中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (3): 146-148.

• 论著 • 上一篇    下一篇

斑块状银屑病表皮干扰素-γ受体mRNA表达的研究

陈敏, 崔盘根, 曹元华, 弓娟琴, 解士海, 陈志强   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京, 210042
  • 收稿日期:2006-04-04 出版日期:2007-03-15 发布日期:2007-03-15
  • 通讯作者: 陈志强,email:zqchen@hotmail.com E-mail:zqchen@hotmail.com
  • 基金资助:
    江苏省人事厅“六大人才高峰”项目资助;江苏省自然科学基金部分资助(BK2001195);国家自然科学基金部分资助(30170863)

The mRNA expression of IFN-gamma receptor in epidermis of patients with plaque psoriasis

CHEN Min, CUI Pan-gen, CAO Yuan-hua, GONG Juan-qin, XIE Shi-hai, CHEN Zhi-qiang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2006-04-04 Online:2007-03-15 Published:2007-03-15

PDF (PC)

28

赞

摘要： 目的 探讨斑块状银屑病患者表皮干扰素-γ受体mRNA的表达及其在发病机制中的作用.方法 采集28例斑块状银屑病患者皮损及周围外观正常皮肤,28例正常人皮肤作为对照,分离表皮,逆转录聚合酶链反应(RT-PCR)法检测干扰素-γ受体mRNA表达水平.PASI评分法评估银屑病的严重程度.结果 斑块状银屑病患者皮损和非皮损表皮干扰素-γ受体mRNA的表达率为100%,对照样本的表达率为17.86%(5/28);患者皮损、非皮损和对照表皮干扰素-γ受体mRNA的表达水平均值分别为1.002±0.563、0.188±0.095、0.005±0.012,皮损处的表达水平明显高于非皮损处(t=7.54,P<0.01),非皮损处明显高于正常人对照;进行期与稳定期皮损的表达水平分别为1.210±0.489和0.379±0.163,进行期显著高于稳定期(t=4.37,P<0.01);而皮损处的表达水平与PASI评分值无相关性.结论 表皮干扰素-γ受体mRNA的表达可能与银屑病皮损的形成和病情的活动性有关.

关键词: 过敏原, 点刺试验, 阿罗格, 过敏性疾病, 银屑病, 受体,干扰素

Abstract: Objective To explore the mRNA expression of IFN-gamma receptor in epidermis of patients with plaque psoriasis and its role in the pathogenesis of psoriasis.Methods Lesional and non-lcsional specimens were obtained from psoriatic patients(n=28)and normal controls(n=28).Epidermis was detached, and subjected to reverse transcriptase polymerase chain reaction(RT-PCR)for the measurement of IFN-gamma receptor mRNA.The disease severity was evaluated by psoriasis area and severity index(PASI).Results The IFN-gamma receptor mRNA was expressed in all lesional and non-lesional epidermis from the patients, while in only 17.86%(5/28)from controls.The mean value of IFN-gamma receptor mRNA in the lesional epidermis was significantly higher than that in the non-lcsional epidermis(1.002±0.563 vs 0.188±0.095, t=7.54, P<0.01),and the latter was significantly elevated compared with the controls(0.188±0.095 vs 0.005±0.012, t=10.17, P<0.01).The expression level of IFN-gamma receptor mRNA was significantly higher in active lesions than in stable lesions(1.210±0.489 vs 0.379±0.163, t=4.37, P<0.01).However, no correlation was found between the expression levels of IFN-gamma receptor mRNA and disease severity.Conclusions The mRNA expression of IFN-gamma receptor may be related to the lesion formation and disease severity in psoriatic patients.

Key words: Psoriasis, Receptor, interferon