中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (1): 24-28.

• 论著 • 上一篇    下一篇

青鹏软膏对小鼠特应性皮炎模型的影响及机制研究

李园园1,李邻峰2   

  1. 1. 北京大学第三医院皮肤科
    2. 首都医科大学附属北京友谊医院皮肤科
  • 收稿日期:2012-03-19 修回日期:2012-06-21 出版日期:2013-01-15 发布日期:2013-01-01
  • 通讯作者: 李邻峰 E-mail:zoonli@sina.com

Effect of Qingpeng ointment on experimental atopic dermatitis in mice and its possible mechanisms

1,   

  • Received:2012-03-19 Revised:2012-06-21 Online:2013-01-15 Published:2013-01-01

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摘要： 目的 探讨外用青鹏软膏对实验性特应性皮炎（AD）的抑制作用及可能机制。 方法 用2，4-二硝基氟苯在BALB/c小鼠背部皮肤建立实验性特应性皮炎模型。将小鼠分为6组：模型组（不治疗），治疗组（分别外用100%、75%及50%青鹏软膏），基质对照组（外用青鹏软膏基质）和空白对照组。连续用药14 d，分别在用药第8天及第15天测量小鼠背部皮损厚度及质量，并对皮损取材进行HE染色，计数炎症细胞，用ELISA方法测定血清及局部皮损组织匀浆中细胞因子的表达。结果 青鹏软膏各浓度组在用药第8天及第15天均明显减轻皮肤炎症和水肿，减少淋巴细胞浸润。100%青鹏软膏组和75%青鹏软膏组在用药第8天及第15天，血清及皮损匀浆IL-4和IL-5水平显著低于模型组和基质对照组，差异有统计学意义（P < 0.05）。100%青鹏软膏组在用药15 d，血清IL-2、干扰素（IFN）-γ、肿瘤坏死因子（TNF）-α水平升高，皮损匀浆IFN-γ及TNF-α水平升高，与模型组及基质对照组比较，差异有统计学意义（P < 0.05）。 结论 青鹏软膏能缓解小鼠AD模型炎症，可能是通过调节Th1和Th2 平衡而起作用。

关键词: 皮炎，特应性, 青鹏软膏, 二硝基氟苯, 细胞因子类

Abstract: LI Yuan-yuan，LI Lin-feng. Department of Dermatology, Peking University Third Hospital, Beijing 100191, China Corresponding author: LI Lin-feng, Email: zoonl@tom.com 【Abstract】 Objective To investigate the effect of a topical Tibetan medicine, Qingpeng ointment, on atopic dermatitis in mice and its possible mechanisms. Methods A model of atopic dermatitis was established in 180 7-week-old BALB/c mice by repeatedly applying 100 μl of 0.15% 2, 4-dinitrofluorobenzene （DNFB） to shaved ventral skin and 50 μl of 0.15% DNFB to shaved dorsal skin. After the establishment of model, the mice were divided into 5 groups, i.e., model group receiving no treatment，three Qingpeng groups treated with 100%, 75% and 50% Qingpeng ointment respectively, vehicle control group treated with the vehicle of Qingpeng ointment. Thirty-six mice receiving neither DNFB sensitization nor Qingpeng ointment or vehicle treatment served as the blank control group. The treatment lasted 14 days. On day 8 and 15, serum samples and dorsal skin tissue samples were obtained from these mice. Skin samples underwent the measurement of thickness and weightness, observation of histopathological changes by using hematoxylin and eosin （HE） staining, enumeration of inflammatory cells infiltrating the dermis. Enzyme linked immunosorbent assay （ELISA） was carried out to determine the levels of several cytokines, including interleukin （IL）-2, -4 and -5, interferon （IFN）-γ, tumor necrosis factor （TNF）-α, in the sera and homogenate of the skin samples. Results All the 3 concentrations of Qingpeng ointment significantly inhibited skin inflammation induced by DNFB on day 8 and 15, with a decrease in the degree of edema and number of lymphocytes infiltrating the dermis. Compared with the model mice and vehicle-treated mice, those treated with 100% and 75% Qingpeng ointment showed a significantly lower level of IL-4 and IL-5 in both sera and homogenates of skin lesions on day 8 and 15 after the start of treatment （all P < 0.05）. On day 15, a statistical increase was observed in IL-2, IFN-γ and TNF-α in sera as well as in IFN-γ and TNF-α in homogenates of skin lesions from mice treated with 100% Qingpeng ointment compared with the model mice and vehicle control mice（all P < 0.05）. Conclusion Qingpeng ointment can inhibit the inflammation in mouse model of atopic dermatitis, likely by modulating the balance between type 1 and type 2 T helper cells. 【Key words】 Dermatitis, atopic; Dinitrofluorobenzene; Cytokines; Qingpeng ointment

Key words: Cytokines

中图分类号: 

  • R751