中华皮肤科杂志 ›› 2005, Vol. 38 ›› Issue (6): 362-364.

• 论著 • 上一篇    下一篇

恶性黑素瘤FHIT基因蛋白的表达与肿瘤细胞增殖、凋亡关系的研究

李慎秋, 张春梅, 熊芬   

  1. 华中科技大学同济医学院附属同济医院皮肤科 武汉 430030
  • 收稿日期:2004-08-20 出版日期:2005-06-15 发布日期:2005-06-15

Expression of Fragile Histidine Triad Gene and its Association with Proliferation and Apoptosis of Tumor Cells in Cutaneous Malignant Melanoma

LI Shen-qiu, ZHANG Chun-mei, XIONG Fen   

  1. Department of Dermatology, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
  • Received:2004-08-20 Online:2005-06-15 Published:2005-06-15

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摘要： 目的 研究恶性黑素瘤(maligmant melanoma,MM)脆性组氨酸三联体(FHIT)基因的表达及与肿瘤细胞增殖、凋亡的关系。方法 用免疫组化SP法检测57例原发性MM和20例正常人皮肤中FHIT蛋白和增殖细胞核抗原的表达水平,用脱氧核糖核酸末端转移酶介导的缺口末端标记法(TUNEL)检测其细胞凋亡指数。结果 ①MM中,FHIT蛋白的表达水平明显低于正常人皮肤(P<0.001),增殖细胞核抗原的表达水平和细胞凋亡指数显著高于正常人皮肤(P<0.001);②FHIT蛋白、增殖细胞核抗原蛋白表达水平及细胞凋亡指数与MM患者的年龄、性别和肿瘤发生的部位无明显相关,而与其Clark分级和淋巴结转移状态明显相关(P<0.01);③MM与正常人皮肤相比,其细胞凋亡指数与增殖细胞核抗原的比值显著降低(P<0.001);④FHIT蛋白表达与MM细胞活跃增殖和凋亡受抑之间存在一定相关性(P<0.01)。结论 FHIT基因可能参与了MM肿瘤细胞的增殖、凋亡的调节,其表达下降可能与MM的发生、发展过程有关。

关键词: FHIT基因, 黑色素瘤, 增殖细胞核抗原, 细胞凋亡

Abstract: Objective To investigate the expression of fragile histid ine triad (FHIT)gene and its relationship with the proliferation and apoptosis of tumor cells in cutaneous malignant melanoma (CMM). Methods The expression of FHIT gene and PCNA were detected by streptavidin peroxidase method with skin specimens taken from 57 primary cutaneous melanoma and 20 normal controls.Apoptosis of tumor cells was detected by terminal deoxynuclneotidyl transferase mediated dUTP nick end labeling (TUNEL). Results The expression level of FHIT protei n was significantly lower in CMM than that in the normal skin tissue (P<0.001),on the contrary,the level of PCNA and apoptosis index were significantly higher in CMM than those in the normal skin tissue (P<0.001).The expression level of FHIT protein,PCNA and apoptosis index were not related to patients' age,sex and distribution of melanoma,but closely related to Clark level and lymphnode involvement of CMM.AI/PCNA ratio was significantly lower in CMM than that in the normal controls(P<0.01).The reduced expression of FHIT protein might berelated to over-proliferation and suppression of apoptosis of CMM cells (P<0.01). Conclusions FHIT gene may play a role in the regulation of proliferation and apoptosis of tumor cells in CMM.The reduced expression of FHIT protein may be related to the pathogenesis and development of CMM.

Key words: FHIT gene, Melanoma, Proliferating cell nuclear antigen, Apoptosis