中华皮肤科杂志 ›› 2014, Vol. 47 ›› Issue (1): 15-18.

• 论著 • 上一篇    下一篇

特应性皮炎患者外周血和皮损miR-155的表达及其与Th17细胞相关性研究

马蕾1,薛海波2,管秀好3,舒春梅2,于娟4,张俊花5,运蓓蕾4,6   

  1. 1. 滨州医学院附属医院皮肤科
    2. 滨州医学院附属医院
    3. 沈阳 中国医科大学附属第一医院皮肤科
    4.
    5. 山东省滨州医学院附院皮肤科
    6. 沈阳市第七人民医院
  • 收稿日期:2013-05-27 修回日期:2013-07-09 发布日期:2014-01-01
  • 通讯作者: 薛海波 E-mail:xuehaibo@sina.com
  • 基金资助:
    山东省2010年科学技术发展计划;山东省自然科学基金;山东省医药卫生科技发展计划项目;山东省高等学校科技计划;滨州市科技发展计划

Expression of miR-155 in peripheral blood and skin lesions from as well as its relationship with Th17 cells in patients with atopic dermatitis

  • Received:2013-05-27 Revised:2013-07-09 Published:2014-01-01
  • Supported by:
    ;Projects of Medical and Health Technology Development Program in Shandong Province

PDF

6

摘要： 【摘要】 目的 探讨特应性皮炎（AD）患者外周血与皮损组织中miR-155、Th17细胞、Th17细胞特异性转录因子RORγt及效应性细胞因子IL-17的表达及其相关性。 方法 用实时荧光定量RT-PCR检测37例AD患者外周血单一核细胞（PBMC）中miR-155、RORγt及IL-17的mRNA表达水平，流式细胞仪检测Th17细胞比例，酶联免疫吸附试验（ELISA）检测血浆中IL-17的含量；并以33例性别、年龄匹配的健康儿童做对照。检测5例重度AD患者皮损与皮损周围组织中miR-155、RORγt及IL-17 mRNA的表达水平，并以5例正常皮肤组织标本为对照。 结果 AD患者PBMC中miR-155、RORγt及IL-17的mRNA表达水平均明显高于健康对照（分别为5.78 ± 1.78比1.82 ± 0.46，6.08 ± 1.04比1.64 ± 0.52，7.09 ± 1.75比1.71 ± 0.46，均P <0.01）；AD患者PBMC中Th17细胞比例及IL-17血浆含量均明显高于健康对照，分别为（1.78 ± 0.52）%比（0.47 ± 0.15）%，（32.51 ± 6.15） pg/ml比（11.80 ± 2.24） pg/ml，均P < 0.01。AD患者皮损、皮损周围组织及正常皮肤组织中miR-155、RORγt及IL-17 mRNA表达水平间比较，差异均具有统计学意义；皮损 > 皮损周围组织 > 正常皮肤组织，F值分别为41.803、17.040和37.064，均P < 0.01。AD患者PBMC中miR-155 mRNA表达水平与疾病严重程度评分、Th17细胞比例、RORγt mRNA表达水平、IL-17 mRNA表达水平及IL-17血浆含量呈正相关，r值分别为0.405、0.426、0.402、0.410、0.408，均P < 0.05；皮损及皮损周围组织中miR-155 mRNA表达水平与RORγt、IL-17的mRNA表达水平呈正相关，r值分别为0.428和0.435，均P < 0.05。 结论 AD患者miR-155、Th17细胞及其效应性细胞因子高表达可能与AD发病有关。

关键词: 皮炎，特应性, miR-155, Th17细胞

Abstract: Ma Lei *, Xue Haibo, Guan Xiuhao, Shu Chunmei, Yu Juan, Zhang Junhua, Yun Beilei. *Department of Dermatology, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong, China Corresponding author: Xue Haibo, Email: xuehaibo@sina.com 【Abstract】 Objective To detect the expressions of miR-155, T helper type 17 （Th17） cells, and Th17 cell-specific transcription factor RORγt and effector cytokine interleukin （IL）-17 in peripheral blood and skin lesions from, and to evaluate their relationship in, patients with atopic dermatitis （AD）. Methods Peripheral blood was obtained from 37 patients with AD and 33 age- and sex-matched healthy controls, and biopsy specimens from the lesional and perilesional skin of five patients with severe AD as well as from the normal skin of five healthy human controls. Real-time fluorescence-based reverse transcription （RT）-PCR was employed to measure the mRNA expression levels of miR-155, RORγt and IL-17 in peripheral blood mononuclear cells （PBMCs） and skin specimens, flow cytometry to detect the percentage of Th17 cells in PBMCs, enzyme-linked immunosorbent assay （ELISA） to determine the plasma concentration of IL-17. Statistical analysis was done using independent sample′s t test, one-way analysis of variance followed by the least significant difference test, and linear correlation analysis. Results Compared with the healthy controls, the patients with AD showed a significant increase in Th17 cell percentage（1.78% ± 0.52% vs. 0.47% ± 0.15%, P < 0.01）, mRNA expression levels of miR-155 （5.78 ± 1.78 vs. 1.82 ± 0.46, P < 0.01）, RORγt （6.08 ± 1.04 vs. 1.64 ± 0.52, P < 0.01） and IL-17 （7.09 ± 1.75 vs. 1.71 ± 0.46, P < 0.01）, as well as in the plasma concentration of IL-17 （（2.51 ± 6.15） pg/ml vs. （11.80 ± 2.24） pg/ml, P < 0.01）. There was a sequential decrease in the expression levels of miR-155, RORγt and IL-17 mRNA from lesional skin, perilesional skin to normal skin（F = 41.803, 17.040 and 37.064 respectively, all P < 0.01）. The miR-155 mRNA expression level in PBMCs was positively correlated with the SCORing Atopic Dermatitis （SCORAD） index, Th17 cell percentage, RORγt and IL-17 mRNA expression levels as well as IL-17 plasma concentration （r = 0.405, 0.426, 0.402, 0.410 and 0.408 respectively, all P < 0.05）. Similarly, the miR-155 expression level was positively correlated with RORγt and IL-17 mRNA expression levels in lesional and paralesional specimens （r = 0.428 and 0.435 respectively, both P < 0.05）. Conclusion The up-regulated expression of miR-155, Th17 cells and their effector cytokine IL-17 may be associated with the development of AD.

Key words: Dermatitis, atopic, miR-155, Th17 cells

引用本文

马蕾 薛海波 管秀好 舒春梅 于娟 张俊花 运蓓蕾. 特应性皮炎患者外周血和皮损miR-155的表达及其与Th17细胞相关性研究[J]. 中华皮肤科杂志, 2014,47(1):15-18. doi: