中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (5): 349-352.

• 论著 • 上一篇    下一篇

凋亡调控分子BclGL表达与系统性红斑狼疮患者外周血单一核细胞凋亡的研究

李茗芳1,罗娜2,于大堂3,陈方如1,倪兵4,郝飞5   

  1. 1. 重庆市第三军医大学西南医院皮肤科
    2. 第三军医大学西南医院皮肤科
    3. 重庆市第三军医大学新桥医院骨科
    4. 重庆市第三军医大学免疫研究所
    5. 陆军军医大学第一附属医院皮肤科
  • 收稿日期:2012-11-14 修回日期:2013-02-05 出版日期:2013-05-15 发布日期:2013-05-01
  • 通讯作者: 郝飞 E-mail:haofei62@medmail.com.cn
  • 基金资助:
    凋亡调控分子BclGL诱导Treg细胞凋亡在SLE发病中的作用及其分子机制

Expression of an apoptosis?鄄regulating molecule BclGL and apoptosis in peripheral blood monoclear cells from patients with systemic lupus erythematosus

  • Received:2012-11-14 Revised:2013-02-05 Online:2013-05-15 Published:2013-05-01
  • Contact: Hao Fei E-mail:haofei62@medmail.com.cn

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摘要： 【摘要】 目的 探讨系统性红斑狼疮（SLE）患者外周血单一核细胞（PBMC）中凋亡调控分子BclGL的表达及其意义。 方法 流式细胞仪检测20例活动期SLE患者（A-SLE）、18例非活动期SLE患者（I-SLE）以及30例健康对照者PBMC的细胞绝对数；用膜联蛋白V-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）细胞凋亡双染试剂盒检测各组PBMC早期凋亡率；荧光定量PCR技术检测各组PBMC中BclGL mRNA的表达量；Western 印迹检测BclGL蛋白表达量；酶联免疫吸附试验（ELISA）检测各组血清中干扰素（IFN）-α水平；采用SPSS13.0统计软件进行组间非参数Mann-Whitney或Kruskal-Wallis检验，并使用Pearson相关分析法分析SLE患者PBMC中BclGL表达量与细胞凋亡率及临床指标间的相关性。 结果 A-SLE患者外周血中PBMC细胞绝对数［（0.16 ± 0.06） × 109/L］低于I-SLE［（0.27 ± 0.14） × 109/L］及健康对照［（0.34 ± 0.23） × 109/L］（均P < 0.01）；A-SLE患者PBMC凋亡率（22.6% ± 1.1%）较I-SLE（16.4% ± 0.9%）及健康对照（10.1% ± 0.4%）升高，I-SLE患者也高于健康对照（均P < 0.01）；A-SLE患者PBMC中BclGL表达量高于I-SLE及健康对照（均P < 0.01）；SLE患者外周血血清IFN-α含量［（32.5 ± 2.2） μg/L］较健康对照［（15.5 ± 1.3） μg/L］增高（均P < 0.01）；SLE患者PBMC中上调的BclGL表达与PBMC凋亡率（r = 0.486，P < 0.01）及SLE疾病活动指数（SLEDAI）、抗核抗体（ANA）滴度、IFN-α水平呈正相关（r = 0.496，0.516，0.535，均P < 0.01），与患者补体C3水平呈负相关（r = －0.515，P < 0.01）。结论 SLE患者PBMC中BclGL分子表达上调可能参与SLE患者PBMC的凋亡异常，从而在SLE发病中起作用。 【关键词】 红斑狼疮，系统性； 外周血单一核细胞； BclGL； 细胞凋亡

关键词: 红斑狼疮，系统性, 外周血单一核细胞, BclGL, 细胞凋亡

Abstract: LI Ming-fang *, LUO Na, YU Da-tang, CHEN Fang-ru, NI Bing, HAO Fei. *Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China Corresponding author: HAO Fei, Email: haofei62@medmail.com.cn 【Abstract】 Objective To detect the expression of BclGL in peripheral blood mononuclear cells （PBMCs）from patients with systemic lupus erythematosus （SLE） and to investigate its significance. Methods Peripheral blood was obtained from 20 patients with active SLE （A-SLE）, 18 patients with inactive SLE（I-SLE） and 30 healthy controls. Flow cytometry was performed to calculate the number of PBMCs， flow cytometry combined with annexin V- fluorescein isothiocyanate （FITC）/propidium iodide （PI） staining to determine the early apoptotic rates of PBMCs, fluorescence-based quantitative PCR and Western blot to measure the expression of BclGL mRNA and protein, respectively. The serum level of interferon （IFN）-α was determined by enzyme-linked immunosorbent assay （ELISA）. Data were analyzed by using the software SPSS13.0. Mann-Whitney U-test or Kruskal-Wallis test was used for group comparisons. Pearson correlation coefficient test was applied to evaluate the relationship of BclGL expression with cell apoptotic rate and some clinical parameters. Results The number of PBMCs was significantly lower in patients with A-SLE than in those with I-SLE and healthy controls （（0.16 ± 0.06） × 109/L vs. （0.27 ± 0.14） × 109/L and （0.34 ± 0.23） × 109/L, both P < 0.01）. Increased apoptotic rate of PBMCs was observed in patients with A-SLE compared with those with I-SLE and healthy controls （（22.6 ± 1.1）% vs. （16.4 ± 0.9）% and（10.1 ± 0.4）%, both P < 0.01）, and in patients with I-SLE compared with the healthy controls （P < 0.01）. The mRNA and protein expressions of BclGL in PBMCs were both significantly higher in patients with A-SLE than in those with I-SLE and healthy controls （all P < 0.01）. A significant increase was observed in serum IFN-α level in the patients with SLE compared with the healthy controls （（32.5 ± 2.2） μg/L vs. （15.5 ± 1.3） μg/L, P < 0.01）. The mRNA expression of BclGL in PBMCs from patients with SLE was positively correlated with the apoptotic rate in PBMCs （r = 0.486, P < 0.01）, SLE disease activity index score （r = 0.496, P < 0.01）, titers of antinuclear antibodies （r = 0.516, P < 0.01） and serum IFN-α level （r = 0.535, P < 0.01）, but was negatively correlated with complement C3 level （r = －0.515, P < 0.01）. Conclusions The increased expression of BclGL in PBMCs may contribute to the abnormal apoptosis in PBMCs, which in turn takes part in the pathogenesis of SLE. 【Key words】 Lupus erythematosus, systemic; Peripheral blood mononuclear cells; BclGL; Apoptosis

Key words: Peripheral blood monoclear cells, BclGL, Cell apoptosis

中图分类号: 

  • R593.24+1