中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (6): 397-399.

• 论著 • 上一篇    下一篇

MMP-2和MMP-9在多发性肌炎/皮肌炎患者单一核细胞中的表达

朱武 周艳宏 李静   

  1. 长沙中南大学湘雅医院皮肤科 长沙中南大学湘雅医院皮肤科
  • 收稿日期:2007-09-26 修回日期:2007-11-16 发布日期:2008-06-15
  • 通讯作者: 朱武 E-mail:zhuwu1970@yahoo.cn

Expression of matrix metalloproteinase-2 and -9 in the PBMC of patients with polymyositis and dermatomyositis

  

  • Received:2007-09-26 Revised:2007-11-16 Published:2008-06-15

PDF

107

摘要： 目的　探讨基质金属蛋白酶MMP-2和MMP-9 与多发性肌炎/皮肌炎（PM/DM）的关系。方法　采用实时荧光定量PCR和Western印迹方法分别检测PM/DM患者外周血单一核细胞（PMBC）中MMP-2和MMP-9的mRNA和蛋白表达水平。结果　MMP-2、MMP-9在PM/DM患者外周血PMBC中的mRNA表达水平明显高于正常人对照组，其中MMP-2为正常人对照组的2.41倍、MMP-9则为1.66倍。Western印迹检测发现MMP-2、MMP-9在PM / DM 患者外周血PMBC中的蛋白表达水平也明显高于正常人对照组，与其mRNA表达结果一致。结论　基质金属蛋白酶MMP-2和MMP-9可能在PM/DM的发生中起作用。

关键词: 多发性肌炎, 皮肌炎, 基质金属蛋白酶

Abstract: Objective To investigate the relation of the expressions of matrix metalloproteinase-2 （MMP-2） and -9 （MMP-9） with polymyositis/dermatomyositis （PM/DM）. Methods Blood samples were obtained from 8 patients with PM, 12 patients with DM and 10 normal human controls. Real-time quantitative RT-PCR and Western-blot were applied to examine the mRNA and protein expressions of MMP-2 and MMP-9 respectively in the peripheral blood mononuclear cells （PBMC） of these patients. Results The mRNA expression levels of MMP-2 and MMP-9 in the PBMC of patients with PM or DM were 2.41 and 1.66 folds as high as those of the normal controls, respectively; these differences were significant between the two groups . Increased expressions of MMP-2 and MMP-9 were also noted in the patients with PM or DM. Conclusion MMP-2 and MMP-9 may play a part in the development of polymyositis and dermatomyositis.

Key words: Polymyositis, Dermatomyositis, Matrix Metalloproteinases