中华皮肤科杂志 ›› 2015, Vol. 48 ›› Issue (1): 33-36.

• 论著 • 上一篇    下一篇

卡介菌多糖核酸对Nc/Nga小鼠特应性皮炎的保护作用

郭亚南1,王丽丽2,文海泉3,任勇1,李颂4   

  1. 1. 山东大学附属省立医院
    2. 济南市第五人民医院
    3. 长沙中南大学湘雅二医院皮肤科
    4. 山东大学附属省立医院皮肤科
  • 收稿日期:2014-06-24 修回日期:2014-08-15 发布日期:2019-06-12
  • 通讯作者: 李颂 E-mail:lisongpcx@163.com

Protective effect of polysaccharide nucleic acid fraction of bacillus Calmette-Guerin against atopic dermatitis in Nc/Nga mice

  • Received:2014-06-24 Revised:2014-08-15 Published:2019-06-12

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摘要： 目的 探讨卡介菌多糖核酸（BCG-PSN）对Nc/Nga小鼠特应性皮炎皮损的预防作用及可能机制。 方法 外用二硝基氯苯（DNCB）反复刺激新生Nc/Nga小鼠，通过皮炎评分、搔抓行为、组织病理学、免疫学指标评价皮炎损伤程度，观察BCG-PSN对小鼠皮损的预防作用。 结果 出生后4周内重复注射BCG-PSN，可以显著改善DNCB致敏Nc/Nga小鼠皮损炎症程度，降低皮炎评分以及小鼠的搔抓行为，不同浓度BCG-PSN组之间搔抓频率差异无统计学意义。BCG-PSN可以降低血浆中IgE的含量，呈剂量依赖性。0.5 mg/kg BCG-PSN可增加皮损中分泌干扰素（IFN）γ的细胞数量。不同剂量BCG-PSN均可降低脾单一核细胞培养上清液中IL-4的含量，增加IL-12的浓度。 结论 早期注射BCG-PSN可通过促进分泌IFN-γ的细胞增生，增加IL-12合成，降低IL-4和IgE的含量，从而预防Nc/Nga小鼠皮炎发生。

关键词: 皮炎，特应性, 小鼠，Nc/Nga, 树突细胞, 卡介菌多糖核酸

Abstract: Guo Yanan*, Wang Lili, Wen Haiquan, Ren Yong, Li Song. *Shandong Provincial Hospital, Shandong University, Jinan 250021, China Corresponding author: Li Song, Email: 123250349@qq.com 【Abstract】 Objective To evaluate the protective effect of polysaccharide nucleic acid fraction of bacillus Calmette-Guerin （BCG-PSN） against atopic dermatitis （AD） in Nc/Nga mice, and to explore its possible mechanism. Methods Sixteen Nc/Nga mice were classified into normal control group （n = 4）, low-concentration BCG-PSN group （n=5） and high-concentration BCG-PSN group （n = 7） to be subcutaneously injected with sodium chloride physiological solution, BCG-PSN of 0.1 mg/kg and 0.5 mg/kg respectively, at 1, 8, 15 and 22 days of age. Dinitrochlorobenzene （DNCB） was repeatedly and topically applied to these Nc/Nga mice to induce AD-like lesions at 49 days of age. The preventive effect of BCG-PSN against AD was evaluated by dermatitis scores, scratching frequency, histopathological manifestations and immunological parameters （including IgE, interleukin （IL）-4 and -12, and interferon （IFN）-γ）. Results Repeated injection of BCG-PSN within 4 weeks after birth significantly decreased the severity of DNCB-induced AD-like lesions, dermatitis scores and scratching behavior in Nc/Nga mice. There was no statistical difference in scratching frequency between the high- and low-concentration BCG-PSN groups. BCG-PSN treatment reduced the plasma level of IgE in Nc/Nga mice in a dose-dependent manner. BCG-PSN at 0.5 mg/kg increased the number of cells secreting IFN-γ in skin lesions of mice. Both doses of BCG-PSN down-regulated IL-4 level, but up-regulated IL-12 level in the culture supernatant of spleen mononuclear cells from mice. Conclusion Early injection of BCG-PSN could protect Nc/Nga mice against dermatitis by promoting the proliferation of IFN-γ-secreting cells, increasing the synthesis of IL-12, and reducing the levels of IL-4 and IgE.

Key words: Dermatitis, atopic, Mice, Nc/Nga, Dendritic cells, BCG0-PSN

中图分类号: 

  • R751.05

引用本文

郭亚南 王丽丽 文海泉 任勇 李颂. 卡介菌多糖核酸对Nc/Nga小鼠特应性皮炎的保护作用[J]. 中华皮肤科杂志, 2015,48(1):33-36. doi: