中华皮肤科杂志 ›› 2006, Vol. 39 ›› Issue (1): 29-31.

• 论著 • 上一篇    下一篇

慢性特发性荨麻疹患者单一核细胞IL-2、4、10受体mRNA的表达

高建明, 杨雪源, 解士海, 李遇梅, 姚煦, 陈敏, 陈志强   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2005-02-23 发布日期:2006-01-15

Expression of interleukin receptors IL-2R,IL-4R,IL-10R mRNA in patients with chronic idiopathic urticaria

GAO Jian-ming, YANG Xue-yuan, XIE Shi-hai, LI Yu-mei, YAO Xu, CHEN Min, CHEN Zhi-qiang   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2005-02-23 Published:2006-01-15

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摘要： 目的 探讨白介素2受体(IL-2R)、白介素4受体(IL-4R)、白介素10受体(IL-10R)在慢性特发性荨麻疹(CIU)患者外周血单一核细胞(PBMC)中的表达及与疾病的相关性.方法 收集30例确诊的CIU患者和30例正常人对照,收集PBMC,提取RNA.应用逆转录-聚合酶链反应(RT-PCR)方法检测研究对象IL-2R、IL-4R、IL-10RmRNA表达水平.结果 CIU患者IL-10RmRNA表达水平比健康人对照组增高,两组分别为0.806±0.072、0.373±0.027,差异有统计学意义(t=5.62,P<0.01).而IL-2R、IL-4RmRNA表达水平患者组与健康人对照组无统计学意义,分别为,IL-2R:0.688±0.357、0.670±0.527,t=1.16,P>0.05;IL-4R:0.679±0.055和0.549±0.049,t=1.77,P>0.05.结论 CIU患者IL-10RmRNA表达水平比健康人对照组增高,而IL-2R、IL-4RmRNA表达水平与健康人对照组无统计学意义.CIU患者存在T淋巴细胞细胞因子受体表达的异常.

关键词: 荨麻疹, 受体,白细胞介素2, 受体,白细胞介素4, 受体,白细胞介素10

Abstract: Objective To measure the mRNA expression of interleukin receptors (IL-2R?IL-4R and IL-10R) in peripheral blood mononuclear cells (PBMCs) from patients with chronic idiopathic urticaria (CIU).Methods Thirty CIU patients and 30 controls were enrolled in this study.PBMCs were separated from the peripheral blood specimens.Reverse transcription-polymerase chain reaction (RT-PCR) technique was applied to semi-quantitatively analyze the mRNA expression.Results The expression level of IL-10R mRNA was significantly increased in patients with CIU than that in the healthy controls,while that of IL-2R and IL-4R mRNA in PBMCs showed no significant difference.Conclusion Our results suggest that IL-10R might be involved in the pathogenesis of CIU.

Key words: Urticaria, Receptors,interleukin-2, Receptors,interleukin-4, Receptors,interleukin-10