中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (9): 644-647.

• 论著 • 上一篇    下一篇

CD99及miR-205在隆突性皮肤纤维肉瘤中的表达及意义

徐圣经1,饶彦婷1,赵志国2,尹雪锋1,张彩萍3,马朝东4,5,张宇5,陈建华1,丁克云5   

  1. 1. 镇江市第一人民医院
    2. 镇江市第一人民医院 （江苏大学附属人民医院）皮肤科
    3. 中国医学科学院、中国协和医科大学皮肤病研究所
    4.
    5. 江苏大学附属人民医院
  • 收稿日期:2012-10-17 修回日期:2012-11-16 出版日期:2013-09-15 发布日期:2013-09-01
  • 通讯作者: 丁克云 E-mail:dingkeyun123@163.com

Expressions of CD99 and miR-205 in dermatofibrosarcoma protuberans and their significance

  • Received:2012-10-17 Revised:2012-11-16 Online:2013-09-15 Published:2013-09-01

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摘要： 【摘要】 目的 探讨隆突性皮肤纤维肉瘤（DFSP）及皮肤纤维瘤（DF）中CD99及miRNA的表达及临床意义。方法 免疫组化染色及Western印迹检测DFSP和DF中CD99蛋白表达。实时PCR检测miR-637、miR-576-5p、miR-23a、miR-23b、miR-625及miR-205在DFSP和DF中的表达差异。结果 免疫组化染色结果显示，CD99蛋白在DFSP（73.33%）中阳性表达率明显高于DF（6.77%），差异有统计学意义（P < 0.05）。Western印迹检测结果显示，DFSP组织中CD99蛋白含量（1例，CD99/黏着斑蛋白 = 11.289）明显高于DF（2例，DF1: CD99/黏着斑蛋白 = 4.743，DF2: CD99/黏着斑蛋白 = 2.402）。实时 PCR检测结果显示，与DF相比，DFSP中miR-205表达明显下降（DF ∶ DFSP = 1 ∶ 0.160）；生物信息学分析表明，miR-205可以作用于CD99 mRNA的3′UTR区域。结论 CD99及miR-205均可作为鉴别诊断DFSP和DF的分子标记物。 【关键词】 皮肤纤维肉瘤； 纤维瘤； 抗原，CD99； miR-205

关键词: 皮肤纤维肉瘤, 纤维瘤, 抗原，CD99, miR-205

Abstract: XU Sheng-jing*, RAO Yan-ting, ZHAO Zhi-guo, YIN Xue-feng, ZHANG Cai-ping, MA Chao-dong, ZHANG Yu, CHEN Jian-hua, DING Ke-yun. *Department of Dermatology, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212002, Jiangsu, China Corresponding author: DING Ke-yun, Email: dingkeyun123@163.com 【Abstract】 Objective To detect the expressions of CD99 and miRNAs in dermatofibrosarcoma protuberan （DFSP） and dermatofibroma （DF）, and to investigate their clinical implications. Methods This study included 30 DFSP and 15 DF paraffin-embedded tissue specimens collected at the Department of Pathology, Affiliated People's Hospital of Jiangsu University, from January 2002 to December 2010, as well as two DFSP and three DF fresh-frozen tissue specimens. The expression of CD99 was detected by immunohistochemistry in all of these paraffin-embedded specimens, and by Western blot in one DFSP and two DF fresh-frozen tissue specimens. Real-time PCR was carried out to determine the expression levels of miR-637, miR-576-5p, miR-23a, miR-23b, miR-625 and miR-205 in two DFSP and three DF fresh-frozen tissue specimens. Immunohistochemical results were compared by chi-square test. Results Immunohistochemistry showed that the expression rate of CD99 protein was statistically higher in DFSP than in DF paraffin-embedded tissue specimens （73.33% vs. 6.67%, P < 0.05）. As Western blot displayed, the CD99/vinculin ratio was higher in the fresh-frozen DFSP specimen （11.289） than in the two DF specimens （4.743 and 2.402 respectively）. Real-time PCR revealed that the expression intensity of miR-205 was lower in DFSP than in DF fresh-frozen tissue specimens （0.160 vs. 1）. Bioinformatics analysis showed that miR-205 could target the CD99 mRNA 3′-untranslated region. Conclusion Both CD99 and miR-205 may serve as valuable markers for the differential diagnosis between DFSP and DF. 【Key words】 Dermatofibrosarcoma; Fibroma; Antigens, CD99; miR-205

Key words: miR-205

中图分类号: 

  • R246.7