C57BL/6小鼠不同毛发周期的皮肤组织微RNA表达的初步研究

摘要/Abstract

摘要： 【摘要】目的构建小鼠毛发周期的动物模型，筛选差异表达的微RNA。方法 30只雌性6 ～ 8周龄近交系C57BL/6小鼠，体重15 ~ 18 g。1%水合氯醛麻醉，松香/石蜡拔毛法拔除小鼠背部毛发。在拔毛后第0、8、20天，分别取3只小鼠，颈椎脱臼法处死，背部相同部位平行于脊椎取皮肤组织，按常规方法提取总RNA，进行微RNA微阵列芯片检测并行数据分析，发现其中有差异表达的微RNA。结果与休止期毛发皮肤组织相比，在生长期上调表达的有：miR-690、obselote-49、miR-1308，下调表达的有：miR-291a-5p、miR-212；在退行期上调表达的有：miR-690、obselote-49、MiR-31，下调表达的有：miR-127-3p、miR-212。结论初步筛选出小鼠毛发周期中皮肤组织存在的7个差异表达的微RNA，为今后其功能研究提供方向。【关键词】毛发；微RNAs

关键词: 毛发, 微RNAs

Abstract: LI Zhong-ming*, XU Wen-rong, WANG Lei, SHI He-jian, FAN Wei-xin. *Department of Dermatology, Affiliated Wuxi People′s Hospital, Nanjing Medical University, Wuxi 214023, Jiangsu, China Corresponding authors: FAN Wei-xin, Email: fanweixi@medmail.com.cn 【Abstract】 Objective To screen differentially expressed microRNAs at different stages of hair cycle in a murine model. Methods This study included 30 inbred female C57BL/6 mice （age, 6 － 8 weeks; body weight, 15 － 18 grams）. Hair growth cycle was induced in the back skin of C57BL/6 mice by application of wax/rosin followed by depilation under anesthesia with 1% chloral hydrate. Three mice were sacrificed by cervical dislocation on day 0, 8 and 20 after the induction, and skin tissue was achieved from the same depilated areas parallel to the spine. Total RNAs were extracted from the murine skin and subjected to microarray analysis of microRNA expression. Results Compared with telogen skin, the murine anagen skin showed a higher expression of miR-690, obselote-49 and miR-1308, but a lower expression of miR-291a-5p and miR-212. The expressions of miR-690, obselote-49 and miR-31 were significantly up-regulated, while those of miR-127-3p and miR-212 were down-regulated in the catagen skin in comparison with the telogen skin. Conclusion Seven microRNAs are identified in this study to be differentially expressed in murine skin between different stages of hair cycle, which may provide a direction for future research. 【Key words】 Hair; MicroRNAs

中图分类号:

R751

李中明许文嵘王磊施和建范卫新. C57BL/6小鼠不同毛发周期的皮肤组织微RNA表达的初步研究[J]. 中华皮肤科杂志, 2013, 46(8): 570-573.

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