中华皮肤科杂志 ›› 2007, Vol. 40 ›› Issue (9): 546-548.

• 论著 • 上一篇    下一篇

NB-UVB和阿维A对角质形成细胞肝素结合表皮生长因子样生长因子mRNA的影响

罗素菊1, 彭振辉1, 郑焱1, 张路坤1, 曹振平1, 王国荣2   

  1. 1. 西安交通大学第二医院皮肤科, 710004;
    2. 陕西省人民医院普通外科
  • 收稿日期:2006-09-12 出版日期:2007-09-15 发布日期:2007-09-15
  • 通讯作者: 彭振辉, email:pengzhenhui2006@126.com E-mail:pengzhenhui2006@126.com
  • 基金资助:
    国家自然科学基金(30371295,30400387);陕西省自然科学基金(2004C_211)

Effects of acitretin and narrow-band UVB on the mRNA expression of heparin-binding epidermal growth factor like growth factor in normal human keratinocytes

LUO Su-ju1, PENG Zhen-hui1, ZHENG-Yan1, ZHANG Lu-kun1, CAO Zhen-ping1, WANG Guo-rong2   

  1. Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China
  • Received:2006-09-12 Online:2007-09-15 Published:2007-09-15

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摘要： 目的 探讨阿维A和窄谱中波紫外线(NB-UVB)对培养的正常人角质形成细胞增殖和肝素结合表皮生长因子样生长因子(HB-EGF)mRNA表达的影响.方法 用1μmol/L阿维A和/或100mJ/cm2NB-UVB处理正常人角质形成细胞12h后,用MTT法、RT-PCR和实时荧光定量RT-PCR法分别检测细胞增殖和HB-EGFmRNA表达的改变.结果 1μmol/L阿维A和100mJ/cm2NB-UVB单独作用时,均可抑制角质形成细胞的增殖和上调HB-EGFmRNA的表达,两者联合作用时,对角质形成细胞增殖的抑制和HB-EGFmRNA的诱导作用更强,分别为19.7%和8.6倍,之后依次为1μmol/L阿维A(14.4%,7.1倍)和100mJ/cm2NB-UVB(8.7%,2.5倍).结论 阿维A和/或NB-UVB处理后,可协同上调HB-EGF的表达,可能参与调节二者对角质形成细胞增殖的抑制作用.

关键词: 生长物质, 角蛋白细胞, 维生素A酸类, 紫外线

Abstract: Objective To investigate the effect of acitretin and narrow-band UVB(NB-UVB) on the cell proliferation and mRNA expression of heparin-binding epidermal growth factor like growth factor (HB-EGF) of cultured normal human keratinocytes.Methods Keratinocytes were isolated from the foreskin of young adults,and subjected to primary culture.After 3-5 passages,the keratinocytes were cultured with 1μmol/L acitretin for 12 h and/or irradiated with 100 mJ/cm2 NB-UVB.The proliferation potency of cells was detected by MTT colorimetric assay,and mRNA expression of HB-EGF by reverse transcription polymerase chain reaction(RT-PCR) and real-time quantitative RT-PCR.Results The treatment with acitretin and/or NB-UVB inhibited the keratinocyte growth and upregulated the mRNA expression of HB-EGF.The keratinocyte proliferation was inhibited by 14.4%,8.7% and 19.7%,and the mRNA expression of HB-EGF was elevated by 7.1,2.5 and 8.6 folds,respectively by 1μmol/L acitretin,100 mJ/cm2 NB-UVB and the combination of them.Conclusions Acitretin and NB-UVB could synergistically upregulate the expression of HB-EGF,which may be involved in the modulation of the inhibitory effect on the proliferation of keratinocytes.

Key words: Growth substances, Keratinocytes, Retinoids, Ultraviolet rays