角蛋白置换肽配体对银屑病患者外周血T细胞的抑制作用

中华皮肤科杂志

角蛋白置换肽配体对银屑病患者外周血T细胞的抑制作用

沈柱¹, 陈凌², 王刚¹, 史晓蔚¹, 刘玉峰¹

1. 第四军医大学西京医院皮肤科, 西安, 710032;
2. 第三军医大学大坪医院野战外科研究所皮肤科

收稿日期:2006-12-01 出版日期:2007-10-15 发布日期:2007-10-15
通讯作者: 沈柱,email:shen_zhu@sohu.com E-mail:shen_zhu@sohu.com
基金资助:
国家自然科学基金(30600558)

In vitro inhibition of peripheral blood T lymphocytes isolated from patients with psoriasis by altered peptide ligands of keratin

SHEN Zhu¹, CHEN Ling², WANG Gang¹, Sill Xiao-wei¹, LIU Yu-feng¹

Department of Dermatology, West China Hospital, Fourth Military Medical University, Xi'an 710032, China

Received:2006-12-01 Online:2007-10-15 Published:2007-10-15

摘要/Abstract

摘要： 目的筛选下调银屑病患者T细胞功能的置换肽配体。方法分离和纯化寻常型银屑病患者和健康人外周血T细胞,通过MTT法和ELISA法分别检测T细胞增殖情况和IFN-γ、IL-2、IL-4和IL-10等细胞因子的变化,筛选得到抑制银屑病患者外周血T细胞功能的APL。结果 APL 119R和355L可显著下调银屑病患者T细胞的增殖,分别与谷胱甘肽巯基转移酶和空白对照比较,在10μg/mL和100μg/mL浓度下,P<0.05;而对健康人T细胞无明显作用。同时二者可显著下调银屑病患者外周血T细胞的IFN-γ和IL-2,上调IL-4和IL-10的分泌表达,分别与相对应的野生型表位比较,在10μg/mL和100μg/mL浓度下,P<0.05。结论 APL 119R和355L具有体外下调银屑病患者外周血T细胞增殖和增强Th2极化的作用。

关键词: 银屑病, 角蛋白, 表位, T淋巴细胞, 置换肽配体

Abstract: Objective To evaluate the ability of altered peptide ligands(APLs)to inhibit the activities of psoriatic T cells.Methods Peripheral blood T cells were isolated from 52 patients with psoriasis and 48 healthy human controls.The APLs targeting T cell epitopes of keratin 17 were synthesized.The peripheral blood monocytes were incubated with the wild T cell epitopes,APLs or control peptides(glutathione Stransferase,GST).These monocytes were then used as antigen presenting cells to interact with T lymphocytes from the same individuals.MTT method was used to detect the proliferation of T lymphocytes,and ELISA assay to measure the levels of IFN-γ,IL-2,IL-4 and IL-10 in the culture supernatants of these T lymphocytes.Results Compared with GST and blank control,the APLs,119R and 355L at a concentration of 10 and 100μg/mL significantly down-regulated the proliferation of psoriatic T cells(P<0.05);no obvious inhibition of proliferation of cells from healthy controls was noted.APLs at 10 and 100μg/mL also decreased the secretion of IFN-γand IL-2 in the supernatants ofT cells,and increased the levels of IL-4 and IL-10 in comparison with the wild epitopes(all P<0.05).Conclusion Our findings show that APLs,119R and 355L,are capable of inhibiting the proliferation of psoriatic T cells and facilitating the differentiation to Th2 cells in vitro.

Key words: Psoriasis, Keratin, Epitopes, T-lymphocyte, Altered peptide ligand

沈柱, 陈凌, 王刚, 史晓蔚, 刘玉峰. 角蛋白置换肽配体对银屑病患者外周血T细胞的抑制作用[J]. 中华皮肤科杂志, 2007, 40(10): 625-627.

SHEN Zhu, CHEN Ling, WANG Gang, Sill Xiao-wei, LIU Yu-feng. In vitro inhibition of peripheral blood T lymphocytes isolated from patients with psoriasis by altered peptide ligands of keratin[J]. Chinese Journal of Dermatology, 2007, 40(10): 625-627.

参考文献

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