中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (2): 87-88.

• 论著 • 上一篇    下一篇

银屑病与亚甲基四氢叶酸还原酶基因多态位点A677V关系的研究

杨维玲1, 王晓慧1, 郑淑云1, 孙智晶2, 王柏秋2, 傅松滨2   

  1. 1. 哈尔滨医科大学附属第一医院 150001;
    2. 哈尔滨医科大学生物遗传学研究室
  • 收稿日期:1998-01-19 修回日期:1998-08-17 出版日期:1999-04-15 发布日期:1999-04-15
  • 基金资助:
    黑龙江省科委自然基金

Study on the Relationship Between Psoriasis and the Polymorphic Site A677V of Methylenetetrahydrofolate Reductase Gene

YANG Weiling1, WANG Xiaohui1, ZHENG Shuyun1   

  1. Department of Dermatology, First Affiliated Hospital of Harbin Medical University, Harbin 150001
  • Received:1998-01-19 Revised:1998-08-17 Online:1999-04-15 Published:1999-04-15

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摘要： 目的 探讨银屑病与亚甲基四氢叶酸还原酶基因多态位点A677V的关系.方法 采用聚合酶链反应-限制性片段长度多态性,检测了3个银屑病家系和39例银屑病患者的亚甲基四氢叶酸还原酶基因多态位点A677V.结果银屑病患者亚甲基四氢叶酸还原酶不耐热缺陷与正常人有显着性差异.结论 亚甲基四氢叶酸还原酶A677V突变型与银屑病发病相关.

关键词: 银屑病, 四氢叶酸脱氢酶, 聚合酶链反应-限制性片段长度多态

Abstract: Objective To investigate the relationship between polymorphic site A667V of 5,10-methylenetetrahydrofolate reductase (MTHFR) gene and psoriasis. Methods PCR-RFLP method was used to detect the polymorphism of MTHFR gene in 3 psoriasis pedigrees and 39 psoriatics. Results Genotype frequencies of the psoriastics were A/A-20.51%, A/V=48.72%and V/V=30.76%;the allelic frequencies were A=0.4487 and V=0.5513. Conclusion There is a significant difference of MTHFR gene thermolabile defect between the psoriatics and the control, and our results demonstrate MTHFR gene is related to A667V mutant.

Key words: Psoriasis, Tetrahydrofolate dehydrogenase, PCR-RFLP