中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (4): 190-192.

• 论著 • 上一篇    下一篇

白癜风相关基因-1的确认及其序列分析

李永伟, 张迪敏, 尉晓冬, 章一近, 许爱娥   

  1. 杭州市第三人民医院皮肤科 310009
  • 收稿日期:2005-08-08 发布日期:2006-04-15
  • 通讯作者: 许爱娥,email:xuaiehz@msn.com E-mail:xuaiehz@msn.com
  • 基金资助:
    浙江省自然科学基金资助项目(1303885)

Recognition of vitiligo associated protein VTT-1 gene and its sequence analysis

LI Yong-wei ZHANG Di-min, WEI Xiao-dong, ZHANG Yi-jin, XU Ai-e   

  1. Department of Dermatology, the Third People's Hospital of Hangzhou, Hangzhou 310009, China
  • Received:2005-08-08 Published:2006-04-15

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摘要： 目的 探讨白癜风患者黑素细胞VIT-1基因转录水平的表达情况,分析该基因序列的差异性。方法 用半定量RT-PCR法检测黑素细胞VIT-1基因表达水平,克隆VIT-1ORF cDNA全长并测序,序列差异由CLUSTALW(1.83)软件分析。结果 黑素细胞的VIT-1基因ORF含有81bp的内含子,为FBXO11的3'端的部分序列,白癜风患者黑素细胞VIT-1基因表达量下降。结论 VIT-1不是一个新基因,而是FBXO11,属于F-box蛋白家族成员。

关键词: 白癜风, 黑素细胞, F框蛋白质类, FBXO11, 白癜风相关基因-1

Abstract: Objective To investigate the expression of VIT-1 gene in melanocytes of patients with vitiligo, and to analyze the difference of its sequence. Methods The skin from the foreskins of healthy men by circumcision and from the non-lesional area on the buttocks of 5 patients were digested by dispase, then the epidermis and dermis were separated, and the melanocytes were isolated. Then we cultured the melanocytes from the controls in TICVA medium and those from the patients in TICVA medium supplemented with basic fibroblast growth factor (bFGF) and endothelin-1 (ET-1). The expression of VIT-1 gene was measured by RT-PCR, the full-length cDNA of VIT-1 ORF was cloned and sequenced, and sequence difference was analyzed by CLUSTAL W (1.83) software. Results The expression levels of VIT-1 gene were significantly lower in melanocytes from the patients than in those from the controls. An 81 bp-intron was found in the VIT-1 ORF. VIT-1 was a fragment of FBXO11, located at its 3' end. Conclusion VIT-1 gene is not a new gene, but a fragment of FBXO11, and a member of F-box protein family.

Key words: Vitiligo, Melanocytes, F-box proteins, VIT-1 gene, FBXO11