中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (5): 316-319.

• 论著 • 上一篇    下一篇

淋球菌对头孢曲松的敏感性及淋球菌多抗原测序分型研究

陈绍椿1,尹跃平2,戴秀芹3,孙厚华4,于瑞星5,韩燕6,陈祥生7   

  1. 1. 中国医学科学院皮肤病研究所，中国疾病预防控制中心性病控制中心
    2. 南京 中国医学科学院北京协和医学院皮肤病研究所
    3. 中国医学科学院皮肤病研究所
    4. 南京医科院皮研所
    5. 卫生部中日友好医院
    6. 中国医学科学院北京协和医学院皮肤病研究所
    7. 医科院皮研所
  • 收稿日期:2013-04-25 修回日期:2013-09-09 发布日期:2014-05-01
  • 通讯作者: 尹跃平 E-mail:yinyp@ncstdlc.org
  • 基金资助:
    国家自然科学基金青年科学基金;北京协和医学院协和青年基金

Ceftriaxone susceptibility testing and multi?鄄antigen sequence typing of Neisseria gonorrhoeae strains isolated in 2007 and 2012 from Nanjing, China

  • Received:2013-04-25 Revised:2013-09-09 Published:2014-05-01
  • Contact: Yue-ping YIN E-mail:yinyp@ncstdlc.org
  • Supported by:
    ;PUMC Youth Fund

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摘要： 【摘要】 目的 探讨南京市淋球菌对头孢曲松的敏感性以及相应菌株的淋球菌多抗原测序分型（NG-MAST）基因型别。 方法 2007年和2012年在中国疾病预防控制中心性病控制中心临床防治基地分别收集了204株和81株淋球菌，经过分离纯化及鉴定后，用琼脂稀释法测定其对头孢曲松的最小抑菌浓度（MIC）；菌株培养后利用试剂盒提取DNA，并进行淋球菌多抗原测序分型（NG-MAST）。 结果 测试的285株淋球菌MIC ≥ 0.060 μg/ml的菌株比例为63.2%，MIC ≥ 0.125 μg/ml的比例为31.6%。2012年MIC ≥ 0.060 μg/ml和MIC ≥ 0.125 μg/ml的菌株比例分别为44.4%和11.1%，2007年MIC ≥ 0.060 μg/ml和MIC ≥ 0.125 μg/ml的菌株比例分别为70.6%和39.7%。NG-MAST分型研究显示，285株淋球菌共有166个型别，菌株多样性较高，其中73种为已知型别，93种为新型别。2007年测定的所有菌株中以ST568（n = 13），ST270（n = 9），ST421（n = 7），ST2288（n = 5），ST1731（n = 4），ST1766（n = 4），ST1866（n = 4），ST1870（n = 4）等为主。2012年测定的所有菌株中以ST2318（n = 5），ST1053（n = 4），ST5990（n = 4），ST8726（n = 4）为主。相同NG-MAST型别的菌株具有相同或相近的MIC值。 结论 2012年与2007年菌株的优势型别有较大变化，某些型别与头孢药敏值有较强对应关系。NG-MAST分型可能作为分子生物学标记用于淋球菌耐药监测。

关键词: 奈瑟球菌，淋病, 抗药性, 头孢曲松

Abstract: Chen Shaochun, Yin Yueping, Dai Xiuqin, Sun Houhua, Yu Ruixing, Han Yan, Chen Xiangsheng. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College; National Center for Sexually Transmitted Disease Control, China Center for Disease Control and Prevention, Nanjing 210042, China Corresponding author: Yin Yueping, Email: yinyp@ncstdlc.org 【Abstract】 Objective To test the ceftriaxone susceptibility of Neisseria gonorrhoeae （NG） isolates from Nanjing city, and to assess their genotypes by using the NG multi-antigen sequence typing （NG-MAST） method. Methods A total of 204 NG strains isolated in 2007 and 81 in 2012 from Nanjing city were included in this study. The minimum inhibitory concentration （MIC） of ceftriaxone was determined for these strains using an agar dilution method. DNA was extracted by the Qiagen commercial kit from these strains followed by NG-MAST. Results All the isolates were susceptible to ceftriaxone （MIC, ≤ 0.25 μg/ml）. The MIC of ceftriaxone was ≥ 0.06 μg/ml for 63.2% of all the NG strains, 70.6% of those isolated in 2007 and 44.4% of those in 2012, and ≥ 0.125 μg/ml for 31.6 % of all the NG strains, 39.7% of those isolated in 2007, 11.1% of those in 2012. Totally, 166 genotypes were identified among the 285 isolates, of which, 73 had been reported, and 93 were previously unreported. The most prevalent genotype was ST568 （n = 13） in NG strains isolated in 2007, followed by ST270 （n = 9）, ST421 （n = 7）, ST2288 （n = 5）, ST1731 （n = 4）, ST1766 （n = 4）, ST1866 （n = 4）, ST1870 （n = 4）, while ST2318 （n = 5）, ST1053 （n = 4）, ST5990 （n = 4）, ST8726（n = 4） were the common genotypes in 2012. Those isolates with identical or similar genotypes tended to display similar MICs for ceftriaxone. Conclusions The prevalent genotypes of NG are markedly different between 2007 and 2012 in Nanjing region, and there is a strong association between the genotypes and ceftriaxone susceptibility of NG. NG-MAST results may serve as a genetic marker in the surveillance of antibiotic susceptibility in NG.

Key words: Neisseria gonorrhoeae, Drug resistance, Ceftriaxone

引用本文

陈绍椿 尹跃平 戴秀芹 孙厚华 于瑞星 韩燕 陈祥生. 淋球菌对头孢曲松的敏感性及淋球菌多抗原测序分型研究[J]. 中华皮肤科杂志, 2014,47(5):316-319. doi: