中华皮肤科杂志 ›› 2022, Vol. 55 ›› Issue (3): 235-237.doi: 10.35541/cjd.20200848

• 研究报道 • 上一篇    下一篇

盐裂皮肤间接免疫荧光技术在大疱性类天疱疮诊断中的价值评估

李锁    向睿宇    李志量    荆可    王媛    张寒梅    冯素英   

  1. 中国医学科学院、北京协和医学院皮肤病医院皮肤科，南京  210042
  • 收稿日期:2020-08-26 修回日期:2021-02-06 发布日期:2022-03-03
  • 通讯作者: 冯素英 E-mail:fengsy@pumcderm.cams.cn
  • 基金资助:
    中国医学科学院医学与健康科技创新工程项目（CIFMS-2017-I2M-1-017）；南京市国家级临床医学中心培育计划项目（2019060001）；江苏省卫生健康委科研项目（ZD2021035）

Evaluation of the value of indirect immunofluorescence on salt-split skin in the diagnosis of bullous pemphigoid

Li Suo, Xiang Ruiyu, Li Zhiliang, Jing Ke, Wang Yuan, Zhang Hanmei, Feng Suying   

  1. Department of Dermatology, Hospital of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
  • Received:2020-08-26 Revised:2021-02-06 Published:2022-03-03
  • Contact: Feng Suying E-mail:fengsy@pumcderm.cams.cn
  • Supported by:
    CAMS Innovation Fund for Medical Sciences （CIFMS-2017-I2M-1-017）; Nanjing Incubation Program for National Clinical Research Center （2019060001）；Scientific Research Project of Jiangsu Provincial Health Commission （ZD2021035）

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摘要： 【摘要】 目的 评价盐裂皮肤间接免疫荧光（IIF-SSS）在大疱性类天疱疮（BP）诊断中的价值。方法 采用单中心临床回顾性研究。纳入2013年1月至2019年1月在中国医学科学院皮肤病医院就诊的初诊BP患者163例，对照组404例，包括天疱疮161例、湿疹67例、药疹26例、多形红斑23例、结节性痒疹18例等。于患者用药前采血，行IIF-SSS、BP180 NC16A ELISA及直接免疫荧光（DIF）检测，比较IIF-SSS在BP诊断中的价值。计量资料比较采用t检验及Mann-Whitney检验；计数资料比较采用χ2检验及Fisher精确检验法或McNemar test检验。结果 BP组IIF-SSS、BP180 NC16A ELISA、DIF阳性例数分别为160、153和127，对照组分别为0、18和26。IIF-SSS、BP180 NC16A ELISA、DIF诊断BP的敏感性分别为98.15%、93.86%、77.91%，特异性分别为100%、95.54%、93.56%。IIF-SSS和DIF诊断BP具有较强的一致性（Kappa系数＝0.767，P < 0.001）。结论 IIF-SSS诊断BP敏感性和特异性高，可作为BP诊断的常规方法。

关键词: 类天疱疮, 大疱性, 诊断, 荧光抗体技术, 酶联免疫吸附测定, 盐裂皮肤间接免疫荧光技术

Abstract: 【Abstract】 Objective To evaluate the value of indirect immunofluorescence on salt-split skin （IIF-SSS） in the diagnosis of bullous pemphigoid （BP）. Methods A single-center clinical retrospective study was conducted. Totally, 163 patients with newly diagnosed BP were collected from Hospital of Dermatology, Chinese Academy of Medical Sciences from January 2013 to January 2019, so were 404 controls, including 161 with pemphigus, 67 with eczema, 26 with drug eruption, 23 with erythema multiforme, 18 with prurigo nodularis, etc. Blood samples were collected before the treatment, and IIF-SSS, BP180 NC16A enzyme-linked immunosorbent assay （ELISA） and direct immunofluorescence （DIF） assay were performed to evaluate the value of IIF-SSS in the diagnosis of BP. Measurement data were compared by using t test and Mann-Whitney test, and enumeration data were compared by using chi-square test and Fisher′s exact test or McNemar test. Results The number of cases positive for IIF-SSS, BP180 NC16A ELISA and DIF assay was 160, 153 and 127 respectively in the BP group, and 0, 18 and 26 respectively in the control group. The sensitivities of IIF-SSS, BP180 NC16A ELISA and DIF assay for the diagnosis of BP were 98.15%, 93.86% and 77.91% respectively, and their specificities were 100%, 95.54% and 93.56% respectively. There was strong consistency in the diagnosis of BP between IIF-SSS and DIF （Kappa coefficient = 0.767, P < 0.001）. Conclusion IIF-SSS has relatively high sensitivity and specificity for the diagnosis of BP, and can serve as a routine method for diagnosing BP.

Key words: Pemphigoid, bullous, Diagnosis, Fluorescent antibody technique, Enzyme-linked immunosorbent assay, Indirect immunofluorescence on salt-split skin

引用本文

李锁 向睿宇 李志量 荆可 王媛 张寒梅 冯素英. 盐裂皮肤间接免疫荧光技术在大疱性类天疱疮诊断中的价值评估[J]. 中华皮肤科杂志, 2022,55(3):235-237. doi:10.35541/cjd.20200848

Li Suo, Xiang Ruiyu, Li Zhiliang, Jing Ke, Wang Yuan, Zhang Hanmei, Feng Suying. Evaluation of the value of indirect immunofluorescence on salt-split skin in the diagnosis of bullous pemphigoid[J]. Chinese Journal of Dermatology, 2022, 55(3): 235-237.doi:10.35541/cjd.20200848