中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (3): 197-200.

• 论著 • 上一篇    下一篇

桥粒芯糖蛋白1、3在HaCaT细胞、A431细胞及原代角质形成细胞中的表达

李惠1,冯素英2,林麟2,周武庆3,3,徐浩翔4,李志量5,靳培英6   

  1. 1. 北京大学深圳医院
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
    3. 中国医学科学院北京协和医学院皮肤病研究所
    4. 中国医学科学院皮肤病医院（研究所）
    5. 中国医学科学院皮肤病研究所
    6. 中国医学科学院北京协和医学院皮肤病医院
  • 收稿日期:2013-05-13 修回日期:2013-12-20 发布日期:2014-03-01
  • 通讯作者: 冯素英 E-mail:fengsuying2015@163.com
  • 基金资助:
    国家自然科学基金

Expressions of desmoglein 1 and desmoglein 3 in primary keratinocytes and two keratinocyte cell lines HaCaT and A431

Li1, 3,3,Hao-Xiang XU3,li zhiliang3, 3   

  • Received:2013-05-13 Revised:2013-12-20 Published:2014-03-01

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摘要： 【摘要】 目的 测定不同类型角质形成细胞（KC）桥粒芯糖蛋白（DSG）1、DSG3及DSG1 mRNA、DSG3 mRNA表达。 方法 不同KC（HaCaT细胞、A431细胞及原代KC）培养后，直接免疫荧光观察细胞DSG1、DSG3的表达，定量聚合酶链反应（qPCR）测定DSG1 mRNA、DSG3 mRNA相对表达水平。 结果 DSG1与DSG3表达于三种角质形成细胞，荧光强度显示，A431细胞高于HaCaT细胞，HaCaT细胞高于原代KC。三种细胞均表达DSG1、DSG3 mRNA，原代KC DSG1与DSG3 mRNA的相对表达量分别为HaCaT细胞的291.7%及237.4%，差异有统计学意义（P < 0.01）；A431细胞DSG1 mRNA为HaCaT细胞的0.1%、DSG3 mRNA则为HaCaT细胞的18.8%，差异有统计学意义（P < 0.05）。 结论 三种KC均可用于对DSG1、DSG3的相关研究；相对而言，正常培养的A431细胞表面DSG1、DSG3表达较HaCaT细胞及原代KC高；原代KC DSG1、DSG3 mRNA水平较HaCaT细胞及A431细胞高。

关键词: 角蛋白细胞, 桥粒芯糖蛋白质1, 桥粒芯糖蛋白质3

Abstract: Li Hui, Feng Suying, Lin Lin, Zhou Wuqing, Xu Haoxiang, Li Zhiliang, Jin Peiying. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China Corresponding authors: Feng Suying, Email: fengsuying@medmail.com.cn; Lin Lin, Email: dllon3@163.com 【Abstract】 Objective To detect the mRNA and protein expressions of desmoglein 1 （DSG1） and DSG3 in different types of keratinocytes（KCs）. Methods Two keratinocyte cell lines HaCaT and A431, as well as primary keratinocytes from human abdomenal skin served as the object of this study. Direct immunofluorescence assay was performed to observe and quantify the expressions of DSG1 and DSG3, and quantitative PCR（qPCR） to determine the mRNA expressions of DSG1 and DSG3, in these cells. Results Both DSG1 and DSG3 were expressed in all the three types of keratinocytes, and the fluorescence intensity of DSG1 and DSG3 in HaCaT cells was higher than that in primary keratinocytes but lower than that in A431 cells. Similarly, all the keratinocytes expressed DSG1 and DSG3 mRNA, with the relative expression levels of DSG1 and DSG3 mRNA in primary keratinocytes being 291.7% and 237.4% of those in HaCaT cells respectively （both P < 0.01）, and those in A431 cells being 0.1% and 18.8% of those in HaCaT cells respectively （both P < 0.05）. Conclusions HaCaT cells, A431 cells and primary keratinocytes all can be used for the study of DSG1 and DSG3, of which, A431 cells show the strongest expressions of DSG1 and DSG3, and primary keratinocytes display the highest expressions of DSG1 and DSG3 mRNAs.

Key words: Keratinocytes, Desmoglein 1, Desmoglein 3

引用本文

李惠 冯素英 林麟 周武庆 徐浩翔 李志量 靳培英. 桥粒芯糖蛋白1、3在HaCaT细胞、A431细胞及原代角质形成细胞中的表达[J]. 中华皮肤科杂志, 2014,47(3):197-200. doi:

Li Hao-Xiang XU li zhiliang. Expressions of desmoglein 1 and desmoglein 3 in primary keratinocytes and two keratinocyte cell lines HaCaT and A431[J]. Chinese Journal of Dermatology, 2014, 47(3): 197-200.doi: