中华皮肤科杂志 ›› 2010, Vol. 43 ›› Issue (3): 178-180.

• 论著 • 上一篇    下一篇

槲皮素对鼠黑素细胞株B10BR细胞的抗氧化保护效应及对其生物学活性的影响

孙学成1,关翠萍2,洪为松2,林福全2,许爱娥3   

  1. 1. 安徽医科大学杭州临床学院
    2. 杭州市第三人民医院
    3. 安徽医科大学附属杭州市第三人民医院皮肤科
  • 收稿日期:2009-04-22 修回日期:2009-05-19 出版日期:2010-03-15 发布日期:2012-03-31
  • 通讯作者: 孙学成 E-mail:sxc198301091126@163.com
  • 基金资助:
    CpG-ODN 经Akt/mTOR 抗UVB诱导的角质形成细胞凋亡的保护机制研究;InnVit 基因调控黑素细胞生物学功能及其分子机制

Quercetin′s protective effect against oxidative stress in and impact on biological activity of B10BR, an immortal mouse melanocyte cell line

  • Received:2009-04-22 Revised:2009-05-19 Online:2010-03-15 Published:2012-03-31

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摘要： 目的 探讨槲皮素保护鼠永生化黑素细胞（B10BR）对抗氧化应激的有效性及其对B10BR细胞生物学活性影响。方法 MTT法测定B10BR细胞存活率，流式细胞仪检测细胞凋亡率，倒置显微镜下观察细胞形态改变，并检测槲皮素对酪氨酸酶活性及黑素合成的影响。结果 经33.33 μmol/L槲皮素预处理24 h后细胞活性增高至（94.22 ± 3.36）%，此外黑素细胞的酪氨酸酶活性及黑素含量可分别增高至（107.15 ± 10.96）%和（111.85 ± 9.49）%，与过氧化氢处理组相比，差异均有统计学意义（P < 0.01）。流式细胞仪检测结果表明，槲皮素可抑制过氧化氢诱导的黑素细胞凋亡。结论 槲皮素抑制过氧化氢诱导黑素细胞凋亡的保护效应为其治疗白癜风提供一定的依据。

关键词: 槲皮素, 细胞凋亡, 黑素细胞, 过氧化氢酶

Abstract: Objective To investigate quercetin′s protective effect against oxidative stress in and impact on the biological activity of mouse B10BR melanocytes. Methods B10BR cells were cultured and treated with different concentrations of quercetin followed by additional culture. Then, cell viability was measured by using MTT assay, hydrogen peroxide-induced cell apoptosis by flow cytometry, and cell morphological changes by microscopy. The tyrosinase activity in and melanin synthesis by B10BR cells were measured by dopa oxidation assay and sodium hydroxide （NaOH）-lysis method, respectively. Results After treatment with quercetin of 33.33 μmol/L for 24 hours, the survival rate of B10BR cells reached （94.22 ± 3.36）%, tyrosinase activity（107.15 ± 10.96）%, and melanin content （111.85 ± 9.49）%. A significant difference was observed in tyrosinase activity and melanin content between hydrogen peroxide-induced and 33.33 μmol/L quercetin-treated B10BR cells and those only induced by hydrogen peroxide （both P < 0.01）. Flow cytometry revealed that quercetin inhibited hydrogen peroxide-induced apoptosis in melanocytes. Conclusion The protective effect of quercetin against hydrogen peroxide-induced apoptosis in melanocytes may provide a new idea for the treatment of vitiligo.

Key words: quercetin, Apoptosis, GSH-Px