中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (9): 648-651.

• 论著 • 上一篇    下一篇

点阵Er：YAG激光对光老化豚鼠皮肤胶原纤维和弹性纤维的影响

邬宗周1,袁定芬2,章伟1,陈佳3,4,白梅2,邓辉2   

  1. 1. 上海市皮肤病医院
    2. 上海交通大学附属第六人民医院
    3. 南京 中国医学科学院北京协和医学院皮肤病研究所
    4. 同济大学附属上海市皮肤病医院
  • 收稿日期:2012-09-24 修回日期:2013-03-27 出版日期:2013-09-15 发布日期:2013-09-01
  • 通讯作者: 袁定芬 E-mail:yuandingfen@medmail.com.cn

Effects of fractional Er:YAG laser irradiation on collagen and elastic fibers in photoaged skin of Guinea pigs

  • Received:2012-09-24 Revised:2013-03-27 Online:2013-09-15 Published:2013-09-01

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摘要： 【摘要】 目的 探讨点阵Er：YAG激光照射对紫外线所致豚鼠光老化皮肤胶原纤维和弹性纤维的影响。方法 随机数字表法将60只豚鼠分为无处理组10只和模型组50只，中波紫外线（UVB）和长波紫外线（UVA）照射模型组豚鼠皮肤构建光老化模型，随机选择10只作为光老化模型组，余40只豚鼠分为2周、4周、8周、12周治疗组。点阵Er:YAG激光连续多脉冲照射治疗组豚鼠左侧背部（治疗侧）皮肤，HE染色、Masson染色、Weigert-van Gieson染色和碱水解-分光光度法比较照射后，治疗侧和对照侧皮肤组织学改变、胶原纤维、弹性纤维和羟脯氨酸含量。结果 UVA和UVB的最小红斑量4224 mJ/cm2和504 mJ/cm2隔日照射豚鼠背部皮肤，累积照射UVA 459.36 J/cm2和UVB 54.81 J/cm2，成功获得光老化模型。HE染色显示，治疗组治疗侧皮肤真皮浅层激光刺激区新生的胶原纤维和弹性纤维增加、排列致密。12周组Masson染色胶原面积比值治疗侧（0.70 ± 0.12）较对照侧（0.63 ± 0.08）均有增加，差异有统计学意义（t = 1.18，P < 0.05）；羟脯氨酸含量治疗侧［（4.73 ± 0.39） mg/g］较对照侧［（3.66 ± 0.85） mg/g］亦有增加，差异有统计学意义（t = 3.40，P < 0.05）。结论 点阵Er:YAG激光连续多脉冲照射可以刺激光老化豚鼠皮肤真皮浅层胶原纤维含量增加，纤细的弹性纤维新生。 【关键词】 皮肤衰老； 激光疗法，小剂量； 纤维胶原类； 弹性组织； 辐射损伤，实验性

关键词: 皮肤衰老, 激光疗法, 小剂量, 纤维胶原类, 弹性组织, 辐射损伤, 实验性

Abstract: WU Zong-zhou*, YUAN Ding-fen, ZHANG Wei, CHEN Jia, BAI Mei, DENG Hui. *Department of Dermatology, Shanghai Skin Disease Hospital, Shanghai 200050, China Corresponding author: YUAN Ding-fen, Email: yuandingfen@medmail.com.cn 【Abstract】 Objective To evaluate the effect of fractional Er:YAG laser therapy on collagen and elastic fibers in photoaged skin of ultraviolet-irradiated Guinea pigs. Methods Sixty Guinea pigs were randomly divided into the non-intervention group （n = 10） and model group （n = 50） by a random number table. To establish a model of photoaging, the 50 Guinea pigs were irradiated by ultraviolet A （UVA） and ultraviolet B （UVB） on the back every other day for two months. Subsequently, the 50 mice were equally classified into five groups: photoaging group receiving no laser therapy, as well as 2-week, 4-week, 8-week and 12-week group receiving one, one, two and three sessions of successive multi-pulsed fractional Er:YAG laser therapy, respectively, on the left side （treatment side） of the back, with the right side as the control side. The interval of laser therapy was four weeks. Skin biopsies were obtained from the right and left side of the back of Guinea pigs after the laser therapy, and subjected to haematoxylin and eosin （HE） staining, Masson staining and Weigert-van Gieson staining. The content of hydroxyproline was measured by using an alkaline-hydrolysis method. Results The minimal erythema dose for UVA and UVB was 4224 mJ/cm2 and 504 mJ/cm2 respectively on Guinea pig skin. The model for photoaging was successfully established in 50 Guinea pigs after two months of irradiation with the cumulative dose of UVA and UVB being 459.36 J/cm2 and 54.81 J/cm2 respectively. HE staining revealed an obvious increase in newly-growing collagen and elastic fibers, which were arranged densely, in the treatment side compared with the control side of the back of Guinea pigs after laser therapy. The area ratio of collagen fibers stained positive for Masson′s trichrome and content of hydroxyproline were significantly higher in the treatment side than in the control side of the 12-week group （0.70 ± 0.12 vs. 0.63 ± 0.08, t = 1.18, P < 0.05; （4.73 ± 0.39） mg/g vs. （3.66 ± 0.85） mg/g, t = 3.40, P < 0.05）. Conclusion Successive multi-pulsed fractional Er:YAG laser irradiation can result in an increase in collagen fibers and renewal of elastic fibers in superficial dermis of photoaged skin of Guinea pigs. 【Key words】 Skin aging; Laser therapy, low-level; Fibrillar collagens; Elastic tissue; Radiation injuries, experimental

Key words: Radiation injuries, experimental