中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (3): 149-151.

• 论著 • 上一篇    下一篇

银屑病皮损中Toll样受体4、NF-κBp65、HSP60的表达

颜兰香1, 李浪2, 赵娟3, 谭琦4   

  1. 1. 中南大学湘雅二医院皮肤科, 长沙, 410011;
    2. 中南大学湘雅医院;
    3. 湖南省马王堆医院;
    4. 重庆医科大学附属儿童医院
  • 收稿日期:2006-03-06 出版日期:2007-03-15 发布日期:2007-03-15
  • 通讯作者: 李浪,email:lilang0101@yahoo.com.cn E-mail:lilang0101@yahoo.com.cn

The expression of Toll-like receptor 4, NF-κBp65 and HSP60 in psoriatic lesions

YAN Lan-xiang1, LI-Lang2, ZHAO Juan3, TAN Qi4   

  1. Department of Dermatology, Xiangya Second Hospital, Central South University, Changsha 410011, China
  • Received:2006-03-06 Online:2007-03-15 Published:2007-03-15

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摘要： 目的 研究Toll样受体4(TLR4)、NF-κBp65、HSP60在银屑病患者皮损中的表达水平及其与银屑病严重程度的关系.方法 采用S-P免疫组化法检测30例进行期寻常性银屑病皮损及10例正常皮肤石蜡标本中的TLR4、NF-κBp65、HSP60的表达,对其在皮损中的表达水平进行相关性分析,并将结果与PASI评分进行相关性分析.结果 与正常人对照组相比,寻常性银屑病皮损角质形成细胞中的TLR4、NF-κBp65、HSP60的表达明显上调,差异有统计学意义(P<0.05).TLR4、NF-κBp65与HSP60表达水平与患者PASI评分之间均存在正相关(P<0.01).银屑病患者TLR4、NF-κBp65、HSP60的表达水平间均呈正相关(P<0.05).结论 TLR4、NF-κBp65、HSP60的表达上调可能与银屑病发病及严重程度有关.HSP60可能与TLR4/NF-κBp65信号转导途径有关.

关键词: 沙眼衣原体, 抗菌药, 药敏实验, 银屑病, NF-κB, 监控蛋白60, Toll样受体4

Abstract: Objective To investigate the expressions of Toll-like receptor 4 (TLR4),nuclear factor-KB (NF-κB) p65 and heat shock-protein 60(HSP60)in progressive psoriasis wlgaris and their correlation with disease severity in psoriatic patients.Methods Paraffin-embedded tissue samples were prepared from the lesions of patients(n=30)with progressive psoriasis vulgaris and from normal control skin(n=10), then were analyzed by SP immunohistochemical technique for the expression of TLR4, NF-κBp65 and HSP60.Correlation analysis was performed among the expression levels of three factors as well as between the levels and disease severity.Results The expressions of TLR4, NF-KBp(5 and HSP60 were significantly higher in the lesions than those in the controls(t=7.33, 8.14, 3.53, respectively, all P<0.05).The expressions of TLR4, NF-KBp65 and HSP60 positively correlated individually with the psoriasis area and severity index (PASI)scores in patients(r=0.908, 0.732, 0.927, respectively, all P<0.01).Positive correlation was also found between the expression of any two factors(TLR4; NF-KBp65 or HSP60)in the lesions(all P<0.05)Conclusions The up-regulation of TLR4, NF-κBp65 and HSP60 expressions may be related to the pathogenesis and severity of psoriasis.HSP60 may be involved in the TLR4/NF-κB signal pathway.

Key words: Psoriasis, NF-kappa B, Chaperonin 60, Toll-like receptor 4