中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (9): 620-622.

• 论著 • 上一篇    下一篇

雌激素、肼苯哒嗪和紫外线对SLE患者甲基转移酶活性的影响

施伟民1,王上上2,肖琴3,伍洲炜4,徐金华5   

  1. 1. 上海交通大学附属上海第一人民医院
    2. 上海市复旦大学附属华山医院皮肤科
    3. 上海市浦东医院皮肤科
    4. 上海市第一人民医院
    5. 复旦大学附属华山医院
  • 收稿日期:2010-03-23 修回日期:2010-04-22 发布日期:2010-09-10
  • 通讯作者: 施伟民 E-mail:shiweimin1234@medmail.com.cn
  • 基金资助:

    国家自然科学基金;上海市科委基金;上海市科委基金

Effect of estrogen, hydralazine and ultraviolet ray on DNA methyltransferase-1 activity in patients with systemic lupus erythematosus

  • Received:2010-03-23 Revised:2010-04-22 Published:2010-09-10
  • Contact: SHI Wei-Min E-mail:shiweimin1234@medmail.com.cn

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摘要：

目的 探讨雌激素、肼苯哒嗪、紫外线（UVB）照射诱导SLE发病的作用机制。方法 分离、培养10例SLE患者和9例正常人外周血单核细胞（PBMC），分别进行雌激素、肼苯哒嗪和UVB照射等干预。采用甲基转移酶（DNMT）激活/抑制法检测PBMC的DNMT1活性。 结果 SLE患者DNMT1活性（0.36 ± 0.24）较正常人对照（0.46 ± 0.17）差异无统计学意义（P > 0.05），雌激素干预后SLE患者DNMT1活性显著低于正常人对照（0.32 ± 0.18比0.46 ± 0.17，t = 1.725，P < 0.05），肼苯达嗪干预后SLE患者DNMT1活性显著低于正常人对照（0.33 ± 0.13比0.46 ± 0.17，t = 1.739，P < 0.05），UVB干预后SLE患者DNMT1活性显著低于正常人对照（0.30 ± 0.14比0.46 ± 0.17，t = 1.739，P < 0.05）。另外，肼苯达嗪干预的正常人 DNMT1活性（0.38 ± 0.12）也显著低于正常人（0.46 ± 0.17）对照（P < 0.05）。结论 雌激素、肼苯哒嗪和UVB照射等因素具有抑制SLE患者DNMT1活性的作用，提示三个诱发因素可能通过改变DNMT活性来诱导SLE发病。

关键词: 红斑狼疮, 系统性, 雌激素, 肼苯哒嗪, 紫外线

Abstract:

Objective To explore the mechanism underlying the induction of systemic lupus erythematosus（SLE） by estrogen, hydralazine and ultraviolet irradiation. Methods Peripheral blood mononuclear cells （PBMCs） were harvested from 10 patients with SLE and 9 normal human controls, and cultured with or without the intervention with estrogen, hydralazine or ultraviolet irradiation. The DNA methyltransferase-1 （DNMT1） activity of PBMCs was quantified by using DNMT activity/inhibition assay kit. Results No statistical difference was observed in DNMT1 activity between patients with SLE and normal controls （0.36 ± 0.24 vs 0.46 ± 0.17, P > 0.05）. A significant decrease was noted in DNMT1 activity in PBMCs from patients with SLE after intervention with estrogen （0.32 ± 0.18 vs 0.46 ± 0.17, t = 1.725, P < 0.05）, hydralazine （0.33 ± 0.13 vs 0.46 ± 0.17, t = 1.739, P < 0.05） and ultraviolet irradiation （0.30 ± 0.14 vs 0.46 ± 0.17, t = 1.739, P < 0.05） compared with that from normal human controls. The treatment with hydralazine also induced an attenuation of DNMT1 activity in PBMCs from normal human controls （0.38 ± 0.12 vs 0.46 ± 0.17, P < 0.05）. Conclusion Estrogen, hydralazine and ultraviolet irradiation can inhibit the DNMT1 activity of SLE patients, indicating that they may induce the initiation of SLE by altering the activity of DNMT1.

Key words: Lupus erythematosus, systemic, estrogen, hydralazine, ultraviolet