中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (11): 739-741.

• 论著 • 上一篇    下一篇

CD70在SLE患者外周血T淋巴细胞中表达的研究

米向斌 邱贤文 谭国珍   

  1. 广州市南方医科大学珠江医院 广州市南方医科大学珠江医院 广州市中山大学第二医院皮肤科
  • 收稿日期:2007-09-27 修回日期:2007-10-23 发布日期:2008-11-15
  • 通讯作者: 米向斌 E-mail:mitaiyang@163.com

Expression of CD70 in T lymphocytes from patients with systemic lupus erythematosus

  

  • Received:2007-09-27 Revised:2007-10-23 Published:2008-11-15

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摘要： 目的 通过研究共刺激分子CD70在SLE患者外周血T淋巴细胞的表达水平，以及DNA甲基化抑制剂氮杂胞苷对正常人外周血T淋巴细胞表达CD70分子的影响，探讨DNA甲基化在SLE患者外周血T淋巴细胞表达CD70分子中的调控作用。方法 用流式细胞仪检测了10例活动期、10例非活动期SLE患者和10例正常人对照外周血T淋巴细胞以及甲基化抑制组（氮杂胞苷处理正常人T淋巴细胞）的CD70+CD4+ T淋巴细胞阳性率；用RT-PCR方法测定了各组外周血T淋巴细胞的CD70 mRNA转录水平。结果 正常人外周血CD4+CD70+ T淋巴细胞阳性率为14.55% ± 5.49%，活动期、非活动期SLE分别为85.25% ± 14.08%和77.65% ± 18.77%，甲基化抑制组为81.54% ± 8.71%，与正常人对照组相比，活动期、非活动期SLE患者和甲基化抑制组CD70+CD4+ T淋巴细胞阳性率均明显升高（P < 0.01），其外周血T淋巴细胞CD70 mRNA表达水平亦明显增加（P < 0.05）；SLE患者外周血CD70+CD4+ T淋巴细胞阳性率与SLE疾病活动度呈显著正相关（r = 0.72，P < 0.05）。结论 CD70的过度表达在SLE的免疫紊乱中起着重要的作用，并且CD70过度表达可以作为SLE疾病活动的一个指标。

关键词: 红斑狼疮, 系统性;T淋巴细胞;DNA甲基化;抗原, CD70

Abstract: Objective To detect the expression of CD70 in peripheral T lymphocytes of patients with systemic lupus erythematosus （SLE） and the effect of azacitidine, an inhibitor of DNA methylation, on it. Methods Blood samples were obtained from 10 patients with active SLE （SLEDAI score ≥5）, 10 patients with nonactive SLE （SLEDAI score < 5） and 10 normal human controls. Peripheral T lymphocytes were isolated and cultured for 72 hours. A part of the T lymphocytes from normal controls, which were cultured in the presence of azacitidine at 1 mol/L, served as the methylation-inhibited group. Semiquantitative reverse transcription PCR and flow cytometry were applied to detect the mRNA expression of CD70 and frequency of CD70+CD4+ cells in the cultured lymphocytes, respectively. Results The frequency of CD70+CD4+ lymphocytes was 14.55% ± 5.49% in normal control group, 85.25% ± 14.08% in active SLE group, 77.65% ± 18.77% in nonactive SLE group, and 81.54% ± 8.71% in methylation-inhibited group. Compared with the normal control group, a significant increase was observed in both the frequency of CD70+CD4+ lymphocytes （all P < 0.01） and the expression of CD70 expression （all P < 0.05） in other three groups. There was a positive correlation between the frequency of peripheral CD70+CD4+ lymphocytes and disease activity of SLE in patients （r = 0.72, P < 0.05）. Conclusions The elevated expression of CD70 appears to play a significant role in the immunologic disarrangement in SLE, and may act as a indicator of disease activity of this disease.

Key words: systemic lupus erythematosus, T-lymphocytes