepsin F expression［J］. Stem Cell Res Ther, 2021,12（1）:447. doi: 10.1186/s13287⁃021⁃02516⁃1.

[1]	Xu Zhuohong, Hu Yu, Gu Heng. Role of mesenchymal stem cell-derived exosomes in skin diseases [J]. Chinese Journal of Dermatology, 2025, 0(3): 20220881-e20220881.
[2]	Wang Feng, Xia Rushan . Roles of melanocyte stem cells in hair follicles in hair graying [J]. Chinese Journal of Dermatology, 2025, 58(2): 182-185.
[3]	Guo Weinan, Wang Junxia, Chen Hui, Hao Junfeng, Li Bing, Wei Jingyi, Zhao Tao. Clinical efficacy of autologous adipose-derived stem cell gel grafting in the treatment of depressed acne scars [J]. Chinese Journal of Dermatology, 2025, 58(2): 167-169.
[4]	Hua You, Ren Haiyan, Song Xiuzu. Dermoscopic and reflectance confocal microscopic characteristics of five cases of erythema dyschromicum perstans [J]. Chinese Journal of Dermatology, 2024, 57(9): 830-832.
[5]	Zhang Jiaqi, Wu Fan, Han Yuqing, Liu Qi, Pan Yao. Application of multi-photon microscopy in dermatology [J]. Chinese Journal of Dermatology, 2024, 57(9): 857-862.
[6]	Zhang Wei, Mu Xingyu, Hang Qianru, Huang Yijie, Xu Tengjiao, He Xiaojie, Ding Yan. Role of group 3 innate lymphoid cells in skin wound healing and underlying mechanisms [J]. Chinese Journal of Dermatology, 2024, 57(6): 516-523.
[7]	Zhang Yude, Wang Hongjuan, Kang Xiaojing. Stem cell therapy for vitiligo: advances in basic and clinical research [J]. Chinese Journal of Dermatology, 2024, 0(3): 20240127-e20240127.
[8]	Jiang Qian, Hu Bin, Chen Yao, Chen Liuqing. Acquired facial hyperpigmented macules in children: a retrospective analysis of clinical and non-invasive skin imaging features in 131 cases [J]. Chinese Journal of Dermatology, 2024, 0(2): 20240088-e20240088.
[9]	Yan Na, Chen Ting, Geng Songmei, Li Zhengxiao, Liu Yan, Ma Yingxin, Tan Xuanfeng. Application of subcutaneous asymmetric tension reduction combined with dermal buried angular mattress suture in surgical treatment of benign pigmented facial lesions in infants and young children [J]. Chinese Journal of Dermatology, 2024, 57(1): 54-57.
[10]	Wang Junxia, Guo Weinan, Chen Hui, Hao Junfeng, Li Bing, Wei Jingyi, Zhao Tao. Efficacy of autologous fat grafting in the treatment of 40 cases of stable linear scleroderma: a retrospective analysis [J]. Chinese Journal of Dermatology, 2023, 56(8): 762-765.
[11]	Li Yue, Wu Jinyan, Yuan Ruoyue, Yang Quyang, Zhao Xiansheng, Zhu Ningwen. Cell therapy for hereditary epidermolysis bullosa [J]. Chinese Journal of Dermatology, 2023, 56(7): 698-702.
[12]	Sun Linbo, Xu Jiaosheng. Curry-Jones syndrome: the first case reported in China [J]. Chinese Journal of Dermatology, 2023, 56(7): 626-629.
[13]	Yu Ling, Dou Jinfa, Wang Jianbo, Zhang Shoumin. Gene mutation analysis in a Chinese pedigree with autosomal dominant Waardenburg syndrome [J]. Chinese Journal of Dermatology, 2023, 56(3): 241-243.
[14]	Chen Jincong, Lin Lihang, Su Huichun, . Roles of Langerhans cells in atopic dermatitis [J]. Chinese Journal of Dermatology, 2023, 0(2): 20220279-e20220279.
[15]	Wang Qi, Jian Zhe. Three-dimensional culture techniques of mesenchymal stem cells and their application in the treatment of skin diseases [J]. Chinese Journal of Dermatology, 2023, 0(2): 20230083-e20230083.

Clinical application of stromal vascular fraction gel

PDF

PDF (Mobile)

Knowledge

Abstract

Cite this article

share this article

References ［44］

Related Articles 15

Metrics

Comments

Recommended 10