中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (5): 314-317.

• 论著 • 上一篇    下一篇

2004-2006年广东省性病实验室梅毒血清学检测能力分析

吴兴中 郑和平 黄进梅   

  1. 广东省皮肤性病防治中心 广州广东省皮肤病防治研究所 广州市广东省皮肤病防治中心
  • 收稿日期:2007-08-07 修回日期:2007-10-16 发布日期:2008-05-15
  • 通讯作者: 郑和平 E-mail:zhhpf@hotmail.com,hpzheng@21cn.com

Quality evaluation and proficiency testing of serological test of syphilis from 2004 to 2006 in Guangdong

xingzhong wu   

  • Received:2007-08-07 Revised:2007-10-16 Published:2008-05-15

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摘要： 目的 分析广东省性病实验室梅毒血清学检测能力，提高检测质量。方法 制备并发放梅毒血清样本，各性病实验室按照分级管理要求分别做非梅毒螺旋体和梅毒螺旋体血清学定性和定量试验，统计分析各实验室检测结果。 结果 2004-2006年间共计有225家性病实验室参评3696份梅毒血清学试验，全省总体符合率由2004年的82.5％上升到2006年的90.0％，检测能力显著增高（χ2 = 16.8，P < 0.05 ），操作水平得分在全省平均水平之上（SI≥0）的实验室比例由2004年的64.7％上升到2006年的76.8％（χ2 = 4.6，P < 0.05）。非梅毒螺旋体和梅毒螺旋体血清学定性试验的总体符合率分别为95.5％和95.0％，两者的假阴性率和假阳性率分别只有4.7％和4.0％、7.2％和0.4％。非梅毒螺旋体和梅毒螺旋体血清学定量试验总体符合率分别为82.1％和81.1％，符合率和SI≥0的实验室呈逐年增加趋势，几何标准差逐年减小（3.6-2.9和4.9-2.7）。结论 持续的梅毒实验室诊断能力室间评价，促进梅毒血清学检测能力的提高，对于梅毒的防治有重要意义。

关键词: 性传播疾病, 实验室, 梅毒, 血清学试验, 检测能力

Abstract: Objective To assess the performance capability of syphilis serology among STI laboratories in Guangdong Province. Methods Positive and negative sera samples were prepared and delivered to STI laboratories. Non-treponemal and treponemal quantitative and qualitative tests were performed with these samples based on standardized procedures. Quality and proficiency of syphilis serology among these laboratories were evaluated. Results A total of 225 STI laboratories in Guangdong Province participated in this survey, 3696 serological tests for syphilis were performed from 2004-2006. The outcomes showed that the total reproducibility among participating laboratories increased from 82.5% in 2004 to 90.0% in 2006 （χ2 = 16.8, P < 0.05）, and the percentage of laboratories with a performance level higher than the provincial average level rose from 64.7% in 2004 to 76.8% in 2006. The total reproducibility was 95.5% for non-treponemal qualitative tests，95.0% for treponemal qualitative tests, 82.1% for non-treponemal quantitative tests, and 81.1% for treponemal quantitative tests. The false-negativity and false-positivity rates were 4.7% and 4.0%, respectively, for non-treponemal qualitative tests, and 7.2% and 0.4%, respectively, for treponemal qualitative tests. Conclusions It is important to do capability building and improve the performance of STI laboratories, in order to prevent and control syphilis.

Key words: Sexually Transmitted Infections, Laboratories, Syphilis, Serological Test, Proficiency of Test