中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (12): 855-858.

• 论著 • 上一篇    下一篇

细胞外信号调节激酶在寻常性银屑病皮损中的表达

葛新红1,秦璟2,张晓鸣3,汪京峡1,喻楠3,夏莉4,施慧娟5,王建军5,王乐5,杨晶5   

  1. 1. 宁夏医学院附属医院皮肤科
    2. 宁夏医科大学附属医院皮肤科
    3. 银川宁夏医学院附属医院皮肤科
    4. 宁夏医学院附属医院
    5.
  • 收稿日期:2010-06-08 修回日期:2010-07-12 出版日期:2010-12-15 发布日期:2010-12-13
  • 通讯作者: 葛新红 E-mail:gexinhong.0101@163.com
  • 基金资助:

    宁夏自治区卫生厅研究计划项目;宁夏自然科学基金资助项目

Expression of extracellular signal-regulated kinase in lesions of psoriasis vulgaris

  • Received:2010-06-08 Revised:2010-07-12 Online:2010-12-15 Published:2010-12-13

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摘要：

目的 探讨细胞外信号调节激酶（ERK1/2）及其上游相关激酶表皮生长因子受体（EGFR）、下游作用底物激酶转录激活因子ETS样蛋白-1（ELK-1）在寻常性银屑病皮损中的表达、激活状况，探讨细胞外信号调节激酶通路与寻常性银屑病的关系。方法 用免疫组化及蛋白印迹的方法，检测磷酸化ERK1/2、EGFR、ELK-1在寻常性银屑病和正常人皮肤中的表达状况。结果 寻常性银屑病皮损中磷酸化ERK1/2、EGFR、ELK-1的表达（IOD值分别为269.85 ± 57.96、136.88 ± 30.33、237.61 ± 56.29）均高于正常人皮肤（分别为140.24 ± 24.42、110.66 ± 28.99和119.04 ± 21.99）（P均 < 0.05）。银屑病患者磷酸化EGFR与ERK1/2表达呈正相关（r = 0.57，P < 0.05），ERK1/2与ELK-1表达亦呈正相关（r = 0.72，P < 0.05）。结论 磷酸化EGFR→ERK1/2→ELK-1这条信号通路的传导增强可能在寻常性银屑病的病理生理过程中起一定的作用。

关键词: 银屑病, 细胞外信号调节MAP激酶类, 表皮生长因子受体, 转录激活因子类

Abstract:

Objective To investigate the expression and activation of extracellular signal-regulated kinase 1/2（ERK1/2）, its upstream molecule, epidermal growth factor receptor （EGFR）, and downstream transcription factor, Ets-like protein 1 （ELK-1）, in lesions of psoriasis vulgaris, and to evaluate the relationship between ERK pathway and psoriasis vulgaris. Methods Tissue samples were obtained from the lesions of 40 patients with psoriasis vulgaris and normal skin of 20 normal human controls. Immunohistochemistry and Western blot were performed to detect the expressions of phosphorylated ERK1/2, EGFR and ELK-1 in the tissue samples. Results As immunohistochemistry showed, the integrated optical density （IOD） of p-ERK1/2, p-EGFR and p-ELK-1 was 269.85 ± 57.96, 136.88 ± 30.33 and 237.61 ± 56.29 respectively in the psoriatic lesions, significantly higher than that in the normal controls （140.24 ± 24.42, 110.66 ± 28.99 and 119.04 ± 21.99, respectively, all P < 0.05）. A positive correlation was observed between the expression of p-EGFR and p-ERK1/2 （r = 0.57, P < 0.05） and between that of p-ERK1/2 and p-ELK-1 （r = 0.72, P < 0.05） in psoriatic lesions. Conclusion The enhanced signal transduction through phosphorylated EGFR→ERK1/2→ELK-1 pathway may play a certain role in the pathophysiological process of psoriasis vulgaris.

Key words: Psoriasis, Epidermal Growth Factor receptors, Activating Transcription Factors.