中华皮肤科杂志 ›› 2019, Vol. 52 ›› Issue (9): 611-615.doi: 10.3760/cma.j.issn.0412?4030.2019.09.005

• 论著 • 上一篇    下一篇

表没食子儿茶素没食子酸酯对斑块状银屑病患者Th1/Th2免疫平衡的影响

付丹丹    胡华    张孟杰    李敏    李占国    田中伟   

  1. 新乡医学院第一附属医院皮肤性病科，河南卫辉  453100 
  • 收稿日期:2018-12-26 修回日期:2019-04-16 出版日期:2019-09-15 发布日期:2019-08-30
  • 通讯作者: 田中伟 E-mail:zhonwt@163.com
  • 基金资助:
    河南省卫生计生委科技创新人才“51282”工程项目（201632）

Effect of epigallocatechin gallate on Th1/Th2 immune balance in patients with plaque?type psoriasis

Fu Dandan , Hu Hua, Zhang Mengjie, Li Min, Li Zhanguo, Tian Zhongwei   

  1. Department of Dermatology and Venereology, The First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan, China
  • Received:2018-12-26 Revised:2019-04-16 Online:2019-09-15 Published:2019-08-30
  • Contact: Tian Zhongwei E-mail:zhonwt@163.com
  • Supported by:
    Science and Technology Innovation Talents Project of Henan Health and Family Planning Commission （201632）

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摘要： 【摘要】 目的 探讨表没食子儿茶素没食子酸酯（EGCG）对银屑病患者辅助性T细胞1（Th1）和Th2的影响。方法 选取33例寻常型斑块状银屑病患者，分离外周血单个核细胞（PBMC）。MTT法选择合适的药物浓度。流式细胞仪检测Th1和Th2细胞比例，ELISA测定细胞上清液中Th1细胞因子白细胞介素2（IL?2）和干扰素γ（IFNγ）、Th2细胞因子IL?4和IL?10含量，qRT?PCR检测Th1细胞转录因子T?bet和Th2细胞转录因子GATA3 mRNA表达。采用t检验进行统计学分析。结果 根据MTT实验结果，选择无毒性作用的60 μmol/L EGCG作为实验浓度处理PBMC。与不加EGCG的对照组相比，EGCG组Th1细胞减少（t = 3.43，P = 0.026），Th2细胞增加（t = 6.68，P = 0.026），Th1/Th2比例降低（P < 0.05）。EGCG组PBMC培养上清液中IL?2（824.45 ± 101.21 ng/L）和IFNγ（1623.62 ± 185.56 ng/L）显著低于对照组（IL?2：1568.32 ± 196.45 ng/L；IFNγ：3287.63 ± 235.54 ng/L），而IL?4（389.48 ± 46.63 ng/L）和IL?10（285.95 ± 53.28 ng/L）显著高于对照组（IL?4：225.38 ± 26.92 ng/L； IL?10：165.46 ± 32.25 ng/L）。EGCG组转录因子T?bet mRNA表达降低（t = 11.99，P < 0.001），而GATA3 mRNA表达升高（t = 18.62，P < 0.001）。结论 EGCG能够减少Th1细胞数量，抑制其转录及细胞因子分泌，同时增加Th2细胞数量，提高其转录及细胞因子分泌，从而调节Th1/Th2免疫失衡。

关键词: 银屑病, Th1细胞, Th2细胞, 干扰素γ?, 白细胞介素类, 转录因子, 表没食子儿茶素没食子酸酯

Abstract: 【Abstract】 Objective To evaluate the effect of epigallocatechin gallate （EGCG） on T helper cell 1 （Th1） and Th2 in psoriasis patients. Methods A total of 33 patients with plaque?type psoriasis vulgaris were enrolled, and peripheral blood mononuclear cells （PBMC） were isolated and cultured. The appropriate concentration of EGCG was determined by methyl thiazol tetrazolium （MTT） assay. PBMC at exponential growth phase were divided into 2 groups to be treated with EGCG （EGCG group） or not （control group） for 24 hours. Flow cytometry was performed to determine proportions of Th1 and Th2 cells, enzyme?linked immunosorbent assay （ELISA） to detect levels of Th1 （interleukin ［IL］?2, interferon ［IFN］?γ） and Th2 cytokines （IL?4, IL?10） in the cell culture supernatant, and real?time quantitative RCR （qRT?PCR） to determine the mRNA expression of T?bet （a Th1 transcription factor） and GATA3 （a Th2 transcription factor）. Statistical analysis was carried out by using t test. Results According to the MTT assay results, EGCG at a non?toxic concentration of 60 μmol/L was chosen for subsequent experiments. Compared with the control group, the EGCG group showed significantly decreased number of Th1 cells （t = 3.43, P = 0.026）, increased number of Th2 cells （t = 6.68, P = 0.026）, and decreased Th1/Th2 ratio （P < 0.05）. The levels of IL?2 and IFN?γ in the culture supernatant of PBMC were both significantly lower in the EGCG group （824.45 ± 101.21 ng/L, 1 623.62 ± 185.56 ng/L respectively） than in the control group （1 568.32 ± 196.45 ng/L, 3 287.63 ± 235.54 ng/L respectively）, while the levels of IL?4 and IL?10 were significantly higher in the EGCG group （389.48 ± 46.63 ng/L, 285.95 ± 53.28 ng/L respectively） than in the control group （225.38 ± 26.92 ng/L, 165.46 ± 32.25 ng/L respectively）. Compared with the control group, the EGCG group showed significantly decreased T?bet mRNA expression （t = 11.99, P < 0.001）, but increased GATA3 mRNA expression （t = 18.62, P < 0.001）. Conclusion EGCG can reduce the number of Th1 cells, inhibit the production of Th1 cytokines and transcription factors, and increase the number of Th2 cells and the production of Th2 cytokines and transcription factors, followed by the modulation of Th1/Th2 immune imbalance.

Key words: Psoriasis, Th1 cells, Th2 cells, Interferon-gamma, Interleukins, Transcription factor, Epigallocatechin gallate