中华皮肤科杂志 ›› 2008, Vol. 41 ›› Issue (10): 689-691.

• 论著 • 上一篇    下一篇

银屑病患者外周血白细胞mCD14、TLR4和核因子-κB的表达

徐丽敏 韩静倩 赵宏丽 等   

  1. 天津市长征医院 天津市长征医院 天津长征医院检验科
  • 收稿日期:2007-10-29 修回日期:2008-07-16 发布日期:2008-10-15
  • 通讯作者: 徐丽敏 E-mail:xulimin108@yahoo.com.cn

Expression of membrane CD14,Toll-like receptor 4 and nuclear factor-kappa B in peripheral blood leukocytes from patients with psoriasis vulgaris

  

  • Received:2007-10-29 Revised:2008-07-16 Published:2008-10-15

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摘要： 目的 探讨银屑病患者外周血白细胞mCD14、TLR4和NF-кB表达意义。方法 采用流式细胞仪检测银屑病患者32例和正常人29例外周血白细胞mCD14、TLR4和NF-кB的表达。结果 32例银屑病组外周血白细胞表面mCD14表达5.454 ± 2.78，对照组3.937 ± 1.964，差异有统计学意义（P < 0.05）。TLR4表达，银屑病组17.641 ± 18.120，对照组7.527 ± 8.574，差异有统计学意义（P < 0.01）。银屑病组外周血总白细胞活化NF-кB的表达为63.538 ± 8.650，对照组62.236 ± 6.72，差异无统计学意义（P > 0.05）。结论 银屑病患者中存在mCD14、TLR4表达异常，提示内毒素信号传导通路的异常激活也是感染诱发银屑病的病因之一。

关键词: CD14, TLR4, NF-кB, 银屑病, CD14；TLR4；NF-кB；银屑病

Abstract: Objective To investigate the expression of membrane CD14 （mCD14）, Toll-like receptor 4 （TLR4） and nuclear factor-kappa B （NF-кB） in peripheral blood leukocytes from patients with psoriasis vulgaris （PV）. Methods Blood samples were obtained from 29 normal controls and 32 patients with PV. Patient cohort consisted of 19 males and 13 females, with an average age of 30.28 ± 13.11 years, disease duration of 8.21 ± 10.21 years, and PASI score of 21.19 ± 5.85. Flow cytometry was used to measure the expression of mCD14, TLR4 and activated NF-кB in peripheral blood leukocytes in these subjects. Results The expressions of mCD14 and TLR4 in peripheral blood leukocytes were significantly higher in patients than in the normal controls （5.454 ± 2.78 vs 3.937 ± 1.964, P < 0.05; 17.641 ± 18.120 vs 7.527 ± 8.574, P < 0.01）. Although the expression of activated NF-кB in total blood leukocytes in patients was higher than that in normal controls, no statistical difference was noticed （63.538 ± 8.650 vs 62.236 ± 6.72, P > 0.05）. Conclusion There is an abnormal expression of mCD14 and TLR4 in patients with PV, suggesting that the abnormal activation of endotoxin signal transduction pathway may exert a role in the induction of psoriasis.

Key words: CD14;TLR4;NF-кB;psoriasis;CD14, TLR4, NF-кB, psoriasis