中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (4): 207-209.

• 论著 • 上一篇    下一篇

蕈样肉芽肿皮损中增生性T细胞与树突细胞的研究

冯爱平, 赵静, 吴艳, 钱悦, 陈思远, 连昕, 涂亚庭   

  1. 华中科技大学同济医学院附属协和医院皮肤科 武汉 430022
  • 收稿日期:2005-04-14 发布日期:2006-04-15

Proliferating T cells and dendritic cells in the skin lesions of patients with mycosis fungoides

FENG Ai-ping, ZHAO Jing, WU Yan, QIAN Yue, CHEN Si-yuan, LIAN Xin, TU Ya-ting   

  1. Department of Dermatology, Xiehe Hospital, Tongji Medical College, Huazhong University for Science and Technology, Wuhan 430022, China
  • Received:2005-04-14 Published:2006-04-15

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摘要： 目的 探讨蕈样肉芽肿(MF)不同时期浸润性皮损中增生性T细胞与树突细胞的分布特征。方法 皮肤肿瘤组织石蜡切片,单克隆抗体免疫组化染色。结果 MF皮损中Ki-67+细胞和皮肤淋巴细胞相关抗原阳性(CLA+)细胞均明显增多,多数Ki-67+细胞CD4+和cLA+,MF肿瘤期真皮浸润组织中的Ki-67+细胞明显多于斑片期/斑块期(P<0.01),且形态多不规则,异型性明显;未见CD83+/Ki-67+树突细胞,但树突细胞周围可见大量的Ki-67+增生性T细胞,CD83+树突细胞与Ki-67+肿瘤T细胞紧密接触。结论 CLA+/Ki-67+T细胞在MF皮损中表达的程度和免疫病理特征对于MF的早期发现和转化进展可能具有免疫病理学诊断意义。

关键词: 淋巴瘤,T细胞,皮肤, 树突细胞, Ki-67抗原, T淋巴细胞, CLA

Abstract: Objective To evaluate the profile of proliferating T cells and dendritic cells (DCs) in the skin infiltrates of patients with mycosis fungoides (MF) in different stages. Method Paraffin section and immunohistochemisty with monoclonal antibody were used to detect the expression of special antigen per section. Results The numbers of Ki-67+ cells and cutaneous lymphocyte-associated antigen (CLA+) cells both increased significantly in the skin infiltrates of MF. Most Ki-67+ cells expressed both CLA and CD4 antigen. The number of Ki-67+ cells was significantly higher (P<0.01) in the skin infiltrates in tumor stage than that in plaque stage, and most of the cells were irregular in shape with obvious heteromorphism. No CD83+/Ki-67+ DC was observed. However, the DCs were always surrounded by Ki-67+ cells, and CD83+ DCs were closely contacted with Ki-67+ cells. Conclusion The number and immunopathologic feature of CLA+/Ki-67+ cells in the skin infiltrates of MF may have important diagnostic value for the early diagnosis and evaluation of prognosis of MF.

Key words: Lymphoma, T-cell, cutaneous, Dendritic cells, K. i-67 antigen, T-lymphocytes, CLA