中华皮肤科杂志 ›› 2018, Vol. 51 ›› Issue (10): 729-732.doi: 10.3760/cma.j.issn.0412-4030.2018.10.005

• 论著 • 上一篇    下一篇

银屑病皮损CD8αα+ T细胞表型鉴定

张洋洋,李冰,王刚   

  1. 710032西安，第四军医大学西京皮肤医院
  • 收稿日期:2018-04-25 修回日期:2018-06-20 出版日期:2018-10-15 发布日期:2018-10-03
  • 通讯作者: 王刚 E-mail:xjwgang@fmmu.edu.cn
  • 基金资助:
    国家临床重点专科建设项目；陕西省科技计划项目（2016LCZX-06）；陕西省科技统筹创新工程计划项目（2016KTZDSF02-05）；陕西省社会发展科技攻关项目（2016SF-374）

Phenotype identification of CD8αα+ T cells infiltrating psoriatic skin lesions

Zhang Yangyang, Li Bing, Wang Gang   

  1. Department of Dermatology, Xijing Hospital, Fourth Military Medical University, Xi′an 710032, China
  • Received:2018-04-25 Revised:2018-06-20 Online:2018-10-15 Published:2018-10-03
  • Contact: Wang Gang E-mail:xjwgang@fmmu.edu.cn
  • Supported by:
    National Clinical Key Subject Construction Project; Science and Technology Planning Project of Shaanxi Province of China （2016LCZX-06）; Science and Technology Coordination and Innovation Project of Shaanxi Province （2016KTZDSF02-05）; Shaanxi Provincial Science and Technology Project for Social Development （2016SF-374）

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摘要： 目的 探究银屑病皮损局部浸润的CD8+ T细胞表型及其在银屑病发病中的作用。方法 收集2017年1 - 12月第四军医大学西京皮肤医院明确诊断的8例进行期斑块状银屑病患者皮损，男女各4例，年龄24 ~ 50岁。8例健康对照皮肤来源于整形外科手术剩余皮肤，男女各4例，年龄23 ~ 46岁。采用免疫荧光技术检测皮损CD8+ T细胞的分布及亚群比例，鉴定其免疫学表型及炎症因子白细胞介素（IL）17A的表达。结果 8例银屑病皮损真、表皮均可见CD8+ T细胞浸润，其中CD8αα+ T细胞表型占88.48% ± 7.39%，8例健康人皮肤局部仅有个别CD8+ T细胞浸润，其中CD8αα+ T细胞表型占14.43% ± 13.14%，两组比较，t = 11.5，P < 0.01。银屑病皮损表皮CD8αα+ T细胞表达组织局部定植标志CD103，真皮CD8αα+ T细胞不表达CD103。银屑病皮损CD8αα+ T细胞表达CD45RA-CCR7-效应记忆性T细胞表型，不表达CD8+ 调节性T细胞标志Foxp3、CD25和CD122。银屑病皮损CD8αα+ T细胞中分泌IL?17A的细胞比例为24.85% ± 4.25%，对照组CD8αα+ T细胞不分泌IL?17A，两组差异有统计学意义（t = 5.853，P < 0.01）。结论 银屑病皮损浸润的CD8αα+ T细胞是效应记忆性T细胞，可能通过分泌IL?17A参与银屑病的发生发展。

关键词: 银屑病, CD8阳性T淋巴细胞, 免疫表型分型, 白细胞介素17, 荧光抗体技术

Abstract: Objective To identify the phenotype of CD8αα+ T cells locally infiltrating psoriatic skin lesions, and to investigate their role in the occurrence of psoriasis. Methods Skin lesions were obtained from 8 patients with confirmed plaque psoriasis in the progressive stage, who visited the Department of Dermatology in Xijing Hospital affiliated to the Fourth Military Medical University from January to December in 2017. Of the 8 patients, 4 were male and 4 were female, with ages ranging from 24 to 50 years. Normal skin tissues were obtained from discarded skin tissues of 8 healthy controls in plastic surgery. Of the 8 healthy controls, 4 were male and 4 were female, with ages ranged from 23 to 46 years. Immunofluorescence technique was used to investigate the distribution of CD8αα+ T cells, determine the proportion of CD8αα+ T cell subsets, identify the immunological phenotype of CD8αα+ T cells and measure the of interleukin-17A （IL-17A）. Results Infiltration of CD8+ T cells was observed in the dermis and epidermis of the 8 psoriatic skin lesions, and the proportion of CD8αα+ T cells was 88.48% ± 7.39%. However, only a few CD8+ T cells locally infiltrated the control skin tissues, and the proportion of CD8αα+ T cells was 14.43% ± 13.14%. There was a significant difference in the proportion of CD8αα+ T cells between the psoriatic skin lesions and control skin tissues （t = 11.5, P < 0.01）. CD8αα+ T cells in the psoriatic epidermis expressed CD103 （a marker of tissue-resident cells）, while CD8αα+ T cells in the psoriatic dermis did not express CD103. CD8αα+ T cells in the psoriatic lesions were identified as CD45RA-CCR7- effector T cells, and did not express Foxp3, CD25 and CD122 （markers of CD8+ regulatory T cells）. The proportion of CD8αα+ T cells producing IL-17A in the psoriatic lesions was 24.85% ± 4.25%, while CD8αα+ T cells in the control skin tissues did not produce IL-17A. There was a significant difference in the proportion of CD8αα+ T cells producing IL-17A between the psoriatic skin lesions and control skin tissues （t = 5.853, P < 0.01）. Conclusion CD8αα+ T cells infiltrating psoriatic lesions are effector memory T cells, and may contribute to the occurrence and development of psoriasis by producing IL-17A.

Key words: Psoriasis, CD8?positive T?lymphocytes, Immunophenotyping, Interleukin?17, Fluore?scent antibody technique

中图分类号: 

  • R751