中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (12): 733-735.

• 论著 • 上一篇    下一篇

DNaseⅠ消化前后的链球菌抗原对寻常性银屑病外周血单一核细胞增殖反应的影响

蔡怡华, 郑捷   

  1. 上海交通大学医学院附属瑞金医院皮肤科 200025
  • 收稿日期:2007-01-15 出版日期:2007-12-15 发布日期:2018-09-29
  • 基金资助:
    上海第二医科大学博士点基金(BXJ0317)

Effects of DNaseⅠ-treated streptococcal antigens on peripheral blood mononudear cell proliferation in patients with psoriasis vulgaris

CAI Yi-hua, ZHENG Jie   

  1. Department of Dermatology, Ruijin Hospital, Medical School, Shanghai-Jiao Tong University, Shanghai 200025, China
  • Received:2007-01-15 Online:2007-12-15 Published:2018-09-29

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摘要： 目的 观察DNA酶(DNaseⅠ)消化前后的链球菌抗原对寻常性银屑病患者外周血单一核细胞(PBMC)增殖反应的影响.方法 寻常性银屑病患者15例,正常人10例.取外周抗凝血,分离并培养PBMC,分别使用A型B溶血型链球菌全菌抗原(SA)和DNaseⅠ消化后的链球菌抗原(DNaseⅠ-SA)刺激PBMC,72h后采用3H-TdR掺入法检测并比较PBMC增殖反应的差异.结果 DNaseⅠ-SA与SA比较,无论在高浓度还是低浓度时,前者刺激患者PBMC增殖反应的能力均明显低于后者(P<0.01);在正常人,高浓度时DNaseⅠ-SA对PBMC刺激的增殖反应与SA比较差异无统计学意义(P>0.05),但在低浓度时DNaseⅠ-SA刺激后细胞增殖反应的能力低于SA(P<0.05).进一步比较患者和正常人对SA和DNaseⅠ-SA刺激后增殖反应的差异,发现当抗原浓度为25μg/mL时,患者PBMC对SA刺激后的增殖能力显著高于正常人(P<0.05);患者PBMC对DNaseⅠ-SA三种剂量刺激后的增殖反应与正常人比较差异无统计学意义(P>0.05);同时,患者对SA和DNaseⅠ-SA刺激后增殖反应的差值高于正常人,差异有统计学意义(P<0.05).结论 链球菌DNA可能参与了感染诱发银屑病的发生.

关键词: 银屑病, 链霉菌属, DNaseⅠ, 细胞增殖

Abstract: Objective To investigate the effects of DNaseⅠ-treated streptococcal antigens on peripheral blood mononuclear cells(PBMC)proliferation in psoriasis patients.Methods PBMC from 15 patients with psoriasis and 10 healthy controls were stimulated with three concentrations(2,10,25μg/mL) of streptococcal antigens(SA), nd DNaseⅠ-treated streptococcal antigens(DNaseⅠ-SA).After 72 h of culture,cellular proliferation was determined by 3H thymidine incorporation.Results The DNaseⅠ-SA,at all the concentrations tested,was less potent than the corresponding concentrations of SA(P<0.01)in stimulating the proliferation of PBMC from psoriasis patients.In normal controls,no difference was observed between 25μg/mL of DNaseⅠ-SA and SA(P>0.05),however,the lower concentrations(2,10μg/mL) of DNaseⅠ-SA were less potent than those of SA(P<0.05).The proliferation level of PBMC in response to 25μg/mL of SA was significantly higher in patients than in normal controls(P<0.05),while there was no significant difference in response to DNaseⅠ-SA of all three concentrations between patients and normal controls(P>0.05).Also,the extent of proliferation response to SA and DNaseⅠ-SA was higher in the patients than in the controls(P<0.05).Conclusion Streptococcal DNA may participate in the development of psoriasis caused by infection.

Key words: Psoriasis, Streptomyces, DNaseⅠ, Cell proliferation