中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (1): 12-14.

• 论著 • 上一篇    下一篇

副肿瘤性天疱疮自身抗原表位研究

张秉新1,郑瑞2,王京3,等4   

  1. 1. 天津中医药大学第一附属医院皮肤科（联系人：朱学骏）
    2. 太原市山西医科大学附属第一医院皮肤科
    3. 深圳市第四人民医院
    4. 湖北省中山医院整形美容外科
  • 收稿日期:2007-03-30 修回日期:2007-06-19 发布日期:2008-01-31
  • 通讯作者: 张秉新 E-mail:zbingxin@163.com

  • Received:2007-03-30 Revised:2007-06-19 Published:2008-01-31

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摘要： 目的 探讨副肿瘤性天疱疮的自身抗原表位。方法 运用重组融合蛋白免疫印迹实验、合成短肽酶联免疫吸附实验、竞争性酶联免疫吸附实验和斑点免疫印迹实验对副肿瘤性天疱疮的主要自身抗原-斑蛋白家族分子进行研究。结果 斑蛋白家族中周斑蛋白和包斑蛋白的L亚区是副肿瘤性天疱疮患者血清识别的主要抗原，其中包斑蛋白L亚区第1738 ～ 1757位氨基酸序列中存在着特异性抗原表位，可被多数副肿瘤性天疱疮血清识别。结论 包斑蛋白L亚区中存在着副肿瘤性天疱疮的特异性抗原表位。

关键词: 天疱疮, 自身抗体, 斑蛋白类

Abstract: Objective To investigate the autoantigen epitopes in paraneoplastic pemphigus （PNP）. Methods Serum samples were collected from 12 patients with PNP, 12 with pemphigus vulgaris, 12 with pemphigus foliaceus, 12 with bullous pemphigus and 20 normal controls. Immunoblotting was performed using recombinant fused proteins including envoplakin, periplakin, desmoplakin and BPAG1. Twelve peptides covering the linker subdomain of envoplakin were synthesized for ELISA and dot blotting to delineate the epitopes in this region. Results The recombinant linker subdomains of envoplakin, periplakin, desmoplakin and BPAG1 were recognized by 11, 11, 7 and 9 of the 12 sera from PNP patients, respectively. As shown by ELISA, all the 12 peptides demonstrated different specificity and affinity to PNP sera，and most of the results were consistent between dot blotting and ELISA. There were specific antigen epitopes in the linker subdomain of envoplakin from site 1738 to 1757, which could be recognized by the sera from PNP patients. Conclusion Specific antigen epitopes of PNP are present in the linker subdomain of envoplakin.