中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (3): 181-183.

• 论著 • 上一篇    下一篇

UVA辐射对HaCaT细胞分泌和表达趋化因子CXCL11/I-TAC的影响

耿士玲1,单士军1,张同威1,吴剑1,王志华1,肖汀1,何春涤1,陈洪铎2   

  1. 1. 中国医科大学附属第一医院皮肤科
    2. 中国医科大学第一附属医院皮肤科
  • 收稿日期:2009-04-22 修回日期:2009-05-25 出版日期:2010-03-15 发布日期:2012-03-31
  • 通讯作者: 耿士玲 E-mail:ling81111@sina.com
  • 基金资助:
    教育部创新团队;辽宁省创新团队;国家自然科学基金;教育部高等学校博士学科点专项科研基金

Influences of ultraviolet A （UVA） on the secretion and expression of chemokine CXCL11/I-TAC by HaCaT cells

GENG Shi-lingSHAN Shi-jun2,ZHANG Tong-wei2,WU Jian2,WANG Zhi-hua2,XIAO Ting2,HE Chun-di3,CHEN Hong-Duo 2   

  • Received:2009-04-22 Revised:2009-05-25 Online:2010-03-15 Published:2012-03-31
  • Contact: GENG Shi-ling E-mail:ling81111@sina.com
  • Supported by:
    ;National Natural Sciences Foundation of China

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摘要： 目的 探讨UVA对IFN-γ和TNF-α诱导的HaCaT细胞分泌和表达CXCL11/I-TAC的影响。方法 用ELISA检测不同剂量UVA（2、4、8 J/cm2）照射后培养24 h的HaCaT细胞上清中CXCL11/I-TAC分泌水平。用实时荧光定量PCR检测CXCL11/I-TAC mRNA表达水平。结果 正常培养的HaCaT细胞仅分泌和表达微量的CXCL11/I-TAC蛋白或mRNA。当用10 μg/L的IFN-γ和TNF-α联合刺激后，HaCaT细胞分泌或表达的CXCL11/ I-TAC显著升高。UVA在2、4、8 J/cm2呈剂量依赖性抑制CXCL11/I-TAC的分泌或表达。结论 UVA照射抑制角质形成细胞分泌和表达CXCL11/I-TAC，从而在一定程度上降低了对Th1/Tc1细胞的趋化。

关键词: HaCaT细胞, 紫外线, 趋化因子，CXC

Abstract: Objective To investigate the influences of UVA on the secretion and expression of chemokine CXCL11/I-TAC by HaCaT cells induced by interferon γ （IFN-γ） and tumor necrosis factor α （TNF-α）. Methods HaCaT cells were cultured in the presence of IFN-γ and TNF-α and irradiated with UVA of 2, 4 and 8 J/cm2, respectively; those cells receiving neither treatment with IFN-γ or TNF-α nor UVA irradiation served as the negative control, and those receiving only cytokine treatment but no irradiation as the positive control. After another 24-hour culture, enzyme-linked immunosorbent assay （ELISA） was performed to detect the protein levels of CXCL11/I-TAC in the supernatant of HaCaT cells, real time PCR to measure the mRNA expression of CXCL11/I-TAC in these HaCaT cells. Results As far as the negative control HaCaT cells were concerned, there was a minor secretion of CXCL11/I-TAC protein and expression of CXCL11/I-TAC mRNA. After treatment with IFN-γ and TNF-α of 10 μg/L, the protein and mRNA expressions of CXCL11/I-TAC were synergistically upregulated, whereas the induced secretion and expression of CXCL11/I-TAC by HaCaT cells were dose-dependently inhibited by UVA irradiation. Conclusions UVA irradiation inhibits the secretion and expression of CXCL11/I-TAC by HaCaT cells, which in turn suppresses the chemotaxis of Th1/Tc1 cells in some degree.

Key words: Ultraviolet