中华皮肤科杂志 ›› 2013, Vol. 46 ›› Issue (1): 20-23.

• 论著 • 上一篇    下一篇

茶多酚对CD8+ T细胞杀伤白癜风患者黑素细胞的保护作用研究

欧阳杰1,吴纪龙1,周妙妮2,付丽芳2,许爱娥3   

  1. 1. 杭州市第三人民医院
    2. 杭州市第三人民医院皮肤科
    3. 安徽医科大学附属杭州市第三人民医院皮肤科
  • 收稿日期:2012-05-09 修回日期:2012-07-13 出版日期:2013-01-15 发布日期:2013-01-01
  • 通讯作者: 许爱娥 E-mail:xuaiehz@msn.com
  • 基金资助:
    茶多酚治疗白癜风的作用机制研究;国家自然科学基金;浙江省自然科学基金

Protective effects of tea polyphenols against the destruction of melanocytes by CD8+ T cells from vitiligo patients

  • Received:2012-05-09 Revised:2012-07-13 Online:2013-01-15 Published:2013-01-01

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摘要： 目的 探讨茶多酚对CD8+T细胞杀伤白癜风患者黑素细胞的保护作用。方法 取白癜风患者白斑边缘处皮片组织，培养CD8+T淋巴细胞，细胞纯度用流式细胞仪进行鉴定，通过和黑素细胞共培养验证其针对黑素细胞的杀伤作用。将不同浓度茶多酚加入CD8+T细胞和黑素细胞共培养的体系，用流式细胞仪检测黑素细胞的凋亡。 结果 取白癜风皮损边缘表皮组织中成功培养CD8+T淋巴细胞，纯度达90%以上，高表达活化抗原CD137和CD69。CD8+T细胞和黑素细胞共培养后，可明显诱导黑素细胞凋亡。200 μg/ml和400 μg/ml浓度茶多酚处理均可以降低黑素细胞的凋亡率。 结论 白癜风皮损边缘组织来源的CD8+T细胞对白癜风患者黑素细胞有明显杀伤作用。茶多酚对抗CD8+T细胞杀伤黑素细胞，从而对黑素细胞有一定的保护作用。

关键词: 白癜风, T淋巴细胞，细胞毒性, 黑素细胞, 茶多酚

Abstract: OUYANG Jie, WU Ji-long, ZHOU Miao-ni, FU Li-fang, XU Ai-e. Department of Dermatology, Third People′s Hospital of Hangzhou, Hangzhou 310009, China Corresponding author: XU Ai-e, Email: xuaiehz@msn.com 【Abstract】 Objective To evaluate the protective effects of tea polyphenols against the destruction of melanocytes by CD8+ T cells from vitiligo patients. Methods Skin tissue was resected from the margin of vitiligo lesions followed by the isolation and culture of CD8+ T lymphocytes, and from the normal skin of vitiligo patients followed by the isolation and culture of melanocytes. Flow cytometry was carried out to evaluate the purity of CD8+ T cells. The melanocytes were cocultured with the CD8+ T cells at different ratios followed by the evaluation of killing effect of CD8+ T cells. Various concentrations （200 and 400 μg/ml） of tea polyphenols were added into the co-culture system of CD8+ T cells and melanocytes at a ratio of 5 ∶ 1 followed by an additional culture of 48 hours. Then, flow cytometry was performed to detect the apoptosis in melanocytes in the coculture system. Results CD8+ T lymphocytes were successfully obtained from the marginal area of vitiligo lesions with a purity of more than 90%, which highly expressed the antigens CD137 and CD69. The coculture with CD8+ T cells markedly accelerated the apoptosis in melanocytes, while the accelerative effect was inhibited by tea polyphenols of 200 and 400 μg/ml. Conclusions The CD8+ T cells infiltrating the edge of vitiligo lesions display a potential destructive effect on autologous melanocytes from vitiligo patients, and tea polyphenols have a protective effect against the destruction of melanocytes by CD8+ T cells. 【Key words】 Vitiligo; T-lymphocytes, cytotoxic; Melanocytes; Tea polyphenols

Key words: Tea polyphenols