中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (9): 617-619.

• 论著 • 上一篇    下一篇

过敏性紫癜患者外周血Th17/Treg细胞平衡性检测

马蕾1,李晓红2,张玉杰3,张俊花3   

  1. 1. 滨州医学院附属医院皮肤科
    2. 吉林大学第四医院
    3. 山东省滨州医学院附院皮肤科
  • 收稿日期:2010-01-08 修回日期:2010-01-20 发布日期:2010-09-10
  • 通讯作者: 马蕾 E-mail:iwf50@sina.com

Detection of Th17/Treg cell balance in peripheral blood of patients with Henoch-Schonlein purpura

  • Received:2010-01-08 Revised:2010-01-20 Published:2010-09-10
  • Contact: Lei Ma E-mail:iwf50@sina.com

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摘要：

目的 探讨Th17细胞/Treg细胞（CD4+CD25+ Foxp3+ T细胞）失衡在过敏性紫癜（HSP）发病机制中的作用。方法 采用流式细胞仪检测59例HSP患儿外周血Th17细胞和Treg细胞的比例、实时定量RT-PCR检测Th17细胞与Treg细胞转录因子RORγt、Foxp3 mRNA的表达。同时以38例性别、年龄匹配的健康儿童做对照。结果 HSP组外周血Th17细胞比例（CD4+IL-17+/CD4+ T细胞，1.87% ± 0.56%）及RORγt mRNA表达量（7.71 ± 1.95），均显著高于正常人对照组（CD4+IL-17+/CD4+ T细胞：0.39% ± 0.15%，P < 0.01; RORγt mRNA：1.49 ± 0.57，P < 0.01）；HSP组外周血Treg细胞比例（CD4+CD25+Foxp3+/CD4+ T细胞，1.63% ± 0.44%）及Foxp3 mRNA的表达量（0.34 ± 0.11），均明显低于正常人对照组（CD4+CD25+Foxp3+/CD4+ T细胞：5.04% ± 1.44%，P < 0.01；Foxp3 mRNA：1.71 ± 0.69，P < 0.01）。HSP各组间Th17细胞、Treg细胞比例及RORγt、Foxp3 mRNA的表达量差异无统计学意义（P > 0.05）。结论 过敏性紫癜患者外周血Th17、Treg细胞水平及其转录因子表达量发生变化，其比例的失平衡可能参与过敏性紫癜的发病。

关键词: 过敏性紫癜, Th17细胞, Treg细胞, 失平衡

Abstract:

Objective To assess the role of Th17/Treg cell imbalance in the pathogenesis of Henoch-Sch?觟nlein purpura （HSP）. Methods Peripheral blood was obtained from 59 children with HSP and 38 age- and sex-matched healthy subjects. Flow cytometric analysis （FCM） was performed to detect the percentage of CD4+IL17+ T （Th17） cells and CD4+CD25+ Foxp3+ T （Treg） cells, real-time RT-PCR to measure the mRNA expressions of RORγt and Foxp3, which are specific transcription factors of Th17 and Treg cells, respectively. Results Patients with HSP showed a significant increase in the percentage of Th17 cells and mRNA level of RORγt （1.87% ± 0.56% vs 0.39% ± 0.15%, 7.71 ± 1.95 vs 1.49 ± 0.57, both P < 0.01） and a statistical decrease in the percentage of Treg cells and mRNA level of Foxp3 （1.63% ± 0.44% vs 5.04% ± 1.44%, 0.34 ± 0.11 vs 1.71 ± 0.69, both P < 0.01） compared with the normal controls. No significant difference was observed in the percentage of Th17 or Treg cells or the mRNA levels of RORγt or Foxp3 among patients with different types of HSP. Conclusion In patients with HSP, there is a change in the pecentage of Th17/Treg cells and expression of their transcription factors, which may lead to immune imbalance and contribute to the development of HSP.

Key words: Henoch–Sch?nlein purpura, Thl7 cell, Treg cell, imbalance