中华皮肤科杂志 ›› 2017, Vol. 50 ›› Issue (5): 355-359.

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氟康唑诱导耐药的阿萨希毛孢子菌ERG11基因表达研究

丁潇1,夏志宽2,张德全3,杨蓉娅4   

  1. 1. 安徽医科大学陆军总医院临床学院；陆军总医院全军皮肤损伤修复研究所
    2. 陆军总医院全军皮肤损伤修复研究所
    3.
    4. 北京军区总医院皮肤科
  • 收稿日期:2016-08-05 修回日期:2016-11-15 发布日期:2017-04-28
  • 通讯作者: 杨蓉娅 E-mail:Yangrya@sina.com
  • 基金资助:
    ERG11基因及CDR1基因在阿萨希毛孢子菌唑类耐药中的作用

Expression of the ERG11 gene in fluconazole?resistant Trichosporon asahii

DING Xiao1,Zhi-Kuan XIA2, 1,   

  • Received:2016-08-05 Revised:2016-11-15 Published:2017-04-28

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摘要： 目的 探讨ERG11基因在阿萨希毛孢子菌耐药的作用以及基因表达量与药物浓度间的关系。方法 通过氟康唑体外诱导获得阿萨希毛孢子菌耐药菌株，将敏感株与耐药株同时置于含0 ~ 64 μg /ml浓度氟康唑的培养基中培养，用实时PCR检测ERG11基因的表达情况。结果 在无药情况下，阿萨希毛孢子菌耐药株组ERG11基因表达（7.542 ± 5.311）高于敏感株组（1.014 ± 0.012），两组比较t = 3.002，P = 0.03。在不同浓度氟康唑作用下，耐药株组ERG11 mRNA水平分别为9.183 ± 3.226，13.657 ± 5.428，15.292 ± 7.007，13.720 ± 8.550，13.949 ± 2.960，13.123 ± 6.429，亦高于敏感株组3.281 ± 2.068，3.459 ± 1.923，3.242 ± 2.530，3.651 ± 0.728，3.969 ± 1.924，3.824 ± 1.875，均P < 0.05。但ERG11表达量与氟康唑浓度之间差异无相关性（耐药株rs = 0.229，P = 0.096；敏感株rs = 0.166，P = 0.357）。结论 阿萨希毛孢子菌ERG11基因过表达与氟康唑耐药有关。ERG11基因mRNA表达与氟康唑浓度之间无相关性。

关键词: 基因, ERG11

Abstract: Ding Xiao, Xia Zhikuan, Zhang Dequan, Yang Rongya Department of Dermatology, Clinical College of General Hospital of Beijing Military Command, Anhui Medical University, Beijing 100700, China （Ding X, Yang RY）; Institute of Skin Damage and Repair, General Hospital of Beijing Military Command of PLA, Beijing 100700, China （Xia ZK, Zhang DQ） Corresponding author: Yang Rongya, Email: yang_rya@sina.com 【Abstract】 Objective To investigate the role of the ERG11 gene in the drug resistance of Trichosporon asahii （T.asahii）, and to explore the relationship between the gene and drug concentrations. Methods Stable fluconazole?resistant strains of T.asahii were induced in vitro following exposure to a series of concentrations of fluconazole. Fluconazole?sensitive and ?resistant strains of T.asahii were separately cultured in the medium containing fluconazole at concentrations of 0, 0.25, 0.5, 1, 2, 4, 8, 16, 32 and 64 μg/ml. Real?time quantitative PCR was performed to determine the mRNA of ERG11 gene. Results In fluconazole?free medium, the fluconazole?resistant strain of T.asahii showed significantly increased mRNA of the ERG11 gene compared with the fluconazole?sensitive strain （7.542 ± 5.311 vs. 1.014 ± 0.012, t = 3.002, P = 0.03）. Additionally, the mRNA of ERG11 gene was also significantly higher in the fluconazole?resistant strains than the fluconazole?sensitive strains in the culture medium containing fluconazole at different concentrations of 0.25 （9.183 ± 3.226 vs. 3.281 ± 2.068）, 0.5 （13.657 ± 5.428 vs. 3.459 ± 1.923）, 1 （15.292 ± 7.007 vs. 3.242 ± 2.530）, 2 （13.720 ± 8.550 vs. 3.651 ± 0.728）, 4 （13.949 ± 2.960 vs. 3.969 ± 1.924） and 8 （13.123 ± 6.429 vs. 3.824 ± 1.875） μg/ml （all P < 0.05）. However, no significant correlation was observed between the mRNA of ERG11 gene and fluconazole concentrations （fluconazole?resistant strains: rs = 0.229, P = 0.096; fluconazole?sensitive strains: rs = 0.166, P = 0.357）. Conclusion Over of ERG11 gene is associated with fluconazole resistance in T.asahii, but there is no correlation between the mRNA of ERG11 gene and fluconazole concentrations.

引用本文

丁潇 夏志宽 张德全 杨蓉娅. 氟康唑诱导耐药的阿萨希毛孢子菌ERG11基因表达研究[J]. 中华皮肤科杂志, 2017,50(5):355-359. doi:

DING Xiao Zhi-Kuan XIA. Expression of the ERG11 gene in fluconazole?resistant Trichosporon asahii[J]. Chinese Journal of Dermatology, 2017, 50(5): 355-359.doi: